Reversible Immobilization of Chelating Affinity Surfactants on Reversed Phase Adsorbents for Protein and Peptide Separations under Metal Affinity Chromatography
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50437#.VD9CHFfHRK0 Author(s) Omar González-Ortega 1,2 , Roberto Guzmán 1 Affiliation(s) 1 Chemical and Environmental Engineering Department, The University of Arizona, Tucson, USA . 2 Bioprocess Engineering Department, Autonomous University of San Luis Potosí, San Luis Potosí, México . ABSTRACT Alkyl-bound silica was modified using chelating surfactants and the resulting adsorbent was used in immobilized metal affinity chromatography of proteins and peptides. Brij-76, a non-ionic amphiphilic surfactant with an alkyl moiety and an ethylene oxide chain, was reversible adsorbed to alkyl silica (C 18 ). The hydroxyl group at the end of the ethylene oxide chain was ch...