Liquid Chromatography-Tandem Mass Spectrometry Methods for Determination of Delamanid in Mouse Plasma and Lung
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53301#.VLydj8nQrzE Author(s) Yukihiro Hirao * , Toshihisa Koga , Noriyuki Koyama , Yoshihiko Shimokawa , Ken Umehara Affiliation(s) Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima-shi, Japan . ABSTRACT Sensitive and selective liquid chromatography-tandem mass spectrometry methods have been developed and validated for the determination of delamanid in mouse plasma and lung. Sample preparation involved liquid-liquid extraction with diethyl ether for plasma, or protein precipitation followed by liquid-liquid extraction with tert -butyl methyl ether for lung homogenate. Chromatographic separation was performed on a reversed phase column with a linear gradient elution using purified water-formic acid (1000:2, v/v) and methanol-formic acid (1000:2, v/v) at a flow rate of 0.2 mL/min. Detection was performed on a triple-quadrupole ...