跳至主要内容

Proteins from Punctularia atropurpurascens with Biotechnological Applications

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52638#.VJzIncCAM4

Basidiomycetes are able to biodegrade waste and xenobiotic molecules through the production of extracellular enzymes. For example, white-rot fungi produce lignin-degrading enzymes which are capable of efficiently decolorizing dye solutions. Many mushrooms also produce lectins, a group of proteins which bind specifically to the carbohydrates in glycoconjugates. Several fungal lectins target their specificities towards oligosaccharides present in mammalian glycoproteins, thus constituting excellent ligands for the preparation of affinity adsorbents useful in isolation and characterization of these glycoproteins. In this study we isolated and characterized two different proteins, a lectin and a laccase, present in extracts from Punctularia atropurpurascens. The lectin isolated from the mycelium extract, was immobilized on activated-Sepharose and used to evaluate the interaction with three glycoproteins. The adsorbent was able to efficiently adsorb and elute bovine lactoferrin, constituting a promising tool for the purification of this glycoprotein. In vitro experiments revealed that the lectin also exhibited antimicrobial activity against Aspergillus niger. Laccase activity was detected in the extracellular extract from P. atropurpurascens. This enzyme, in both soluble and immobilized forms, was able to degrade Remazol Brilliant Blue R and Acid Blue 25 dyes. The biological activities found in this fungus demonstrate its potential for various biotechnological applications.
Cite this paper
Alborés, S. , Bustamante, M. , Fraguas, L. and Cerdeiras, M. (2014) Proteins from Punctularia atropurpurascens with Biotechnological Applications. Natural Resources, 5, 915-925. doi: 10.4236/nr.2014.515078. 
 

