EWS Knockdown and Taxifolin Treatment Induced Differentiation and Removed DNA Methylation from p53 Promoter to Promote Expression of Puma and Noxa for Apoptosis in Ewing’s Sarcoma
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50940#.VFCDDFfHRK0 Author(s) Mohammad Motarab Hossain , Swapan Kumar Ray * Affiliation(s) Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA . ABSTRACT Ewing’s sarcoma is a pediatric tumor that mainly occurs in soft tissues and bones. Malignant characteristics of Ewing’s sarcoma are correlated with expression of EWS oncogene. We achieved knockdown of EWS expression using a plasmid vector encoding EWS short hairpin RNA (shRNA) to increase anti-tumor mechanisms of taxifolin (TFL), a new flavonoid, in human Ewing’s sarcoma cells in culture and animal models. Immunoflu...