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博文

目前显示的是标签为“Polymerase Chain Reaction (PCR)”的博文

Genetic Variations and Cultivar Purity within the G. hirsutum Cultivars via RAPD Marker

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54086#.VO1zGyzQrzE Author(s)   Farah Raheel 1 , Muhammad Kauser Nawaz Shah 2 , Muhammad Shahid Iqbal 2,3 , Ijaz Rasool Noorka 4 , Zareen Sarfraz 5   Affiliation(s) 1 Government Postgraduate College (w), Multan, Pakistan . 2 PMAS Arid Agricultural University, Rawalpindi, Pakistan . 3 Ayub Agriculture Research Institute, Faisalabad, Pakistan . 4 Department of Plant Breeding and Genetics, University College of Agriculture, University of Sargodha, Sargodha, Pakistan . 5 National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan . ABSTRACT To assess genetic variation within a cultivar, four commercial varieties with five individual G. hirsutum genotypes and their bulk were assayed with selected forty 10-mer RAPDs per cultivar via PCR. CIM-473 cultivar showed amplification of 321 bands in total per primer with an average of 8 bands. A...

Relevance of MICA Alleles Matching Rate and Posttransplant Rejection in Clinical LRD Organ Transplantations

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53309#.VLyjNcnQrzE Author(s)    Liang He 1 , Gang Ji 2 , Zhang Zhang 2 , Chunsheng Xu 2 , Weizhong Wang 2 , Boquan Jin 2 , Guanglong Dong 3*   Affiliation(s) 1 Department of General Surgery, Lanzhou Military District Hospital in Urumqi, Urumqi, China . 2 Department of Gastrointestinal Surgery, State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China . 3 Department of General Surgery, The General Hospital of the People’s Liberation Army, Beijing, China . ABSTRACT Objective: Recipients usually undergo posttransplant rejection in HLA-identical transplantations. Recent studies have shown that MHC class I related chain A (MICA) has been found to be associated with allograft survival. The goal of this study is to investigate the correlation between matching rate of MICA alleles an...