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Production of Manganese Peroxidase by Trametes villosa on Unexpensive Substrate and Its Application in the Removal of Lignin from Agricultural Wastes

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Manganese peroxidase (MnP) is a ligninolytic enzyme that is involved in the removal of lignin from the cell wall of plants. This removal facilitates the access of hydrolytic enzymes to the carbohydrate polymers that are hydrolyzed to simple sugars, which allows the subsequent fermentation to obtain bioproducts, such as ethanol. In this work, response surface methodology (RSM) was employed to optimize the culture conditions on unexpensive substrate for MnP secretion by Trametes villosa. Three independent variables were evaluated (i.e., temperature, moisture content and pH). The crude extract containing MnP was used in the delignification experiment and it caused a reduction in lignin content for all residues tested: 35.05 ± 1.45 (%) for the sugar cane bagasse; 63.11 ± 0.06 (%) for the sisal fiber and 39.61 ± 0.39 (%) for the coconut shell, under the reaction conditions tested after 4 hours of fermentation. The preliminary results exhibited the potential application of this enzyme in the removal of lignin from plant residues. However, the conditions should be evaluated and optimized for each residue type.
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Silva, M. , Souza, V. , Santos, V. , Kamida, H. , Vasconcellos-Neto, J. , Góes-Neto, A. and Bello Koblitz, M. (2014) Production of Manganese Peroxidase by Trametes villosa on Unexpensive Substrate and Its Application in the Removal of Lignin from Agricultural Wastes. Advances in Bioscience and Biotechnology, 5, 1067-1077. doi: 10.4236/abb.2014.514122. 
 

[1] Singh, R., Ashish S., Sapna, T. and Monika, S. (2014) A Review on Delignification of Lignocellulosic Biomass for Enhancement of Ethanol Production Potential. Renewable and Sustainable Energy Reviews, 32, 713-728.
http://dx.doi.org/10.1016/j.rser.2014.01.051
[2] Chang, A.J., Fan, J.Y. and Wen, X.H. (2012) Screening of Fungi Capable of Highly Selective Degradation of Lignin in Rice Straw. International Biodeterioration & Biodegradation, 72, 26-30.
http://dx.doi.org/10.1016/j.ibiod.2012.04.013
[3] Hofrichter, M. (2002) Review: Lignin Conversion by Manganese Peroxidase (MnP). Enzyme and Microbial Technology, 30, 454-466.
http://dx.doi.org/10.1016/S0141-0229(01)00528-2
[4] Majumdar, S., Tiit, L.K., Jose, O.S., Stefan, B., Satish, K.N., John, E.C. and John, A.G. (2014) Roles of Small Laccases from Streptomyces in Lignin Degradation. Biochemistry, 53, 4047-4058.
http://dx.doi.org/10.1021/bi500285t
[5] Fujii, K., Mari, U., Chie, H., Shinya, F. and Takashi, K. (2013) Environmental Control of Lignin Peroxidase, Manganese Peroxidase, and Laccase Activities in Forest Floor Layers in Humid Asia. Soil Biology and Biochemistry, 57, 109-115.
http://dx.doi.org/10.1016/j.soilbio.2012.07.007
[6] Knezevic, A., Ivan, M., Mirjana, S. and Jelena, V. (2013) Potential of Trametes Species to Degrade Lignin. International Biodeterioration & Biodegradation, 85, 52-56.
http://dx.doi.org/10.1016/j.ibiod.2013.06.017
[7] Elisashvili, V. and Kachlishvili, V. (2009) Physiological Regulation of Laccase and Manganese Peroxidase Production by White-Rot Basidiomycetes. Journal of Biotechnology, 144, 37-42.
http://dx.doi.org/10.1016/j.jbiotec.2009.06.020
[8] Hiscox, J., Baldrian, P., Rogers, H. and Boddy, L. (2010) Changes in Oxidative Enzyme Activity during Interspecific Mycelial Interactions Involving the White-Rot Fungus Trametes versicolor. Fungal Genetic and Biology, 47, 562-571.
http://dx.doi.org/10.1016/j.fgb.2010.03.007
[9] Tuomela, M., Vikman, M., Hatakka, A. and Itavaara, M. (2000) Biodegradation of Lignin in Compost Environment: A Review. Bioresource Technology, 72, 169-183.
http://dx.doi.org/10.1016/S0960-8524(99)00104-2
[10] Sun, X., Zhang, R. and Zhang, Y. (2004) Production of Lignocellulolytic Enzymes by Trametes gallica and Detection of Polysaccharide Hydrolase and Laccase Activities in Polyacrylamide Gels. Jounal of Basic Microbiology, 44, 220-231.
