DNA Barcodes in Fig Cultivars (Ficus carica L.) Using ITS Regions of Ribosomal DNA, the psbA-trnH Spacer and the matK Coding Sequence
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53128#.VLXUC8nQrzE Author(s) Carlos Castro 1 , Alejandro Hernandez 2 , Luis Alvarado 2 , Dora Flores 2 Affiliation(s) 1 Agribiotecnología de Costa Rica S.A., Alajuela, Costa Rica . 2 Instituto Tecnológico de Costa Rica (ITCR), Cartago, Costa Rica . ABSTRACT Molecular markers provide a useful method for genotype characterization and allow a high precision determination of the genetic relationship between cultivars and varieties. A system based on DNA sequences—which is known as DNA barcoding—will choose one or several standard loci which can be sequenced and compared to differentiate between species. In this research, the ITS, matK, and trnH-psbA sequences were evaluated for the molecular identification of seven F. carica genotypes, generating complete sequences for the first two loci, but unable to produce bidirectional sequences by using the...