Electrorotation: A Spectroscopic Imaging Approach to Study the Alterations of the Cytoplasmic Membrane
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52953#.VK3k_8nQrzE Author(s) Adalberto Bonincontro 1 , Gianfranco Risuleo 2* Affiliation(s) 1 Dipartimento di Fisica, Sapienza Università di Roma, Rome, Italy . 2 Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy . ABSTRACT Electrorotation (ER) is a technique allowing the characterization of the surface properties of a variety of supra-molecular aggregates and living cultured cells as well as cellular organelles and biological materials in general. In particular, this technique allows measuring two important physical parameters of the cell membrane: specific capacitance and specific conductance. These parameters are strictly related to the structure/function relationships of the biological membrane; ER becomes thus a powerful means to investigate a number of phenomena involving the membrane integrity. These phenomena may o...