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Volume Change of the Random Coil to Folded Conformational Transition of Thermomyces lanuginosus Xylanase at 24°C and pH = 7.0 via Application of the Clausius-Clapeyron Equation

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A partial phase diagram characterizing the conformational change that occurs in Thermomyces lanuginosus xylanase as it is slowly heated in 150 mM sodium phosphate (pH = 7.0) has been con-structed from slow-scan-rate differential scanning calorimetry measurements. The Clausius-Clapeyron equation was applied to determine an associated volume change of -205 L·mol-1 at 24°C, the equilibrium transition temperature at 1.0 atm pressure. This value is in excellent agreement with that predicted using a previously published [1] empirical equation for calculating the hydro-dynamic radius if the transition is regarded as from a random coil to a functional, folded state and with the assumption that the hydrodynamic radius is a good approximation of the true random coil radius. The existence of a low-temperature random coil is confirmed by circular dichroism and dynamic light scattering measurements. Thus, at 24°C and 1.0 atm pressure the enzyme appears to fold from a random coil to a functional, folded form as it is slowly heated.
Cite this paper
Wilks, H. , Arrington, T. and Britt, B. (2014) Volume Change of the Random Coil to Folded Conformational Transition of Thermomyces lanuginosus Xylanase at 24°C and pH = 7.0 via Application of the Clausius-Clapeyron Equation. Journal of Biophysical Chemistry, 5, 134-142. doi: 10.4236/jbpc.2014.54015. 
 

[1] Wilkins, D.K., Grimshaw, S.B., Receveur, V., Dobson, C.M., Jones, J.A. and Smith, L.J. (1999) Hydrodynamic Radii of Native and Denatured Proteins Measured by Pulse Field Gradient NMR Techniques. Biochemistry, 38, 16424-16431.
http://dx.doi.org/10.1021/bi991765q
[2] Bodnar, M.A. and Britt, B.M. (2006) Transition State Characterization of the Low- to Physiological-Temperature Nondenaturational Conformational Change in Bovine Adenosine Deaminase by Slow-Scan-Rate Differential Scanning Calorimetry. Journal of Biochemistry and Molecular Biology, 39, 167-170.
http://dx.doi.org/10.5483/BMBRep.2006.39.2.167
[3] Younvanich, S.S. and Britt, B.M. (2006) The Stability Curve of Hen Egg White Lysozyme. Protein and Peptide Letters, 13, 769-762.
http://dx.doi.org/10.2174/092986606777841163
[4] Hollowell, H.N., Younvanich, S.S., McNevin, S.L. and Britt, B.M. (2007) Thermodynamic Analysis of the Low- to Physiological-Temperature Nondenaturational Conformational Change of Bovine Carbonic Anhydrase. Journal of Biochemistry and Molecular Biology, 40, 205-211.
http://dx.doi.org/10.5483/BMBRep.2007.40.2.205
[5] McNevin, S.L., Nguyen, D.T. and Britt, B.M. (2008) Partial Phase Diagram of Aqueous Bovine Carbonic Anhydrase: Analyses of the Pressure-Dependent Temperatures of the Low- to Physiological-Temperature Nondenaturational Conformational Change and of Unfolding to the Molten Globule State. Journal of Biomolecular Structure & Dynamics, 26, 263-272.
http://dx.doi.org/10.1080/07391102.2008.10507242
[6] Ijeoma, O., Hollowell, H.N., Bodnar, M.A. and Britt, B.M. (2008) Thermodynamic Analysis of the Nondenaturational Conformational Change of Baker’s Yeast Phosphoglycerate Kinase at 24°C. Archives of Biochemistry and Biophysics, 478, 206-211.
http://dx.doi.org/10.1016/j.abb.2008.07.005
[7] Singh, S., Madlala, A.M. and Prior, B.A. (2006) Thermomyces lanuginosus: Properties of Strains and Their Hemicellulases. FEMS Microbiology Reviews, 1, 3-16.
[8] Azad, K., Hossain, F. and Halim, M.A. (2013) Screening of Cellulase, Pectinase and Xylanase Activities and Optimization of Radial Mycelial Growth of Two Thermophilic Fungi. Bangladesh Journal of Botany, 42, 207-213.
[9] Gruber, K., Klintschar, G., Hayn, M., Schlacher, A., Steine, W. and Kratky, C. (1998) ThermophilicXylanase from Thermomyces lanuginosus: High-Resolution X-Ray Structure and Modeling Studies. Biochemistry, 37, 13475-13485.
http://dx.doi.org/10.1021/bi980864l
[10] Meersman, F., Smeller, L. and Heremans, K. (2006) Protein Stability and Dynamics in the Pressure-Temperature Plane. Biochimica et Biophysica Acta (BBA)—Proteins and Proteomics, 1764, 346-354.
http://dx.doi.org/10.1016/j.bbapap.2005.11.019
[11] Winter, R. and Dzwolak, W. (2004) Temperature-Pressure Configurational Landscape of Lipid Bilayers and Proteins. Cellular and Molecular Biology, 50, 397-417                                               eww141120lx
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