Construction of EST-SSR Databases for Effective Cultivar Identification and Their Applicability to Complement for Lettuce (Lactuca sativa L.) Distinctness Test
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53239#.VLh24MnQrzE Author(s) Jee-Hwa Hong 1,2 , Yong-Sham Kwon 3 , Raghvendra Kumar Mishra 2 , Doo Hwan Kim 2* Affiliation(s) 1 Seed Testing & Research Center, Korea Seed & Variety Service, Ministry of Agriculture, Food and Rural Affairs, Gimcheon, Korea . 2 Department of Bioresources and Food Science, Konkuk University, Seoul, Korea . 3 Department of Genetic Engineering, College of Natural Resources and Life Science, Dong-A University, Busan, Korea . ABSTRACT The objectives of this study were to construct a database of expressed sequence tag (EST)-simple sequence repeat (SSR) markers to identify lettuce cultivars. A set of 370 EST-SSR primer pairs were applied for fingerprinting the lettuce cultivars. Fifty-eight EST-SSR markers showed hy-per-variability and were able to differentiate 92 cultivars. A total of 176 polymorphic amplified fragm...