http://dx.doi.org/10.1002/jobm.200310376
[11] Songulashvili, G., Elisashvili, V., Wasser, S.P., Nevo, E. and Hadar, Y. (2007) Basidiomycetes Laccase and Manganese Peroxidase Activity in Submerged Fermentation of Food Industry Wastes. Enzyme and Microbial Technology, 41, 57-61.
http://dx.doi.org/10.1016/j.enzmictec.2006.11.024
[12] Irbe, I., Elisashvili, V., Asatiani, M.D., Janberga, A., Andersone, I., Andersons, B., Biziks, V. and Grinins, J. (2014) Lignocellulolytic Activity of Coniophora puteana and Trametes versicolor in Fermentation of Wheat Bran and Decay of Hydrothermally Modified Hardwoods. International Biodeterioration & Biodegradation, 86, 71-78.
http://dx.doi.org/10.1016/j.ibiod.2013.06.027
[13] Arantes, V., Silva, E.M. and Milagres, A.M.F. (2011) Optimal Recovery Process Conditions for Manganese Peroxidase Obtained by Solid-State Fermentation of Eucalyptus Residue Using Lentinula edodes. Biomass and Bioenergy, 35, 4040-4044.
http://dx.doi.org/10.1016/j.biombioe.2011.06.042
[14] Lopes, M.A., Gomes, D.S., Koblitz, M.G.B., Pirovani, C.P, Cascardo, J.C.M., Góes-Neto, A. and Micheli, F. (2008) Use of Response Surface Methodology to Examine Chitinase Regulation in the Basidiomycete Moniliophthora perniciosa. Mycological Research, 112, 399-406.
http://dx.doi.org/10.1016/j.mycres.2007.10.017
[15] Levin, L., Herrmann, C. and Papinutti, V.L. (2008) Optimization of Lignocellulolytic Enzyme Production by the White-Rot Fungus Trametes trogii in Solid-State Fermentation Using Response Surface Methodology. Biochemical Engineering Journal, 39, 207-214.
http://dx.doi.org/10.1016/j.bej.2007.09.004
[16] Raol, G.G., Prajapati, V.S. and Raol, B.V. (2014) Formulation of Low-Cost, Lactose-Free Production Medium by Response Surface Methodology for the Production of β-Galactosidase Using Halotolerant Aspergillus tubengensis GR-1. Biocatalysis and Agricultural Biotechnology.
[17] Qiu, J., Song, F., Qiu, Y., Li, X. and Guan, X. (2013) Optimization of the Medium Composition of a Biphasic Production System for Mycelial Growth and Spore Production of Aschersonia placenta Using Response Surface Methodology. Journal of Invertebrate Pathology, 112, 108-115.
http://dx.doi.org/10.1016/j.jip.2012.10.010
[18] Palmieri, G., Cennamo, G. and Sannia, G. (2005) Remazol Brilliant Blue R Decolourisation by the Fungus Pleurotus ostreatus and Its Oxidative Enzymatic System. Enzyme and Microbial Technology, 36, 17-24.
[19] Uetanabaro, A.P.T. and Góes-Neto, A. (2006) Importance of Culture Collections of Microorganisms (CCMs) for the Conservation of Microbial Biotechnological Resources of the Brazilian Semi-Arid Region. In: Queiroz, L.P., Rapini, A. and Giulietti, A.M., Eds., Towards Greater Knowledge of the Brazilian Semi-Arid Biodiversity, 1st Edition, Ministério da Ciência e Tecnologia, Brasília-DF, 41-43.
[20] Drechsler-Santos, E.R., Gibertoni, T.B., Góes-Neto, A. and Cavalcanti, M.A.Q. (2009) A Re-Evalutation of the Lignocellulolytic Agaricomycetes from the Brazilian Semi-Arid Region. Mycotaxon, 108, 241-244.
http://dx.doi.org/10.5248/108.241
[21] Kuwahara, M., Glenn, J.K., Morgan, M.N. and Gold, M.H. (1984) Separation and Characterization of Two Extracellular H2O2-Dependent Oxidases from Ligninolytic Cultures of Phanerochaete chrysosporium. FEBS Letters, 169, 247-250.
http://dx.doi.org/10.1016/0014-5793(84)80327-0
[22] Menezes, C.R., Silva, I.S. and Durrant, L.R. (2009) Bagaço de cana: Fonte para produção de enzimas ligninocelulolíticas. Estudos Tecnológicos, 5, 68-78.
http://dx.doi.org/10.4013/ete.2009.51.05
[23] Murthy, M.V.R., Padmanbham, S., Ramakrishna, M. and Lonsane, B.K. (1997) Comparison of Nine Different Caseinolytic Assays for Estimation of Proteinase Activity and Further Improvement of the Best Method. Food Biotechnology, 11, 1-23.
http://dx.doi.org/10.1080/08905439709549919
[24] Van Soest, P.J. (1994) Nutritional Ecology of the Ruminant. Cornell University Press, Ithaca.
