Effect of the Inorganic Nitrogen Source in the Expression of Nitrite Reductase (NirA) in Thermosynechococcus elongatus BP-1
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51361#.VGVeRWfHRK0 Author(s) Maite Buxens , María J. Llama , Juan L. Serra * Affiliation(s) Enzyme and Cell Technology Group, Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Bilbao, Spain . ABSTRACT Nitrite reductase (NirA, EC 1.7.7.1) from the thermophilic, unicellular, non-N2-fixing cyanobacte-rium Thermosynechococcus elongatus BP-1 has been cloned and expressed in Escherichia coli . Analysis by SDS-PAGE of the pure recombinant protein (His6NirA) showed two protein bands, one of 58 kDa (corresponding to the theoretical His6NirA molecular mass...