跳至主要内容

Derivation Method for Determining Sorbitol in Fruit Trees

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51833#.VHfVZmfHRK0

The goal of this study is to describe a methodology for the analysis of D-sorbitol using benzyl alcohol as derivatizing agent and liquid chromatography equipped with a diode-array detector (HPLC-DAD). The study was performed at the University of São Paulo State (UNESP), Ilha Solteira Campus, Brazil. The axillary buds of fruit trees were collected from the UNESP Teaching and Research Farm. Benzyl alcohol was used as derivatizing agent to extract D-sorbitol from the buds. Next, the D-sorbitol underwent solid phase extraction (SPE) using C18 cartridges and was then analyzed by liquid chromatography (HPLC-DAD). The HPLC-DAD derivatization method proposed showed excellent chromatography resolution and high accuracy in the separation of D-sorbitol (derivatized) for the axillary buds of avocado, black mulberry, Japanese pear and peach and also enabled eliminating the use of hazardous derivatizing agents.
Cite this paper
Canesin, R. , Isique, W. , Buzetti, S. and Souza, J. (2014) Derivation Method for Determining Sorbitol in Fruit Trees. American Journal of Plant Sciences, 5, 3457-3463. doi: 10.4236/ajps.2014.523361. 
 

[1] Stoop, J.M.H., Williamson, J.D. and Pharr, M. (1996) Manitol Metabolism in Plants: A Method for Coping with Stress. Trends in Plant Science, 1, 139-144. http://dx.doi.org/10.1016/S1360-1385(96)80048-3
[2] Negm, F.B. and Loeschier, W.H. (1981) Characterization and Partial Purification of Aldose-6-Phosphate Reductase (Alditol-6-Phosphate:NADP 1-Oxidoreductase) from Apple Leaves. Plant Physiology, 67, 139-142.
http://dx.doi.org/10.1104/pp.67.1.139
[3] Noiraud, N., Maurousset, L. and Lemoine, R. (2001) Transport of Polyols in Higher Plants. Plant Physiology Biochemistry, 39, 717-728. http://dx.doi.org/10.1016/S0981-9428(01)01292-X
[4] Belitz, H.D., Grosch, W. and Schieberle, P. (2009) Fruits and Fruit Products. Food Chemistry, 34, 807-861.
[5] Ball, G.F.M. (1990) The Application of HPLC to the Determination of Low Molecular Weight Sugars and Polyhydric Alcohols in Foods: A Review. Food Chemistry, 35, 117-152. http://dx.doi.org/10.1016/0308-8146(90)90026-Z
[6] Miyagi, M., Yokoyama, H. and Hibi, T. (2007) Sugar Microanalysis by HPLC with Benzoylation: Improvement via Introduction of a C-8 Cartridge and a High Efficiency ODS Column. Journal of Chromatography B, 854, 286-290.
http://dx.doi.org/10.1016/j.jchromb.2007.04.044
[7] Oehlke, J., Brudel, M. and Blasig, I.E. (1994) Benzylation of Sugars, Polyols and Amino Acids in Biological Fluids for High-Performance Liquid Chromatographic Analysis. Journal Chromatography B: Biomedical Science Applied, 655, 105. http://dx.doi.org/10.1016/0378-4347(94)00067-0
[8] Ribeiro, F.A.L., Ferreira, M.M.C., Morano, S.C., Da Silva, L.R. and Schneider, R.P. (2008) Validation Spreadsheet: A New Tool for Estimating the Analytical Figures of Merit for the Validation of Univariate Methods. Quimica Nova, 31, 164-171. http://dx.doi.org/10.1590/S0100-40422008000100029
[9] Meyer, V.R. (1996) Theoretical Principles. In: Practical High-Performance Liquid Chromatography, John Wiley & Sons, Hoboken, 17-45.
[10] International Conference on Harmonisation (ICH) (1995) Guidance for Industry Q2A: Text on Validation of Analytical Procedures.
[11] International Conference on Harmonisation (ICH) (1996) Guidance for Industry Q2B: Text on Validation of Analytical Procedures.
[12] Danzer, K. and Currie, L.A. (1998) Guidelines for Calibration in Analytical Chemistry. Pure and Applied Chemistry, 70, 993.
[13] Podder, S., Choudhury, J. and Roy, S. (2007) Secondary Benzylation with Benzyl Alcohols Catalyzed by a High-Valent Heterobimetallic Ir-Sn Complex. Journal of Organic Chemistry, 72, 3129-3132.
http://dx.doi.org/10.1021/jo0625094
[14] Muir, J.G., Rose, R.Y., Rosella, O., Liels, K., Barrett, J.S., Shepherd, S.J. and Gibson, P.R. (2009) Measurement of Short-Chain Carbohydrates in Common Australian Vegetables and Fruits by High-Performance Liquid Chromatography (HPLC). Journal Agricultural and Food Chemistry, 57, 554-565. http://dx.doi.org/10.1021/jf802700e
[15] Oura, Y., Yamada, K., Shiratake, K. and Yamaki, S. (2000) Purification and Characterization of a NAD+-Dependent Sorbitol Dehydrogenase from Japanese Pear Fruit. Phytochemistry, 54, 567-572.
http://dx.doi.org/10.1016/S0031-9422(00)00158-8
[16] Wrolstad, R.E. and Shallenberger, R.S. (1981) Free Sugars and Sorbitol in Fruits a Complilation from the Literature. Journal of the Association of Official Analytical Chemists, 61, 91-103.                eww141128lx
标签：

