Molecular Cloning and Characterization of a Candidate Plant Growth-Related and Time-Keeping Constitutive Cell Surface Hydroquinone (NADH) Oxidase (ENOX1) from Arabidopsis lyrata
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53731#.VNB6JyzQrzE Author(s) Xiaoyu Tang * , Laura M. C. Ades , Dorothy M. Morré , D. James Morré * Affiliation(s) MorNuCo, Inc., Purdue Research Park, West Lafayette, IN, USA . ABSTRACT ENOX (ECTO-NOX) proteins are proteins of the external surface of the plasma membrane that catalyze oxidation of both NADH and hydroquinones as well as carry out protein disulfidethiol interchange. They exhibit both prion-like and time-keeping (clock) properties. The oxidative and interchange activities alternate to generate a regular period of 24 min in length. Here we report the cloning, expression, and characterization of a plant candidate constitutive ENOX (CNOX or ENOX1) protein from Arabidopsis lyrata . The gene encoding the 335 (165) amino acid protein is found in accession XP-002882467. Functional motifs characteristics of ENOX proteins previously identified by s...