Determination of Sibutramine and Its N-Desmethyl Metabolites in Human Plasma Using HPLC Coupled with Tandem Mass Spectroscopy
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47149#.VGBLuWfHRK0 Author(s) Mohammad Al-Bayari Affiliation(s) Chemistry Department, Faculty of Science, University of Tabuk, Tabuk, KSA . ABSTRACT A sensitive and selective liquid chromatographic method coupled with tandem mass spectrometry has been developed and validated for the determination of Sibutramine and its N -desmethyl metabolites in human plasma. The analytes Sibutramine, N-monodesmethylsibutramine, N-N-didesmethylsibutramine and Bisoprolol (internal standard) have been separated on a reversed phase column (Merck, Purospher RP-C18, 30 × 4.0 (mm), 3 μm) using a mobile phase which consists of an aqueous solution 20 mM...