跳至主要内容

Genetic Variations and Cultivar Purity within the G. hirsutum Cultivars via RAPD Marker

Read  full  paper  at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54086#.VO1zGyzQrzE

ABSTRACT
To assess genetic variation within a cultivar, four commercial varieties with five individual G. hirsutum genotypes and their bulk were assayed with selected forty 10-mer RAPDs per cultivar via PCR. CIM-473 cultivar showed amplification of 321 bands in total per primer with an average of 8 bands. A range of similarity i.e.; 80% - 100% within the cultivar has been observed for minimum and maximum respectively. CIM-448 showed amplification of 300 bands in total per primer with an average of 7.5 bands. Maximum similarity of 95% and minimum similarity of 84% was observed within the cultivar. In CIM-1100 the total numbers of fragments amplified are 304, with the average of 7.6 bands per primer and are 12% - 20% polymorphic. Similarly, in NIAB-78 the total numbers of fragments amplified were 310, with the average of 7.7 bands per primer. The reliability of RAPD technique for finding genetic resemblances is indicated by the mentioned results. Numerous groups within 5 individual genotypes bulk among 4 cultivars of G. hirsutum L. have been through cluster analysis which was by the unweighted pair group method of arithmetic means (UPGMA). Dendrograms have been used for showing cultivar relationship.
 
Cite this paper
Raheel, F. , Shah, M. , Iqbal, M. , Noorka, I. and Sarfraz, Z. (2015) Genetic Variations and Cultivar Purity within the G. hirsutum Cultivars via RAPD Marker. American Journal of Plant Sciences, 6, 392-404. doi: 10.4236/ajps.2015.62045.
 
References
[1]Iqbal, M.J., Aziz, N., Saeed, N.A., Zafar, Y. and Malik, K.A. (1997) Genetic Diversity of Some Elite Cotton Varieties by RAPD Analysis. Theoretical and Applied Genetics, 94, 139-144.
 
[2]Tatineni, V., Cantrell, R.G. and Davis, D.D. (1996) Genetic Diversity in Elite Cotton Germplasm Determined by Morphological Characteristics and RAPDs. Crop Sciecne, 36, 186-192.
 
[3]Linos, A.A., Bebeli, P.J. and Kaltsikes, P.J. (2002) Cultivar Identification in Upland Cotton Using RAPD Markers. Australian Journal of Agriculture Research, 53, 637-642.
http://dx.doi.org/10.1071/AR01129
 
[4]Raheel, F., Saima, S., Dasti, A.A. and Mahmood, Z. (2005) Morpho-Taxonomic Study of Some Old World Cotton Species. Pakistan Journal of Plant Science, 11, 45-50.
 
[5]Sudhanshu, J. (1997) Studies on Combining Ability in Intra and Interspecific Crosses of Cotton. Crop Research Hisar, 14, 91-95.
 
[6]Lerner, I.M. (1954) Genetic Homeostasis. John Wiley and Sons, New York.
 
[7]Gustafsson, A. (1948) The Effect of Heterozygosity on Variability and Vigour. Hereditos, 32, 263-286. http://dx.doi.org/10.1111/j.1601-5223.1946.tb02779.x
 
[8]Zhu, Y., Chen, H., Fan, J., Wang, Y., Li, Y., Chen, J., Fan, J., Yang, S., Hu, L., Leung, H., Mew, T.W., Teng, P.S., Wang, Z. and Mundt, C.C. (2000) Genetic Diversity and Disease Control in Rice. Nature, 406, 718-722.
http://dx.doi.org/10.1038/35021046
 
[9]Rommens, J.M., Iannuzzi, M.C., Kerem, B., Drumm, M.L., Melmer, G., Dean, M., Rozmahel, R., Cole, J., Kennedy, D., Hidaka, N., Zsiga, M., Buchwald, M., Riordan, J.R., Tsui, L.C. and Collins, F.S. (1989) Identification of the Cystic Fibrosis Gene: Chromosome Walkihg and Jumping. Science, 245, 1059-1065.
http://dx.doi.org/10.1126/science.2772657
 
[10]Wallace, M.R., Marchuk, D.A., Anderson, R., Letcher, R., Odeh, H.M., Saulino, A.M., Fountain, J.W., Bereton, A., Nicholson, J., Mitchell, A.L., Brownstein, B.H. and Collins, F.S. (1990) Type 1 Neurofibromatosis Gene: Identification of a Large Transcript Disrupted in Three NF1 Patients. Science, 249, 181-186.
 
