Development and Validation of a Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in a Formulation by RP-HPLC
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=48944#.VJjZRcCAM4 Author(s) P. B. N. Prasad , K. Satyanarayana , G. Krishnamohan Affiliation(s) Central Drugs Testing Laboratory, Hyderabad, India . NATCO Pharma R&D, Hyderabad, India . Department of Pharmacy, JNTUH, Hyderabad, India . ABSTRACT Present study was aimed to develop and validate a reverse-phase high-performance liquid chromatography method for simultaneous determination of sitagliptin phosphate and metformin hy-drochloride in a marketed formulation. The drug separation was performed on Hibar-240, Li-chrosphere-100 C18 ODS (250 × 4.6 mm, 5 μm) column, at a flow rate of 1 mL/min. The mobile phase used was a mixtur...