跳至主要内容

Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.)

Read  full  paper  at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53312#.VLyjPMnQrzE

ABSTRACT
Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-tolerance identification in cotton.
 
Cite this paper
Cui, Y. , Lu, X. , Wang, D. , Wang, J. , Yin, Z. , Fan, W. , Wang, S. and Ye, W. (2015) Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.). Agricultural Sciences, 6, 78-86. doi: 10.4236/as.2015.61007.
 
References
[1]Parida, A.K. and Das, A.B. (2005) Salt Tolerance and Salinity Effects on Plants: A Review. Ecotoxicology and Environment Safety, 60, 324-349.http://www.ncbi.nlm.nih.gov/pubmed/15590011
 
[2]Ward, J.M., Hirschi, K.D. and Sze, H. (2003) Plants Pass the Salt. Trends Plant Science, 8, 200-201.
http://www.ncbi.nlm.nih.gov/pubmed/12758034
 
[3]Zhu, J.K. (2002) Salt and Drought Stress Signal Transduction in Plants. Annual Review Plant Biology, 53, 247-273. http://www.ncbi.nlm.nih.gov/pubmed/12221975
http://dx.doi.org/10.1146/annurev.arplant.53.091401.143329
 
[4]Ghosh, D., Tripathi, Y.K., Mujamdar, M.K., Kumar, R.V. and Prakash, V. (2006) Evaluation of Salt Tolerance Capability of Cotton (Gossypium hirsutum L.) Germplasm. Tropical Agriculture, 83, 30-36.
 
[5]Meloni, D.A., Oliva, M.A., Martinez, C.A. and Cambraia, J. (2003) Photosynthesis and Activity of Superoxide Dismutase, Peroxidase and Glutathione Reductase in Cotton under Salt Stress. Environmental and Experimental Botany, 49, 69-76.
http://dx.doi.org/10.1016/S0098-8472(02)00058-8
 
[6]Li, X.X., Liu, B.X., Guo, Z.T., Chang, Y.X., He, L., et al. (2013) Effects of NaCl Stress on Photosynthesis Characteristics and Fast Chlorophyll Fluorescence Induction Dynamics of Pistaciachinensis Leaves. Ying Yong Sheng Tai Xue Bao, 24, 2479-2484. http://www.ncbi.nlm.nih.gov/pubmed/24417104
 
[7]Ashraf, M. (2002) Salt Tolerance of Cotton: Some New Advances. Critical Reviews in Plant Sciences, 21, 1-30. http://www.ingentaconnect.com/content/tandf/bpts/2002/00000021/00000001/art00001
http://dx.doi.org/10.1016/S0735-2689(02)80036-3
 
[8]Tripathi, A.K. and Tripathi, S. (1999) Changes in Same Physiological and Biochemical Characters in Albizialebbek as Bio-Indicators of Heavy Metal Toxicity. Journal of Environmental Biology, 20, 93-98.
 
[9]Sun, C.H., Du, W., Cheng, X.L., Xu, X.N., Zhang, Y.H., et al. (2010) The Effects of Drought Stress on the Activity of Acid Phosphatase and Its Protective Enzymes in Pigweed Leaves. African Journal of Biotechnology, 9, 825-833.
 
[10]Furtado, R.F., Mano, A.R.D., Alves, C.R., de Freitas, S.M. and Filho, S.M. (2007) Effect of Salinity on the Germination of Cotton Seeds. Revista Ciencia Agronomica, 38, 224-227.
 
[11]Meneses, C.H.S.G., Bruno, R.D.A., Fernandes, P.D., Pereira, W.E., de Morais Lima, L.H.G., de Andrade Lima, M.M. and Vidal, M.S. (2011) Germination of Cotton Cultivar Seeds under Water Stress Induced by Polyethyleneglycol-6000. Scientia Agricola, 68, 131-138.
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200001&lng=en&nrm=
iso&tlng=en http://dx.doi.org/10.1590/S0103-90162011000200001
 
[12]Smith, L.H., Keys, A.J. and Michael, C.W.E. (1995) Striga hermonthica Decreases Photosynthesis in Zea mays through Effects on Leaf Cell Structure. Journal of Experimental Botany, 46, 759-765.
http://dx.doi.org/10.1093/jxb/46.7.759
 
[13]Yu, L.N. and Pei, J. (2011) Application of Differential Proteomics in Mechanism Research of Acupuncture. Zhong Xi Yi Jie He Xue Bao, 9, 819-823.
http://www.ncbi.nlm.nih.gov/pubmed/21849141
 
[14]Zhang, G.W., Lu, H.L., Zhang, L., Chen, B.L. and Zhou, Z.G. (2011) Salt Tolerance Evaluation of Cotton (Gossypium hirsutum) at Its Germinating and Seedling Stages and Selection of Related Indices. Ying Yong Sheng Tai Xue Bao, 22, 2045-2053.
http://www.ncbi.nlm.nih.gov/pubmed/22097366
 
[15]Mechin, V., Damerval, C. and Zivy, M. (2007) Total Protein Extraction with TCA-Acetone. Methods in Molecular Biology, 355, 1-8. http://www.ncbi.nlm.nih.gov/pubmed/17093296
 
[16]Wu, X., Gong, F. and Wang, W. (2014) Protein Extraction from Plant Tissues for 2DE and Its Application in Proteomic Analysis. Proteomics, 14, 645-658.
http://www.ncbi.nlm.nih.gov/pubmed/24395710
http://dx.doi.org/10.1002/pmic.201300239
 