[11]Paterson, A.H., De Verna, J.W., Lanini, B. and Tanksley, S.D. (1990) Fine Mapping of Quantitative Trait Loci Using Selected Overlapping Recombinant Chromosomes, in an Interspecies Cross of Tomato. Genetics, 124, 735-742.
 
[12]Bowman, D.T., May, O.L. and Calhoun, D.S. (1996) Genetic Base of Upland Cotton Cultivars Released between 1970 and 1990. Crop Science, 36, 577-581.
http://dx.doi.org/10.2135/cropsci1996.0011183X003600030008x
 
[13]Iqbal, M.J. and Rayburn, A.L. (1994) Stability of RAPD Markers for Determining Cultivar-Specific DNA Profiles in Rye (Seeale cereal L.). Euphytica, 75, 215-220. http://dx.doi.org/10.1007/BF00025606
 
[14]Doyle, J.J. and Doyle, J.H. (1990) Isolation of Plant DNA from Fresh Tissue. Focus, 12, 13-15.
 
[15]Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor.
 
[16]Williams, C.E. and St. Clair, D.A. (1993) Phenetic Relationships and Levels of Variability Detected by Restriction Fragment Length Polymorphism and Random Amplified DNA Analysis of Cultivated and Wild Accessions of Lycopersicon esculentum. Genome, 36, 619-630.
http://dx.doi.org/10.1139/g93-083
 
[17]Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor.
 
[18]Sokal, R.R. and Sneath, P.H.A. (1963) Principles of Numerical Taxonomy. W.H. Freeman, San Francisco.
 
[19]Rani, V., Parida, A. and Raina, S.N. (1995) Random Amplified Polymorphic DNA (RAPD) Markers for Genetic Analysis in Micropropagated Plants of Populus deltoides Marsh. Plant Cell Reports, 14, 459-462.
http://dx.doi.org/10.1007/BF00234055
 
[20]Soniya, E.V., Banerjee, N.S. and Das, M.R. (2001) Genetic Analysis of Somaclonal Variation among Callus-Derived Plants of Tomato. Current Science, 80, 1213-1215.
 
[21]Thormann, C.E., Ferreira, M.E., Camargo, L.E.A., Tivang, J.G. and Osborn, T.C. (1994) Comparison of RFLP and RAPD Markers to Estimating Genetic Relationship within and among Cruciferous Species. Theoretical and Applied Genetics, 88, 973-980.
http://dx.doi.org/10.1007/BF00220804
 
[22]Fouilloux, G. and Bannerot, H. (1988) Selection Methods in Common Bean (Phaseolus vulgaris L.). In: Gepts, P., Ed., Genetic Resources of Phaseolus Beans, Klüwer Academic Publishers, Dordrecht, 503-541.
http://dx.doi.org/10.1007/978-94-009-2786-5_21
 
[23]Franca, L.T., Carrilho, E. and Kist, T.B. (2002) A Review of DNA Sequencing Techniques. Quarterly Reviews of Biophysics, 35, 169-200. http://dx.doi.org/10.1017/S0033583502003797
 
[24]Multani, D.S. and Lyon, B.R. (1995) Genetic Fingerprinting of Australian Cotton Cultivars with RAPD Markers. Genome, 38, 1005-1008. http://dx.doi.org/10.1139/g95-132
 
[25]Nie, Y.C., Zuo, K.J., Zhang, X.L., Feng, C.D. and Liu, J.L. (2000) Application of RAPD Markers in Differentiating Germplasm Lines from 4x Gossypium arboreum x G. anomalum x G. hirsutum. Scientia Agricultura Sinica, 33, 25- 29.
 
[26]Vas-Esbroeck, G.A. and Bowman, D.T. (1998) Cotton Germplasm Diversity and Its Importance to Cultivar Development. Journal of Cotton Sciences, 2, 121-129.
 
