跳至主要内容

Metabolite Profile of Lactic Acid-Fermented Soymilk

Traditional soy foods produced in Asia are known to have lipid metabolism-modulating effects. Soy protein and isoflavones are the major bioactive components in soy foods. Clinical analyses and meta-analyses have demonstrated the lipid metabolism-modulating effects of combined isoflavones and soy protein.

Soymilk is the simplest soy food, but some individuals find the flavor unpalatable. Thus, we used soymilk fermented by lactic acid bacteria of vegetable origin to make its flavor more palatable. Lactic acid fermentation converts the isoflavone glycosides in soymilk to isoflavone aglycones, enhancing their absorption in the intestine. Previous studies have shown that isoflavone aglycone-rich soymilk fermented by Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4408L (F4408) has stronger lipid metabolism-modulating effects than isoflavone aglycone-poor soymilk fermented by Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4404L (F4404). As such, fermentation products other than isoflavone aglycones may also exert lipid metabolism-modulating effects. Therefore, the present study aimed to compare the metabolites in soymilk (SM), F4404, and F4408 via a metabolomics approach.

In the study, two fermented soymilk products were prepared from soymilk via lactic acid fermentation for 15 h using Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4404L (F4404) or TUA-4408L (F4408). The soymilk metabolome analyses were carried out at Human Metabolome Technologies Inc. (HMT, Tsuruoka, Japan). Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) was carried out using an Agilent CE Capillary Electrophoresis System. Liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS) was carried out using an Agilent 1200 series RRLC System SL equipped with an Agilent LC/MSD TOF (Agilent Technologies). Mass spectrometry (MS) analysis was carried out in both positive and negative ion electrospray ionization (ESI) modes of detection.

The results indicated that various aglycones including isoflavones, flavones, flavonols, and flavanones increased in both F4404 and F4408 compared with SM. The increases in isoflavone and flavanone aglycones were greater in F4408 than in F4404. Some types of dipeptides and free amino acids, especially ornithine, increased in both fermented soymilks. The increase in free amino acids, especially ornithine, was higher in F4408 than in F4404. Thus, F4408 exhibited stronger glycosidase and protease activities than F4404. Functional components produced by lactic acid fermentation are known to improve lipid metabolism.

In conclusion, it is suggested that not only isoflavone aglycones but also other functional components exert lipid metabolism-modulating effects in fermented soymilks, especially soymilk fermented using TUA-4408L.


Article by Maki Kobayashi, et al, from Japan.

Full access: http://mrw.so/4qCr3o

评论

发表评论

此博客中的热门博文

Electron Spin and Proton Spin in the Hydrogen and Hydrogen-Like Atomic Systems

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52202#.VIj7tMnQrzE Author(s) Stanisław Olszewski * Affiliation(s) Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland . ABSTRACT The mechanical angular momentum and magnetic moment of the electron and proton spin have been calculated semiclassically with the aid of the uncertainty principle for energy and time. The spin effects of both kinds of the elementary particles can be expressed in terms of similar formulae. The quantization of the spin motion has been done on the basis of the old quantum theory. It gives a quantum number n = 1/2 as the index of the spin state acceptable for both the electron and proton ...
发表评论
阅读全文

Incorporation of High-Altitude Balloon Experiment in High School Science Classrooms

High-altitude balloon is a balloon, filled usually with helium or hydrogen that ascends into an area called “near space” or stratosphere. The most common type of high-altitude balloons are weather balloons. Other purposes include use as a platform for experiments in the upper atmosphere. Modern balloons generally contain electronic equipment such as radio transmitters, cameras, or satellite navigation systems, such as GPS receivers. The mission of the High-Altitude Balloon Experiment (HABE) is to acquire supporting data, validate enabling technologies, and resolve critical acquisition, tracking, and pointing (ATP) and fire control issues in support of future space-based precision pointing experiments. The use of high-altitude balloons offers a relatively low-cost, low-vibration test platform, a recoverable and reusable payload, worldwide launch capability, and a 'near- space' emulation of the future space systems operational scenarios. More recently, several university...
1 条评论
阅读全文

An Empirical Study on Microbial Load and Acidity in Raw Milk Produced in Malayer and Nahavand Cities, Iran 2012

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=49618#.VBk-qVfHRK0 An Empirical Study on Microbial Load and Acidity in Raw Milk Produced in Malayer and Nahavand Cities, Iran 2012 Author(s) Mohammad Rezaei , Ali Asghar Behzadi , Ali Akbar Malekirad , Ahmad Fattahi , Mohamad Hadi Farahzadi , Mohammad Sarmadi , Hasan Mohammadi Aghamirlou , Reza Ali Fallahzadeh Affiliation(s) Department of Food Safety and Hygiene, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran . Department of Food Safety and Hygiene, School of Health, Yazd University of Medical Sciences, Yazd, Iran . Department of Biology, Payame Noor University, Tehran, Iran . Department of Occupational Health Engineering, School of Health, Shahid Sadoughi University of Medical Sci-ences, Yazd, Iran . Department...
发表评论
阅读全文