Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52255#.VI5MdcnQrzE Author(s) Jian Liu , Feifei Ge , Houbing Ju , Dequan Yang , Xin Li , Jian Wang , Peihong Liu , Jinping Zhou * Affiliation(s) Shanghai Animal Disease Control Center, Shanghai, China . ABSTRACT A dual real-time quantitative polymerase chain reaction assay (drtqPCR) was established to detect and differentiate between Porcine circovirus-2a (PCV-2a) and Porcine circovirus-2b (PCV-2b). Genotype-specific primer sets and probes were designed by using sequence data published for different PCV-2 strains. Specificity and sensitivity of the drtqPCR were examined by using PCV-2 isolates with known genotype. Among 367 ...
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