跳至主要内容

Cryopreservation of Seeds and Embryos of Jatropha curcas L.

Read  full  paper  at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53262#.VLiDsMnQrzE

ABSTRACT
Jatropha curcas is a species with a variety of uses. It is grown primarily for oil for biodiesel, but also has agronomic and medicinal applications. Two methods were evaluated for cryopreservation of seeds and zygotic embryos of J. curcas: desiccation followed by rapid immersion of seeds and embryos in liquid nitrogen (LN, -196°C), and vitrification of zygotic embryos. Prior to cryo-preservation, seeds were manually scarified and the moisture content (MC) of seeds and embryos was determined. Explants were disinfected after cryopreservation. Seed germination after LN exposure was 100%. Plantlet development was better in sand substrate than that in vitro. Survival of zygotic embryos after cryopreservation was also 100%, without significant differences between treatments. Optimal development (100%) and plantlet length (51.77 mm) were observed with embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservation. Zygotic embryos subjected to the vitrification procedure did not withstand LN exposure. Survival data for non-cryopreserved embryos after each step of the vitrification procedure provided information about embryo tolerance to cryoprotectants.
 
Cite this paper
Prada, J. , Aguilar, M. , Abdelnour-Esquivel, A. and Engelmann, F. (2015) Cryopreservation of Seeds and Embryos of Jatropha curcas L.. American Journal of Plant Sciences, 6, 172-180. doi: 10.4236/ajps.2015.61020.
 
References
[1]Machado, R. and Suárez, J. (2009) Performance of Three Provenances of Jatropha curcas in the Germoplasm Bank of the EEPF “Indio Hatuey”. Pastos y Forrajes, 32, 29-37.
 
[2]CATIE (Centro Agronómico Tropical de Investigación y Enseñanza) (2003) Arboles de Centroamérica: Un manual para extensionistas. Cordero, J. y Boshier, D. Turrialba, CR, 1079 p.
 
[3]Jiménez, R. and Martínez, G. (1994) Redescripción de Jatrophaandrieuxii Muell. Arg. (Euphorbiaceae), una especie endémica del sur de México. Acta Botánica Mexicana, 26, 27-32.
 
[4]Martínez, G., Jiménez, R., Cruz, R., Juárez, A., Garcia, R., Cervantes, A. and Mejía, R. (2002) Los géneros de la familia Euphorbiaceae en México. Anales del Instituto de Biología, UNAM. Ser Bot: UNAM Serie Botanica, 73, 155-281.
 
[5]Kone-Bamba, D., Pelissier, Y., Ozouko, Z. and Ouao, D. (1987) Etude de l’activité hémostatique de quinze plantes médicinales de la “Pharmacopée Traditionnelle Ivoirienne”. Plantes Médicinales et Phytothérapie, 21, 121-130.
 
[6]Heller, J. (1996) Physic Nut. Jatropha curcas L. Promoting the Conservation and Use of Underutilized and Neglected Crops. 1. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, 66 p.
 
[7]Grainge, M. and Ahmed, S. (1988) Handboook of Plants with Pest-Control Properties. John Wiley & Sons, Hoboken, 470 p.
 
[8]Mukherjee, P., Varhney, A., Johnson, T.S. and Jha, T.B. (2011) Jatropha curcas: A Review on Biotechnological Status and Challenges. Plant Biotechnology Reports, 5, 197-215.
 
[9]Deng, Z.J., Xiang, Z.Y., Cheng, H.Y., Li, Y.J. and Song, S.Q. (2008) Preliminary Study on Development, Germination and Desiccation Tolerance of Jatropha curcas (Euphorbiaceae). Acta Botanica Yunnanica, 30, 355-359.
 
[10]Joker, D. and Jepsen, J. (2003) Jatropha curcas L. Seed Leaflet, No 83 August Danida Forest Seed Centre. Demark, 81-108.
 
[11]GonzalezArnao, M.T. and Engelmann, F. (2013) Introducción a la conservación ex situ de los recursos genéticos vege- tales. In: GonzalezArnao, M.T. and Engelmann, F., Eds., Crioconservación de plantas en América Latina y el Caribe, Editorial Instituto Interamericano de Cooperación para la Agricultura, San José, 39-48.
 
[12]Engelmann, F. (1991) In Vitro Conservation of Tropical Plant Germplasm—A Review. Euphytica, 57, 227-243. http://dx.doi.org/10.1007/BF00039669
 
[13]Reed, B.M. (2008) Plant Cryopreservation: A Practical Guide. Springer, New York.
http://dx.doi.org/10.1007/978-0-387-72276-4
 
[14]Hine-Gómez, A. and Abdelnour-Esquivel, A. (2013) Establecimiento in vitro de arándano (Vaccinium corymbosum L). Tecnología en Marcha, 26, 64-71.
 
[15]Engelmann, F. (2011) Use of Biotechnologies for the Conservation of Plant Biodiversity. In Vitro Cellular & Developmental Biology—Plant, 47, 5-16. http://dx.doi.org/10.1007/s11627-010-9327-2
 
[16]International Seed Testing Association (ISTA) (2008) International Ruler For Seed Testing: The Germination Test. Seed Science and Technology, p. 7.
 
[17]Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
 
[18]Matsumoto, T., Sakai, A. and Yamada, K. (1994) Cryopreservation of in Vitro Grown Apical Meristems of Wasabi (Wasabia japonica) by Vitrification and Subsequent High Plant Regeneration. Plant Cell Reports, 13, 442-446. http://dx.doi.org/10.1007/BF00231963
 
[19]Sakai, A., Kobayashi, S. and Oiyama, I. (1990) Cryopreservation of Nucellar Cells of Navel Orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by Vitrification. Plant Cell Reports, 9, 30-33.
 