[1] Eichlerova, I., Homolka, L., Lisa, L. and Nerud, F. (2005) Orange G and Remazol Brilliant Blue R Decolorization by White Rot Fungi Dichomitus squalens, Ischnoderma resinosum and Pleurotus calyptratus. Chemosphere, 60, 398-404.
http://dx.doi.org/10.1016/j.chemosphere.2004.12.036
[2] Zeng, X., Cai, Y., Liao, X., Zeng, X., Luo, S. and Zhang, D. (2012) Anthraquinone Dye Assisted the Decolorization of Azo Dyes by a Novel Trametes trogii Laccase. Process Biochemistry, 47, 160-163.
http://dx.doi.org/10.1016/j.procbio.2011.10.019
[3] Koyani, R.D., Sanghvi, G.V., Sharma, R.K. and Rajput, K.S. (2013) Contribution of Lignin Degrading Enzymes in Decolourisation and Degradation of Reactive Textile Dyes. International Biodeterioration & Biodegradation, 77, 1-9.
http://dx.doi.org/10.1016/j.ibiod.2012.10.006
[4] Wong, J.H., Ng, T.B., Cheung, R.C.F., Ye, X.J., Wang, H.X., Lam, S.K., Lin, P., Chan, Y.S., Fang, E.F., Ngai, P.H.K., Xia, L.X., Ye, X.Y., Jiang, Y. and Liu, F. (2010) Proteins with Antifungal Properties and Other Medicinal Applications from Plants and Mushrooms. Applied Microbiology and Biotechnology, 87, 1221-1235.
http://dx.doi.org/10.1007/s00253-010-2690-4
[5] Sharon, N. (2008) Lectins: Past, Present and Future. Biochemical Society Transactions, 36, 1457-1460.
http://dx.doi.org/10.1042/BST0361457
[6] Marty-Detraves, C., Francis, F., Baricault, L., Fournier, D. and Paquereau, L. (2004) Inhibitory Action of a New Lectin from Xerocomus chrysenteron on Cell-Substrate Adhesion. Molecular and Cellular Biochemistry, 258, 49-55.
http://dx.doi.org/10.1023/B:MCBI.0000012835.08001.8f
[7] Kilpatrick, D.C. (2000) Mannan-Binding Lectin Concentration during Normal Human Pregnancy. Human Reproduction, 15, 941-943.
http://dx.doi.org/10.1093/humrep/15.4.941
[8] Liu, Q., Ng, T. and Wang, H. (2013) Isolation and Characterization of a Novel Lectin from the Wild Mushroom Oudemansiella radicata (Relhan.: Fr.) Sing. Biotechnology and Bioprocess Engineering, 18, 465-471.
http://dx.doi.org/10.1007/s12257-012-0699-5
[9] Chumkhunthod, P., Rodtong, S., Lambert, S.J., Fordham-Skelton, A.P., Rizkallah, P.J., Wilkinson, M.C. and Reynolds, C.D. (2006) Purification and Characterization of an N-Acetyl-D-Galactosamine-Specific Lectin from the Edible Mushroom Schizophyllum commune. Biochimica et Biophysica Acta—General Subjects, 1760, 326-332.
http://dx.doi.org/10.1016/j.bbagen.2006.01.015
[10] Jung, E.C., Kim, K.D., Bae, C.H., Kim, J.C., Kim, D.K. and Kim, H.H. (2007) A Mushroom Lectin from Ascomycete Cordyceps militaris. Biochimica et Biophysica Acta—General Subjects, 1770, 833-838.
http://dx.doi.org/10.1016/j.bbagen.2007.01.005
[11] She, Q.B., Ng, T.B. and Liu, W.K. (1998) A Novel Lectin with Potent Immunomodulatory Activity Isolated from Both Fruiting Bodies and Cultured Mycelia of the Edible Mushroom Volvariella volvacea. Biochemical and Biophysical Research Communications, 247, 106-111.
|http://dx.doi.org/10.1006/bbrc.1998.8744
[12] Wang, H.X., Ng, T.B., Liu, W.K., Ooi, V.E.C. and Chang, S.T. (1995) Isolation and Characterization of Two Distinct Lectins with Antiproliferative Activity from the Cultured Mycelium of the Edible Mushroom Tricholoma mongolicum. International Journal of Peptide and Protein Research, 46, 508-513.
http://dx.doi.org/10.1111/j.1399-3011.1995.tb01606.x
[13] Sharon, N. and Lis, H. (2007) Lectins. 2nd Edition, Springer, The Netherlands, 454.
[14] Bavendam, W. (1928) Uber das Vorkommen den Nachweis von Oxydasen beiholzzerstorenden. Pilzen Z. Pflanzenkrank. Pflanzenschutz, 38, 257-276.
[15] Borokhov, O. and Rothenburger, S. (2000) Rapid Dye Decolorization Method for Screening Potential Wood Preservatives. Applied and Environmental Microbiology, 66, 5457-5459.
http://dx.doi.org/10.1128/AEM.66.12.5457-5459.2000
[16] Alborés, S., Mora, P., Cerdeiras, M.P. and Fraguas, L.F. (2013) Screening for Lectins from Basidiomycetes and Isolation of Punctularia atropurpurascens Lectin. Journal of Basic Microbiology, 54, 89-96.
http://dx.doi.org/10.1002/jobm.201200229
[17] Galliano, H., Gas, G., Seris, J.L. and Boudet, A.M. (1991) Lignin Degradation by Rigidoporus lignosus Involves Synergistic Action of Two Oxidizing Enzymes: Mn Peroxidase and Laccase. Enzyme and Microbial Technology, 13, 478482.
http://dx.doi.org/10.1016/0141-0229(91)90005-U
[18] Castillo, M.D.P., Stenstrom, J. and Ander, P. (1994) Determination of Manganese Peroxidase Activity with 3-Methyl2-Benzothiazolinone Hydrazone and 3-(Dimethylamino)Benzoic Acid. Analytical Biochemistry, 218, 399-404.
http://dx.doi.org/10.1006/abio.1994.1198
[19] Tien, M. and Kirk, T.K. (1988) Lignin Peroxidase of Phanerochaete chrysosporium. In: Wood, W.A. and Kellogg, S.T., Eds., Methods in Enzymology-Biomass, Part B, Lignin, Pectin, and Chitin, Vol. 161, Academic Press, Inc., San Diego, 238-249.
[20] Giacomini, C., Villarino, A., Franco-Fraguas, L. and Batista-Viera, F. (1998) Immobilization of [Beta]-Galactosidase from Kluyveromyces lactis on Silica and Agarose: Comparison of Different Methods. Journal of Molecular Catalysis B: Enzymatic, 4, 313-327.
http://dx.doi.org/10.1016/S1381-1177(98)00071-X
[21] Rahalison, L., Hamburger, M., Hostettman, K., Monod, M. and Frenck, E. (1991) A Bioautographic Agar Overlay Method for the Detection of Antifungal Compounds from Higher Plants. Phytochemical Analysis, 2, 199-203.
http://dx.doi.org/10.1002/pca.2800020503
[22] Wan, C. and Li, Y. (2012) Fungal Pretreatment of Lignocellulosic Biomass. Biotechnology Advances, 30, 1447-1457.
http://dx.doi.org/10.1016/j.biotechadv.2012.03.003
[23] Srinivasan, C., D’Souza, T.M., Boominathan, K. and Reddy, C.A. (1995) Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767. Applied and Environmental Microbiology, 61, 4274-4277.
[24] Wong, Y. and Yu, J. (1999) Laccase-Catalyzed Decolorization of Synthetic Dyes. Water Research, 33, 3512-3520.
http://dx.doi.org/10.1016/S0043-1354(99)00066-4
[25] Cai, Y., Wu, H., Liao, X., Ding, Y., Sun, J. and Zhang, D. (2010) Purification and Characterization of Novel Manganese Peroxidase from Rhizoctonia sp. SYBC-M3. Biotechnology and Bioprocess Engineering, 15, 1016-1021.
http://dx.doi.org/10.1007/s12257-010-0130-z
[26] Baldrian, P. (2006) Fungal Laccases—Occurrence and Properties. FEMS Microbiology Reviews, 30, 215-242.
http://dx.doi.org/10.1111/j.1574-4976.2005.00010.x
[27] Ben Younes, S. and Sayadi, S. (2011) Purification and Characterization of a Novel Trimeric and Thermotolerant Laccase Produced from the Ascomycete Scytalidium thermophilum Strain. Journal of Molecular Catalysis B: Enzymatic, 73, 35-42.
http://dx.doi.org/10.1016/j.molcatb.2011.07.014
[28] Raghukumar, C., D’Souza, T.M., Thorn, R.G. and Reddy, C.A. (1999) Lignin-Modifying Enzymes of Flavodon flavus, a Basidiomycete Isolated from a Coastal Marine Environment. Applied and Environmental Microbiology, 65, 21032111.
[29] Fukushima, Y. and Kirk, T.K. (1995) Laccase Component of the Ceriporiopsis subvermispora Lignin-Degrading System. Applied and Environmental Microbiology, 61, 872-876.
[30] Vaithanomsat, P., Apiwatanapiwat, W., Petchoy, O. and Chedchant, J. (2010) Production of Ligninolytic Enzymes by White-Rot Fungus Datronia sp. KAPI0039 and Their Application for Reactive Dye Removal. International Journal of Chemical Engineering.
http://dx.doi.org/10.1155/2010/162504
[31] Kornillowicz-Kowalska, T. and Rybczynska, K. (2012) Decolorization of Remazol Brilliant Blue (RBBR) and Poly R-478 Dyes by Bjerkandera adusta CCBAS 930. Central European Journal of Biology, 7, 948-956.
http://dx.doi.org/10.2478/s11535-012-0076-6
[32] Kim, H., Lee, S., Ryu, S. and Choi, H.T. (2012) Decolorization of Remazol Brilliant Blue R by a Purified Laccase of Polyporus brumalis. Applied Biochemistry and Biotechnology, 166, 159-164.
http://dx.doi.org/10.1007/s12010-011-9412-y
[33] Bibi, I. and Bhatti, H.N. (2012) Enhanced Biodecolorization of Reactive Dyes by Basidiomycetes under Static Conditions. Applied Biochemistry and Biotechnology, 166, 2078-2090.
http://dx.doi.org/10.1007/s12010-012-9635-6
[34] Wesenberg, D., Kyriakides, I. and Agathos, S.N. (2003) White-Rot Fungi and Their Enzymes for the Treatment of Industrial Dye Effluents. Biotechnology Advances, 22, 161-187.
http://dx.doi.org/10.1016/j.biotechadv.2003.08.011
[35] Nunes, E., de Souza, M.A., Vaz, A.F., Santana, G.M., Gomes, F., Coelho, L.C., Paiva, P.M., da Silva, R.M., SilvaLucca, R., Oliva, M.L., Guarnieri, M. and Correia, M.T. (2011) Purification of a Lectin with Antibacterial Activity from Bothrops leucurus Snake Venom. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 159, 57-63.
http://dx.doi.org/10.1016/j.cbpb.2011.02.001
[36] Paiva, P.M.G., Gomes, F.S., Napoleao, T.H., Sá, R.A., Correia, M.T.S. and Coelho, L.C.B. (2010) Antimicrobial Activity of Secondary Metabolites and Lectins from Plants. In: Méndez Vilas, A., Ed., Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 396-406.                              eww141226lx
标签：

