Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51850#.VHfPlGfHRK0 Author(s) Washa B. Washa 1 , A. M. S. Nyomora 2 Affiliation(s) 1 Mkwawa University College of Education (MUCE), Iringa, Tanzania . 2 Botany Department, University of Dar es salaam, Dar es salaam, Tanzania . ABSTRACT The first attempt on D. melanoxylon tissue culture was conducted from 2010 to 2013 at a high level of expectations. A total of 500 seeds were sterilized at different concentration of reagents and inoculated at different strength of the Murashige and Skoog medium for germination to obtain disease free explants for callus induction trials. A total of 400 nodal segments obtained from germinated seeds were ...
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