Volume Change of the Random Coil to Folded Conformational Transition of Thermomyces lanuginosus Xylanase at 24°C and pH = 7.0 via Application of the Clausius-Clapeyron Equation
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51532#.VG2uCGfHRK0 Author(s) Heather N. H. Wilks , Tara M. Arrington , Billy Mark Britt Affiliation(s) Department of Chemistry and Biochemistry, Texas Woman’s University, Denton, Texas, USA . ABSTRACT A partial phase diagram characterizing the conformational change that occurs in Thermomyces lanuginosus xylanase as it is slowly heated in 150 mM sodium phosphate (pH = 7.0) has been con-structed from slow-scan-rate differential scanning calorimetry measurements. The Clausius-Clapeyron equation was applied to determine an associated volume change of -205 L·mol -1 at 24°C, the equilibrium transition temperature at 1.0 atm pre...