评论

发表评论

此博客中的热门博文

Does Immigration Promote the Investment of the Monopolistic Firm?

In the present paper, we examine the effect of increasing uncertainty of immigrants’ growth on the optimal timing of investment of a firm that has a monopolistic power over the labor market. It is revealed that when the uncertainty of immigrants’ growth is more than a threshold level, increasing uncertainty of immigrants’ growth accelerates the optimal timing of firms’ investment and enhances the economic growth, even if the uncertainty of immigrants’ growth is formulated by the geometric Brownian motion, which is in sharp contrast to the standard result that an increase in the uncertainty postpones the optimal timing. With an increase in the immigrants over the past ten years, workforces in the host countries have been growing significantly to the extent that the immigrants represent 70% of the increase in the workforce in Europe, and 47% in the United States as OECD indicates. In the present paper, we attempted to investigate the effect of increased uncertainty caused by the growi...
发表评论
阅读全文

Education Policy Implementation: A Mechanism for Enhancing Primary Education Development in Zanzibar

Education is one of the fundamental rights of individuals; therefore, the government of a country needs to develop and strengthen educational policy and quality as well as to ensure that everyone has equal access to basic education. The improvement of access and quality of education in the world is becoming as an essential factor in development, whereas the basic education (primary school), is acknowledged as a foundation of the higher educational development for every country. To fulfill this goal, governments introduce several policies and procedures; however, it requires some reforms and participation from the politician, policymakers, and other stakeholders to re-examine educational policy so that it can lead to multiplication and betterment of the reforms. Educational reforms actually focus on accountability. A positive educational development and reform is very challenging and needs more effort and strategy on how to use and utilize the resources effectively as such it can achie...
发表评论
阅读全文