评论

发表评论

此博客中的热门博文

Electron Spin and Proton Spin in the Hydrogen and Hydrogen-Like Atomic Systems

Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52202#.VIj7tMnQrzE Author(s) Stanisław Olszewski * Affiliation(s) Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland . ABSTRACT The mechanical angular momentum and magnetic moment of the electron and proton spin have been calculated semiclassically with the aid of the uncertainty principle for energy and time. The spin effects of both kinds of the elementary particles can be expressed in terms of similar formulae. The quantization of the spin motion has been done on the basis of the old quantum theory. It gives a quantum number n = 1/2 as the index of the spin state acceptable for both the electron and proton
发表评论
阅读全文

Remarks on the Complexity of Signed k-Domination on Graphs

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53574#.VMnXsCzQrzE Author(s)    Chuan-Min Lee 1 , Cheng-Chien Lo 1 , Rui-Xin Ye 2 , Xun Xu 2 , Xiao-Han Shi 2 , Jia-Ying Li 2 Affiliation(s) 1 Department of Computer and Communication Engineering, Ming Chuan University, The First American University in Asia, Taoyuan, Taiwan, Chinese Taipei . 2 Department of Electronic Information Engineering, Fuzhou University, Fuzhou, China . ABSTRACT This paper is motivated by the concept of the signed k-domination problem and dedicated to the complexity of the problem on graphs. For any fixed nonnegative integer k, we show that the signed k-domination problem is NP-complete for doubly chordal graphs. For strongly chordal graphs and distance-hereditary graphs, we show that the signed k-domination problem can be solved in polynomial time. We also show that the problem is linear-time solvable for trees, interval graphs, and chordal comparability graphs
发表评论
阅读全文

Dietary Fiber Content of Waterleaf (Talinum triangulare (Jacq.) Willd) Cultivated with Organic and Conventional Fertilization in Different SeasonsDietary Fiber Content of Waterleaf (Talinum triangulare (Jacq.) Willd) Cultivated with Organic and Conventional Fertilization in Different Seasons

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53985#.VN21HizQrzE Author(s)  Nuri Andarwulan 1,2 , Didah Nur Faridah 1,2 , Yolanda Sylvia Prabekti 1 , Harum Fadhilatunnur 1 , Leo Mualim 3 , Sandra Arifin Aziz 3 , Luis Cisneros-Zevallos 4   Affiliation(s) 1 Department of Food Science and Technology, Bogor Agricultural University, Bogor, Indonesia . 2 Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, Bogor Agricultural University, Bogor, Indonesia . 3 Department of Agronomy and Horticulture, Bogor Agricultural University, Bogor, Indonesia . 4 Department of Horticultural Sciences, Texas A&M University, College Station, USA . ABSTRACT Waterleaf ( Talinum triangulare (Jacq.) Willd has long been eaten in Indonesia as vegetable and the main parts consumed are leaves and young shoots. Waterleaf is sticky presumably due to its pectin content which is associated to dietary fiber. The dietary fiber which
发表评论
阅读全文