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博文

目前显示的是标签为“Tissue Culture”的博文

Cell-Specific Effects of Ethanol on Nitric Oxide Synthase Induced by Inflammatory Mediators

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52879#.VKtMh8nQrzE Author(s)     Patience O. Obih 1* , Barry J. Potter 2 Affiliation(s) 1 College of Pharmacy, Xavier University of Louisiana, New Orleans, LA, USA . 2 Department of Physiology, Louisiana State University Health Sciences Center, New Orleans, LA, USA . ABSTRACT To examine the pro- and anti-oxidant properties of nitric oxide (NO) production in alcoholic liver disease, we compared the effects of ethanol pretreatment (24 hrs, 100 mM concentration in a dedicated CO 2 incubator) on the induction of inducible NO synthase and its activity in a cell culture system. We employed two types of liver cells as mode...

Advances in D. melanoxylon Investigations towards Tissue Culture: Problems and Limitations

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51850#.VHfPlGfHRK0 Author(s) Washa B. Washa 1 , A. M. S. Nyomora 2 Affiliation(s) 1 Mkwawa University College of Education (MUCE), Iringa, Tanzania . 2 Botany Department, University of Dar es salaam, Dar es salaam, Tanzania . ABSTRACT The first attempt on D. melanoxylon tissue culture was conducted from 2010 to 2013 at a high level of expectations. A total of 500 seeds were sterilized at different concentration of reagents and inoculated at different strength of the Murashige and Skoog medium for germination to obtain disease free explants for callus induction trials. A total of 400 nodal segments obtained from germinated seeds were ...