[20]Di Rienzo, J.A., et al. (2008) Infostat: Manual del usuario versión 2008. Coordinado por Di Rienzo, J.A.-1ed.-Brujas, Cordoba, ISBN 98-987-591-100-9.
 
[21]Silva, R., Camillo, J. and Pereira, S. (2012) A Method for Seedling Recovery in Jatropha curcas after Cryogenic Exposure of the Seeds. Revista de Biologia Tropical, 60, 473-482.
 
[22]Toral, O., Iglesias, J., Oca, S., Sotolongo, J., Garcia, S. and Torsti, M. (2008) Jatrpha curcas L., a Tree Species with Energetic Potential in Cuba. Pastos y Forrajes, 31, 191-207.
 
[23]Verma, K. and Gaur, A. (2009) Jatropha curcas L.: Substitute for Conservation Energy. World Journal of Agricultural Sciences, 5, 552-556.
 
[24]Abdelnour-Esquivel, A., Villalobos, V. and Engelmann, F. (1992) Cryopreservation of Zygotic Embryos of Coffea spp. Cryoletters, 13, 197-302.
 
[25]Normah, N., Choo, K., Yap, V. and Zeti, M. (2011) In Vitro Conservation of Malaysian Biodiversity-Archievements, Challenges and Future Directions. In Vitro Cellular & Developmental Biology—Plant, 47, 26-36. http://dx.doi.org/10.1007/s11627-010-9306-7
 
[26]Abdelnour-Esquivel, A. and Aguilar, M.E. (2013) Crioconservación de germoplasma vegetal en Costa Rica. In: Gon- zalezArnao, M.T. and Engelmann, F., Eds., Crioconservación de plantas en America Latina y el Caribe, Editorial Instituto Interamericano de Cooperación para la Agricultura, San José, 113-126.
 
[27]Scocchi, A., Dieringer, E., Mroginski, E. and Mroginski, L. (2004) Conservación de semillas de cedro australiano (Toona ciliata). Plant Genetic Resources Newsletter, 137, 1-4.
 
[28]Scocchi, A.M. and Rey, H.Y. (2004) Conservación de germoplasma in vitro. In: Echenique, V., Rubinstein, C. and Mroginski, L., Eds., Biotecnología y mejoramiento vegetal, Ediciones INTA, Buenos Aires, 179-185.
 
[29]Abdelnour-Esquivel, A., Rojas, G. and Alfaro, U. (2007) Estudios preliminares para la crioconservación de especies forestales arbóreas. Tecnología en Marcha, 20, 98-103.
 
[30]Aguilar, M.E. and Abdelnour-Esquivel, A. (2010) Desarrollo de modelos para la crioconservación de semillas y material clonal de especies forestales de Costa Rica en peligro de extinción y seleccionadas en programas de mejoramiento genético. Boletin de Ciencia y Tecnología No. 99, San José, CONICIT. http://163.178.205.6/boletin/boletin99/PalabrasMariaElenaAguilar.html
 
[31]Meryman, H.T., Williams, R.J. and Douglas, S.J. (1977) Freezing Injury from Solution Effects and Its Prevention by Natural or Artificial Cryoprotection. Cryobiology, 14, 287-302.
http://dx.doi.org/10.1016/0011-2240(77)90177-8
 
[32]Goldfarb, M., Duarte, M. and Mata, M. (2010) Armazenamento criogênico de sementes de pinhão manso (Jatropha curcas L.) Euphorbiaceae. Revista Biotemas, 23, 27-33.                                         eww150116lx
标签：

评论

发表评论

此博客中的热门博文

Does Immigration Promote the Investment of the Monopolistic Firm?

In the present paper, we examine the effect of increasing uncertainty of immigrants’ growth on the optimal timing of investment of a firm that has a monopolistic power over the labor market. It is revealed that when the uncertainty of immigrants’ growth is more than a threshold level, increasing uncertainty of immigrants’ growth accelerates the optimal timing of firms’ investment and enhances the economic growth, even if the uncertainty of immigrants’ growth is formulated by the geometric Brownian motion, which is in sharp contrast to the standard result that an increase in the uncertainty postpones the optimal timing. With an increase in the immigrants over the past ten years, workforces in the host countries have been growing significantly to the extent that the immigrants represent 70% of the increase in the workforce in Europe, and 47% in the United States as OECD indicates. In the present paper, we attempted to investigate the effect of increased uncertainty caused by the growi...
发表评论
阅读全文

Education Policy Implementation: A Mechanism for Enhancing Primary Education Development in Zanzibar

Education is one of the fundamental rights of individuals; therefore, the government of a country needs to develop and strengthen educational policy and quality as well as to ensure that everyone has equal access to basic education. The improvement of access and quality of education in the world is becoming as an essential factor in development, whereas the basic education (primary school), is acknowledged as a foundation of the higher educational development for every country. To fulfill this goal, governments introduce several policies and procedures; however, it requires some reforms and participation from the politician, policymakers, and other stakeholders to re-examine educational policy so that it can lead to multiplication and betterment of the reforms. Educational reforms actually focus on accountability. A positive educational development and reform is very challenging and needs more effort and strategy on how to use and utilize the resources effectively as such it can achie...
发表评论
阅读全文