Performance Characteristics of ELISA to Detect Bovine Viral Diarrhea Virus (BVDV) Antibodies Using Colostrum
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54067#.VOBb7izQrzE Author(s) Caitlin J. Jenvey 1 , Andrew M. Weir 2 , Michael P. Reichel 1 , Peter D. Cockcroft 1 Affiliation(s) 1 School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, Australia . 2 Eltham District Veterinary Service, Eltham, New Zealand . ABSTRACT Colostrum contains substantially higher concentrations of immunoglobulins (Igs) when compared with serum or milk, which may improve the diagnostic sensitivity of an antibody ELISA when using colostrum. In this study, BVD was used as a model to identify the performance characteristics of colostrum and to assess the potential for increased ELISA sensitivity when compared with serum. Blood and colostrum samples were collected from cows within two dairy cattle herds: a previously infected and BVD-vaccinated Holstein-Friesian (positive herd) herd...