[27]Tokatlidis, I.S., Tsikrikoni, C., Tsialtas, J.T., Lithourgidis, A.S. and Bebeli, P.J. (2008) Variability within Cotton Cultivars for Yield, Fiber Quality and Physiological Traits. The Journal of Agricultural Science, 146, 483-490. http://dx.doi.org/10.1017/S0021859608007867
 
[28]Jain, A., Bhatta, S., Banga, S.S., Prakash, S. and Lakshmikumaran, M. (1994) Potential Use of Random Amplified Polymorphic DNA (RAPD) Technique to 201 Study the Genetic Diversity in Indian Mustard (Brassica juncea) and Its Relationship to Heterosis. Theoretical and Applied Genetics, 88, 116-122.
http://dx.doi.org/10.1007/BF00222403
 
[29]Tafvizi, F., Sheidai, M., Farahanei, F., Nourmohammadi, Z. and Alishah, O. (2010) Somaclonal Variation in Three Tetraploid Cotton (Gossypium hirsutum L.) Cultivars. Cotton Research Center, Gorgan.   eww150225lx
标签：

评论

发表评论

此博客中的热门博文

A Comparison of Methods Used to Determine the Oleic/Linoleic Acid Ratio in Cultivated Peanut (Arachis hypogaea L.)

Cultivated peanut ( Arachis hypogaea L.) is an important oil and food crop. It is also a cheap source of protein, a good source of essential vitamins and minerals, and a component of many food products. The fatty acid composition of peanuts has become increasingly important with the realization that oleic acid content significantly affects the development of rancidity. And oil content of peanuts significantly affects flavor and shelf-life. Early generation screening of breeding lines for high oleic acid content greatly increases the efficiency of developing new peanut varieties. The objective of this study was to compare the accuracy of methods used to classify individual peanut seed as high oleic or not high oleic. Three hundred and seventy-four (374) seeds, spanning twenty-three (23) genotypes varying in oil composition (i.e. high oleic (H) or normal/not high oleic (NH) inclusive of all four peanut market-types (runner, Spanish, Valencia and Virginia), were individually tested ...
发表评论
阅读全文

Location Optimization of a Coal Power Plant to Balance Costs against Plant’s Emission Exposure

Fuel and its delivery cost comprise the biggest expense in coal power plant operations. Delivery of electricity from generation to consumers requires investment in power lines and transmission grids. Placing a coal power plant or multiple power plants near dense population centers can lower transmission costs. If a coalmine is nearby, transportation costs can also be reduced. However, emissions from coal plants play a key role in worsening health crises in many countries. And coal upon combustion produces CO 2 , SO 2 , NO x , CO, Metallic and Particle Matter (PM10 & PM2.5). The presence of these chemical compounds in the atmosphere in close vicinity to humans, livestock, and agriculture carries detrimental health consequences. The goal of the research was to develop a methodology to minimize the public’s exposure to harmful emissions from coal power plants while maintaining minimal operational costs related to electric distribution losses and coal logistics. The objective was...
发表评论
阅读全文

Evaluation of the Safety and Efficacy of Continuous Use of a Home-Use High-Frequency Facial Treatment Appliance

At present, many home-use beauty devices are available in the market. In particular, many products developed for facial treatment use light, e.g., a flash lamp or a light-emitting diode (LED). In this study, the safety of 4 weeks’ continuous use of NEWA TM , a high-frequency facial treatment appliance, every alternate day at home was verified, and its efficacy was evaluated in Japanese individuals with healthy skin aged 30 years or older who complained of sagging of the facial skin.  Transepidermal water loss (TEWL), melanin levels, erythema levels, sebum secretion levels, skin color changes and wrinkle improvement in the facial skin were measured before the appliance began to be used (study baseline), at 2 and 4 weeks after it had begun to be used, and at 2 weeks after completion of the 4-week treatment period (6 weeks from the study baseline). In addition, data obtained by subjective evaluation by the subjects themselves on a visual analog scale (VAS) were also analyzed. Fur...
发表评论
阅读全文