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博文

目前显示的是标签为“AFLP”的博文

A Breakdown of Obligate Mutualism on a Small Island: An Interspecific Hybridization between Closely Related Fig Species (Ficus pumila and Ficus thunbergii) in Western Japan

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53241#.VLh5HsnQrzE Author(s)     Lum Tsai 1 , Hiroshi Hayakawa 2 , Tatsuya Fukuda 3 , Jun Yokoyama 4*   Affiliation(s) 1 Graduate Schools of Science and Engineering, Yamagata University, Yamagata, Japan . 2 National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan . 3 Faculty of Agriculture, Kochi University, Nankoku, Kochi, Japan . 4 Faculty of Science, Yamagata University, Yamagata, Japan . ABSTRACT Ficus (Moraceae) is a well-known group with specific pollination mutualisms, and hybridization is considered to be rare. Here, we report the presence of interspecific hybrids between Ficus pumila L. and F. thunbergii Maxim. on Okinoshima, a small island offshore of Shikoku, western Japan. AFLP (amplified fragment length polymorphism of genomic DNA) data suggested that more than one-fourth of individuals of morphological F. pumila...

AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50291#.VDSRelfHRK0 Author(s)   Te-Hua Hsu 1 , Yue Ning 2 , Jin-Chywan Gwo 1* Affiliation(s) 1 Department of Aquaculture, National Taiwan Ocean University, Taiwan . 2 Fisheries Research Institute of Fujian, Xiamen, China . ABSTRACT Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals of a species. Recently, in spite of the development of high-throughput genotyping technologies, SNPs have been applied as markers for population genetic and high-density genetic mapping. However, the high costs of SNPs discovery and genotyping assay limit the applications of SNP markers in non-model organisms. In this study, we present ...