Scientific Research Publishing

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54086#.VO1zGyzQrzE Author(s)   Farah Raheel 1 , Muhammad Kauser Nawaz Shah 2 , Muhammad Shahid Iqbal 2,3 , Ijaz Rasool Noorka 4 , Zareen Sarfraz 5   Affiliation(s) 1 Government Postgraduate College (w), Multan, Pakistan . 2 PMAS Arid Agricultural University, Rawalpindi, Pakistan . 3 Ayub Agriculture Research Institute, Faisalabad, Pakistan . 4 Department of Plant Breeding and Genetics, University College of Agriculture, University of Sargodha, Sargodha, Pakistan . 5 National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan . ABSTRACT To assess genetic variation within a cultivar, four commercial varieties with five individual G. hirsutum genotypes and their bulk were assayed with selected forty 10-mer RAPDs per cultivar via PCR. CIM-473 cultivar showed amplification of 321 bands in total per primer with an average of 8 bands. A...

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53865#.VNm0dSzQrzE Author(s)    Osmário J. L. Araújo 1 , Milena S. Pinto 1 , Marcus V. L. Sperandio 1 , Leandro A. Santos 1 , Elvia M. L. M. Stark 1 , Manlio S. Fernandes 1 , André Marques dos Santos 2* , Sonia Regina de Souza 2   Affiliation(s) 1 Plant Nutrition Laboratory, Department of Soil, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil . 2 Plant Biochemistry Laboratory, Department of Chemistry, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil . ABSTRACT Four genetically contrasting rice varieties (IAC-47, Bico Ganga, Arroz de Revenda and Manteiga) according to Random Amplified Polymorphic DNA (RAPD) analysis were assessed regarding expression of the genes OsNRT1.1, OsNRT2.1 and OsNRT2.2 and the nitrate uptake kinetics parameters (Km and Vmax). Up to 250-fold increases in the induction of gene expression after ni...

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52422#.VJdtZcCAM4 Author(s)    Habib Ahmad 1 , Ayaz Khan 1* , Khushi Muhammad 1* , Muhammad Shahid Nadeem 1 , Waqar Ahmad 1 , Sajjad Iqbal 2 , Aniqa Nosheen 1 , Nazia Akbar 1 , Israr Ahmad 1 , Youxiong Que 3 Affiliation(s) 1 Department of Genetics, Hazara University, Manshera, Pakistan . 2 Institute of Chemical and Biological Sciences, Department of Botany, University of the Gujrat, Gujrat, Pakistan . 3 Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China . ABSTRACT This paper reports the findings regarding morphology, DNA markers and f...

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51087#.VFMhNWfHRK0 Author(s) Neal Dwaine Teaster , Robert Edward Hoagland * Affiliation(s) US Department of Agriculture-Agricultural Research Service, Crop Production Systems Research Unit, Stoneville, USA . ABSTRACT qPCR (quantitative polymerase chain reaction) and random amplified polymorphic DNA (RAPD) were utilized to investigate genetic stability of Palmer amaranth cloned plants over 10 generations. DNA from original parent Palmer amaranth plants (grown from seeds) was re-analyzed using qPCR, and confidence levels for determining Δ Δ Ct (threshold crossing) values were established. ANOVA was used to determine variation (margi...