Forward-Light-Scattering Characterization of Pre-Crystalline Aggregates in Crystallizing Lysozyme Solutions
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47148#.VGBLt2fHRK0 Author(s) Takashi Wakamatsu Affiliation(s) Department of Electrical and Electronic System Engineering, Ibaraki National College of Technology, Ibaraki, Japan . ABSTRACT We present a method to characterize lysozyme pre-crystalline aggregates using a forward-light-scattering technique, which is highly sensitive to protein aggregates. The static light scattering properties at small angles of crystallizing lysozyme aggregates are discussed, and related to the crystallization conditions based on the concentration of added precipitant NaCl. Lysozyme solutions that started to crystallize because of precipitant exhibit...