Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50726#.VEmy8lfHRK0 Author(s) Xiping Yuan , Hong Li , Chongrui Wang , Bo Hong Affiliation(s) Fishery Environmental Monitoring Station, Fisheries Research Institute of Hunan Province, Changsha, China . ABSTRACT Approximately 200 mg of fin tissue from each specimen was cut into pieces and treated with a gradient of ethanol (65%, 70%, 75%, 80%, 85%, 90%, 95% and 100%) and then DNA was extracted from formalin fish fin tissues. Agarose gel visualization of the DNA confirmed DNA extracted. MtDNA cyt b gene banding pattern of samples with agarose gel was clearly visible and could be used to be sequenced. In terms of DNA purity, the 260...
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