[17]Kottapalli, K.R., Payton, P., Rakwal, R., Agrawal, G.K., Shibato, J., Burow, M. and Puppala, N. (2008) Proteomics Analysis of Mature Seed of Four Peanut Cultivars Using Two-Dimensional Gel Electrophoresis Reveals Distinct Differential Expression of Storage, Anti-Nutritional, and Allergenic Proteins. Plant Science, 175, 321-329. http://dx.doi.org/10.1016/j.plantsci.2008.05.005
 
[18]Law, R.D., Crafts-Brandner, S.J. and Salvucci, M.E. (2001) Heat Stress Induces the Synthesis of a New Form of Ribulose-1, 5-bisphosphate Carboxylase/Oxygenase Activase in Cotton Leaves. Planta, 214, 117-125. http://www.ncbi.nlm.nih.gov/pubmed/11762161
http://dx.doi.org/10.1007/s004250100592
 
[19]Spreitzer, R.J. (2003) Role of the Small Subunit in Ribulose-1,5-bisphosphate Carboxylase/Oxygenase. Archives of Biochemistry and Biophysics, 414, 141-149.
http://www.ncbi.nlm.nih.gov/pubmed/12781765
http://dx.doi.org/10.1016/S0003-9861(03)00171-1
 
[20]Chaves, M.M., Flexas, J. and Pinheiro, C. (2009) Photosynthesis under Drought and Salt Stress: Regulation Mechanisms from Whole Plant to Cell. Annals of Botany, 103, 551-560. http://www.ncbi.nlm.nih.gov/pubmed/18662937
http://dx.doi.org/10.1093/aob/mcn125
 
[21]Van der Straeten, D., Rodrigues-Pousada, R.A., Goodman, H.M. and Van Montagu, M. (1991) Plant Enolase: Gene Structure, Expression, and Evolution. Plant Cell, 3, 719-735.
http://www.ncbi.nlm.nih.gov/pubmed/1841726
http://dx.doi.org/10.1105/tpc.3.7.719
 
[22]Hua, S.B., Dube, S.K., Barnett, N.M. and Kung, S.D. (1991) Nucleotide Sequence of Gene oee2-A and Its cDNA Encoding 23 kDa Polypeptide of the Oxygen-Evolving Complex of Photosystem II in Tobacco. Plant Molecular Biology, 17, 551-553. http://www.ncbi.nlm.nih.gov/pubmed/1884009
http://dx.doi.org/10.1007/BF00040655
 
[23]Hua, S.B., Dube, S.K. and Kung, S.D. (1995) Organ-Specific Expression of the Nuclear Gene Encoding OEE2 of Photosystem-II Oxygen-Evolving Complex in Nicotiana tabacum. Journal of Plant Biochemistry and Biotechnology, 4, 109-111. http://dx.doi.org/10.1007/BF03262964
 
[24]Hurley, J.H. (1996) The Sugar Kinase Heat Shock Protein 70 Actin Super Family: Implications of Conserved Structure for Mechanism. Annual Review of Biophysics and Biomolecular Structure, 25, 137-162. http://www.ncbi.nlm.nih.gov/pubmed/8800467
 
[25]Park, C.J., Kim, K.J., Shin, R., Park, J.M., Shin, Y.C. and Paek, K.H. (2004) Pathogenesis-Related Protein 10 Isolated from Hot Pepper Functions as a Ribonuclease in an Antiviral Pathway. Plant Journal, 37, 186-198. http://www.ncbi.nlm.nih.gov/pubmed/14690503
http://dx.doi.org/10.1046/j.1365-313X.2003.01951.x                                                               eww150119lx
标签：

评论

发表评论

此博客中的热门博文

Does Immigration Promote the Investment of the Monopolistic Firm?

In the present paper, we examine the effect of increasing uncertainty of immigrants’ growth on the optimal timing of investment of a firm that has a monopolistic power over the labor market. It is revealed that when the uncertainty of immigrants’ growth is more than a threshold level, increasing uncertainty of immigrants’ growth accelerates the optimal timing of firms’ investment and enhances the economic growth, even if the uncertainty of immigrants’ growth is formulated by the geometric Brownian motion, which is in sharp contrast to the standard result that an increase in the uncertainty postpones the optimal timing. With an increase in the immigrants over the past ten years, workforces in the host countries have been growing significantly to the extent that the immigrants represent 70% of the increase in the workforce in Europe, and 47% in the United States as OECD indicates. In the present paper, we attempted to investigate the effect of increased uncertainty caused by the growi...
发表评论
阅读全文

Education Policy Implementation: A Mechanism for Enhancing Primary Education Development in Zanzibar

Education is one of the fundamental rights of individuals; therefore, the government of a country needs to develop and strengthen educational policy and quality as well as to ensure that everyone has equal access to basic education. The improvement of access and quality of education in the world is becoming as an essential factor in development, whereas the basic education (primary school), is acknowledged as a foundation of the higher educational development for every country. To fulfill this goal, governments introduce several policies and procedures; however, it requires some reforms and participation from the politician, policymakers, and other stakeholders to re-examine educational policy so that it can lead to multiplication and betterment of the reforms. Educational reforms actually focus on accountability. A positive educational development and reform is very challenging and needs more effort and strategy on how to use and utilize the resources effectively as such it can achie...
发表评论
阅读全文