Expression of the Genes OsNRT1.1, OsNRT2.1, OsNRT2.2, and Kinetics of Nitrate Uptake in Genetically Contrasting Rice Varieties
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53865#.VNm0dSzQrzE Author(s) Osmário J. L. Araújo 1 , Milena S. Pinto 1 , Marcus V. L. Sperandio 1 , Leandro A. Santos 1 , Elvia M. L. M. Stark 1 , Manlio S. Fernandes 1 , André Marques dos Santos 2* , Sonia Regina de Souza 2 Affiliation(s) 1 Plant Nutrition Laboratory, Department of Soil, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil . 2 Plant Biochemistry Laboratory, Department of Chemistry, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil . ABSTRACT Four genetically contrasting rice varieties (IAC-47, Bico Ganga, Arroz de Revenda and Manteiga) according to Random Amplified Polymorphic DNA (RAPD) analysis were assessed regarding expression of the genes OsNRT1.1, OsNRT2.1 and OsNRT2.2 and the nitrate uptake kinetics parameters (Km and Vmax). Up to 250-fold increases in the induction of gene expression after ni...