[25] Cupul, W.C., Abarca, G.H., Carrera, D.M. and Vázquez, R.R. (2014) Enhancement of Ligninolytic Enzyme Activities in a Trametes maxima-Paecilomyces carneus Co-Culture: Key Factors Revealed after Screening Using a Plackett-Burman Experimental Design. Electronic Journal of Biotechnology, 17, 114-121.
http://dx.doi.org/10.1016/j.ejbt.2014.04.007
[26] Fernández-Fueyo, E., Castanera, R., Ruiz-Dueñas, F.J., López-Lucendo, M.F., Ramírez, L., Pisabarro, A.G. and Martínez, A.T. (2014) Ligninolytic Peroxidase Gene Expression by Pleurotus ostreatus: Differential Regulation in Lignocellulose Medium and Effect of Temperature and pH. Fungal Genetics and Biology, 72, 150-161.
http://dx.doi.org/10.1016/j.fgb.2014.02.003
[27] Fink-Boots, M., Malarczyk, E. and Leonowicz, A. (1999) Increased Enzymatic Activities and Levels of Superoxide Anion and Phenolic Compounds in Cultures of Basidiomycetes after Temperature Stress. Acta Biotechnology, 19, 319-330.
http://dx.doi.org/10.1002/abio.370190407
[28] Elisashvili, V., Torok, T., Kachlishvili, E., Khardziani, T. and Metreveli, E. (2011) Evaluation and Regulation of the Lignocellulolytic Activity of Novel White-Rot Basidiomycetes. Global Journal of Biochemistry, 2, 134-141.
[29] Jaszek, M., Grzywnowicz, K., Malarczyk, E. and Leonowicz, A. (2006) Enhanced Extracellular Laccase Activity as a Part of the Response System of White Rot Fungi Trametes versicolor and Abortiporus biennis to Paraquat-Caused Oxidative Stress Conditions. Pesticide Biochemistry and Physiology, 85, 147-154.
http://dx.doi.org/10.1016/j.pestbp.2006.01.002
[30] Wang, F., Hu, J., Guo, C. and Liu, C. (2014) Enhanced Laccase Production by Trametes versicolor Using Corn Steep Liquor as Both Nitrogen Source and Inducer. Bioresource Technology, 166, 602-605.
http://dx.doi.org/10.1016/j.biortech.2014.05.068
[31] Carabajal, M., Harald, K., Laura, L., Nico, J., Martin, H. and René, U. (2013) The Secretome of Trametes versicolor Grown on Tomato Juice Medium and Purification of the Secreted Oxidoreductases Including a Versatile Peroxidase. Journal of Biotechnology, 168, 15-23.
http://dx.doi.org/10.1016/j.jbiotec.2013.08.007
[32] Baborová, P., Möder, M., Baldrian, P., Cajthamlová, K. and Cajthaml, T. (2006) Purification of a New Manganese Peroxidase of the White-Rot Fungus Irpex lacteus, and Degradation of Polycyclic Aromatic Hydrocarbons by the Enzyme. Research in Microbiology, 157, 248-253.
http://dx.doi.org/10.1016/j.resmic.2005.09.001
[33] Machado, K.M.G., Matheus, R.D. and Bononi, V.L.R. (2005) Ligninolytic Enzymes Production and Remazol Brilliant Blue R Decolorization by Tropical Brazilian Basidiomycetes Fungi. Brazilian Journal of Microbiology, 36, 246-252.
http://dx.doi.org/10.1590/S1517-83822005000300008
[34] Kapich, A.N., Prior, B.A., Botha, A., Galkin, S., Lundell, T. and Hatakka, A. (2004) Effect of Lignocellulose-Containing Substrates on Production of Ligninolytic Peroxidases in Submerged Cultures of Phanerochaete chrysosporium ME-446. Enzyme and Microbial Technology, 34, 187-195.
http://dx.doi.org/10.1016/j.enzmictec.2003.10.004
[35] Kapich, A.N., Steffen, K., Hofrichter, M. and Hatakka, A. (2005) Involvement of Lipid Peroxidation in the Degradation of a Non-Phenolic Lignin Model Compound by Manganese Peroxidase of the Litter-Decomposing Fungus Stropharia coronilla. Biochemical and Biophysical Research Communications, 330, 371-377.
