跳至主要内容

Hippocampal High-Frequency Stimulation Inhibites the Progression of Rapid Kindling-Induced Seizure in Rats

Epilepsy is a central nervous system disorder (neurological disorder) in which nerve cell activity in the brain becomes disrupted, causing seizures or periods of unusual behavior, sensations and sometimes loss of consciousness. Seizure symptoms can vary widely. It can be treated by drugs or surgeries. However, pharmaco-resistant epilepsy patients are poorly controlled or their seizures are refractory to drug treatment, then resective surgery is frequently a promising therapy in this population; while not all the patients meet the eligibility criteria for the surgical treatment.

In recent years, deep brain stimulation has been investigated in clinical studies and animal studies as an alternative treatment, but the optimal stimulation parameters remain an issue. The present study was designed to investigate the effect of unilateral high-frequency stimulation (HFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats, and compared the results with those of low-frequency stimulation previously published by the authors’ group.

In this study, the authors used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK; n = 7) received only RK stimulus, while the treated group (HFS-hRK; n = 9) received also HFS (biphasic square wave pulses, 130 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing 78% (p < 0.01) of the animals receiving HFS treatment were still not fully kindled staying in stages 0 - III (p < 0.01). HFS group needed a higher number of stimulations to achieve stage III (p <0.05) with respect to control group. However, no significant differences in the cumulative daily afterdischarge duration were observed. HFS did not present significant differences compared with LFS in any of studied parameters.

In conclusion, the findings suggest that unilateral HFS applied on hippocampus effectively inhibited the epileptogenic process induced by hippocampal rapid kindling. According to the comparative results about hippocampal rapid kindled animals stimulated with HFS and LFS (5 Hz), there wasn’t conclusive information on which treatment was most efficient.

Article by Belen Gori, et al, from Argentina.

Full access: http://mrw.so/1mVMLH
Image by ilovepiano, from Flickr-cc.


评论

发表评论

此博客中的热门博文

Electron Spin and Proton Spin in the Hydrogen and Hydrogen-Like Atomic Systems

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52202#.VIj7tMnQrzE Author(s) Stanisław Olszewski * Affiliation(s) Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland . ABSTRACT The mechanical angular momentum and magnetic moment of the electron and proton spin have been calculated semiclassically with the aid of the uncertainty principle for energy and time. The spin effects of both kinds of the elementary particles can be expressed in terms of similar formulae. The quantization of the spin motion has been done on the basis of the old quantum theory. It gives a quantum number n = 1/2 as the index of the spin state acceptable for both the electron and proton ...
发表评论
阅读全文

Effects of Karate Training on Basic Motor Abilities of Primary School Children

“You never attack first in karate” might be the best conclusion of karate, which is a martial art practiced typically without weapons. It’s reported that karate has a long history for several hundred years, but the modern karate was spread to the whole Japan from Okinawa in the early part of 20th century. Now it has become one of the most widely practiced martial art forms in the world. Usually, it’s divided into Kihon, Kata and Kumite. As for the beginners, Kihon is more suitable for them because it involves basic techniques. Due to karate consists of dynamic offensive and defensive techniques using all parts of the body to their maximum advantage, the best understanding of true karate practice is the perfection of oneself through the perfection of the art. It not only develops coordination, quickens reflexes, and builds stamina, but also develops composure, a clearer thought process, deeper insight into one’s mental capabilities, and more self-confidence. So many researchers stu...
发表评论
阅读全文

Fermented Brown Sugar Residue Prolongs the Caenorhabditis elegans Lifespan via DAF-16

Fermented brown sugar residue (FBSR) is generated in the course of purifying biomass ethanol from the yeast fermentation products of brown sugar. It contains abundant nutrition and is used in various industrial applications including as fertilizer and feed. Meanwhile, brown sugar has attracted attention as a health food and is thought to prevent cardiovascular diseases, hypertension, and brain stem diseases. Furthermore, polyphenol, contained in brown sugar, reduces oxidative stress and inhibits glucose absorption. DAF-16 is a transcription factor regulated downstream of the insulin signaling pathway and is one of the main factors contributing to aging and lifespan in C. elegans . Therefore, to determine the precise roles of the DAF-16 transcription factor in stress tolerance and life span, it is necessary to elucidate the signaling pathways involved in DAF-16 activation. This study was designed to elucidate the molecular mechanisms controlling the physiological stress toleran...
发表评论
阅读全文