Differentiation of Avian Pathogenic Escherichia coli Strains from Broiler Chickens by Multiplex Polymerase Chain Reaction (PCR) and Random Amplified Polymorphic (RAPD) DNA
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50640#.VEccgVfHRK0 Author(s) Dirgam Ahmad Roussan 1* , Hana Zakaria 2 , Ghassan Khawaldeh 1 , Ibrahim Shaheen 1 Affiliation(s) 1 Provimi ( a Cargill Company ), Amman, Jordan . 2 The University of Jordan, Animal Production Department, Amman, Jordan . ABSTRACT We examined 50 Escherichia coli ( E. coli ) strains isolated from broiler chickens between January 2013 to March 2014 in order to evaluate the epidemiological prevalence of avian pathogenic E. coli (APEC) in Jordan by multiplex PCR and random amplification of polymorphic DNA (RAPD) tests. The multiplex polymerase chain reaction (PCR) which was used as tentative criteria of APEC...