http://dx.doi.org/10.1016/j.bbrc.2005.02.167
[36] Lopez, M.J., Vargas-García, M.C., Suárezestrella, F., Nichols, N.N., Dien, B.S. and Moreno, J. (2007) Lignocellulose-Degrading Enzymes Produced by the Ascomycete Coniochaeta ligniaria and Related Species: Application for a Lignocellulosic Substrate Treatment. Enzyme and Microbial Technology, 40, 794-800.
http://dx.doi.org/10.1016/j.enzmictec.2006.06.012
[37] Yamanaka, R., Soares, C.F., Matheus, D.R. and Machad, K.M.G. (2008) Lignolytic Enzymes Produced by Trametes villosa CCB176 under Different Culture Conditions. Bra-zilian Journal of Microbiology, 39, 78-84.
http://dx.doi.org/10.1590/S1517-83822008000100019
[38] Elisashvili, V.V., Penninckx, M., Kachlishvili, E., Tsiklauri, N., Metreveli, E., Kharziani, T. and Kvesitadze, G. (2008) Lentinus edodes and Pleurotus Species Lignocellulolytic Enzymes Activity in Submerged and Solid-State Fermentation of Lignocellulosic Wastes of Different Composition. Bioresource Technology, 99, 457-462.
http://dx.doi.org/10.1016/j.biortech.2007.01.011
[39] Elisashvili, V., Parlar, H., Kachlishvili, E., Chichua, D. and Kvesitadze, G. (2001) Ligninolytic Activity of Basidiomycetes Grown under Submerged and Solid-State Fermentation on Plant Raw Material (Sawdust of Grapevine Cuttings). Advances in Food Science, 23, 117-123.
[40] Xu, F.J., Chen, H.Z. and Li, Z.H. (2001) Solid-State Production of Lignin Peroxidase (LiP) and Manganese Peroxidase (MnP) by Phanerochaete chrysosporium Using Steam-Exploded Straw as Substrate. Bioresource Technology, 80, 149-151.
http://dx.doi.org/10.1016/S0960-8524(01)00082-7
[41] Vassilev, N., Requena, A.R., Nieto, L.M., Nikolaeva, I. and Vassilev, M. (2009) Production of Manganese Peroxidase by Phanerochaete chrisosporium Grown on Medium Containing Agro-Wastes/Rock Phosphate and Biocontrol Properties of the Final Product. Industrial Crops and Products, 30, 28-32.
http://dx.doi.org/10.1016/j.indcrop.2009.01.001
[42] Papinutti, V.L. and Forchiassin, F. (2007) Lignocellulolytic Enzymes from Fomes sclerodermeus Growing in Solid-State Fermentation. Journal of Food Engineering, 81, 54-59.
http://dx.doi.org/10.1016/j.jfoodeng.2006.10.006
[43] Wan, C. and Li, Y. (2012) Fungal Pre-treatment of Lignocellulosic Biomass. Biotechnology Advances, 30, 1447-1457.
http://dx.doi.org/10.1016/j.biotechadv.2012.03.003
[44] Arora, D.S., Chander, M. and Gill, P.K. (2002) Involvement of Lignin Peroxidase, Manganese Peroxidase and Laccase in Degradation and Selective Ligninolysis of Wheat Straw. International Biodeterioration & Biodegradation, 50, 115-120.
http://dx.doi.org/10.1016/S0964-8305(02)00064-1
[45] Deswal, D., Gupta, R. Nandal, P. and Kuhad, R.C. (2013) Fungal Pretreatment Improves Amenability of Lignocellulosic Material for Its Saccharification to Sugars. Carbohydrate Polymers, 99, 264-269.
http://dx.doi.org/10.1016/j.carbpol.2013.08.045
[46] Moniruzzaman, M. and Ono, T. (2012) Ionic Liquid Assisted Enzymatic Delignification of Wood Biomass: A New “Green” and Efficient Approach for Isolating of Cellulose Fibers. Biochemical Engineering Journal, 60, 156-160.
http://dx.doi.org/10.1016/j.bej.2011.11.001
[47] Mukhopadhyay, M., Kuila, A., Tuli, D.K. and Banerjee, R. (2011) Enzymatic Depolymerization of Ricinus communis, a Potential Lignocellulosic for Improved Saccharification. Biomass Bioenergy, 35, 3584-3591.
http://dx.doi.org/10.1016/j.biombioe.2011.05.013           eww141225lx
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