跳至主要内容

Derivation Method for Determining Sorbitol in Fruit Trees

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51833#.VHfVZmfHRK0

The goal of this study is to describe a methodology for the analysis of D-sorbitol using benzyl alcohol as derivatizing agent and liquid chromatography equipped with a diode-array detector (HPLC-DAD). The study was performed at the University of São Paulo State (UNESP), Ilha Solteira Campus, Brazil. The axillary buds of fruit trees were collected from the UNESP Teaching and Research Farm. Benzyl alcohol was used as derivatizing agent to extract D-sorbitol from the buds. Next, the D-sorbitol underwent solid phase extraction (SPE) using C18 cartridges and was then analyzed by liquid chromatography (HPLC-DAD). The HPLC-DAD derivatization method proposed showed excellent chromatography resolution and high accuracy in the separation of D-sorbitol (derivatized) for the axillary buds of avocado, black mulberry, Japanese pear and peach and also enabled eliminating the use of hazardous derivatizing agents.
Cite this paper
Canesin, R. , Isique, W. , Buzetti, S. and Souza, J. (2014) Derivation Method for Determining Sorbitol in Fruit Trees. American Journal of Plant Sciences, 5, 3457-3463. doi: 10.4236/ajps.2014.523361. 
 

[1] Stoop, J.M.H., Williamson, J.D. and Pharr, M. (1996) Manitol Metabolism in Plants: A Method for Coping with Stress. Trends in Plant Science, 1, 139-144. http://dx.doi.org/10.1016/S1360-1385(96)80048-3
[2] Negm, F.B. and Loeschier, W.H. (1981) Characterization and Partial Purification of Aldose-6-Phosphate Reductase (Alditol-6-Phosphate:NADP 1-Oxidoreductase) from Apple Leaves. Plant Physiology, 67, 139-142.
http://dx.doi.org/10.1104/pp.67.1.139
[3] Noiraud, N., Maurousset, L. and Lemoine, R. (2001) Transport of Polyols in Higher Plants. Plant Physiology Biochemistry, 39, 717-728. http://dx.doi.org/10.1016/S0981-9428(01)01292-X
[4] Belitz, H.D., Grosch, W. and Schieberle, P. (2009) Fruits and Fruit Products. Food Chemistry, 34, 807-861.
[5] Ball, G.F.M. (1990) The Application of HPLC to the Determination of Low Molecular Weight Sugars and Polyhydric Alcohols in Foods: A Review. Food Chemistry, 35, 117-152. http://dx.doi.org/10.1016/0308-8146(90)90026-Z
[6] Miyagi, M., Yokoyama, H. and Hibi, T. (2007) Sugar Microanalysis by HPLC with Benzoylation: Improvement via Introduction of a C-8 Cartridge and a High Efficiency ODS Column. Journal of Chromatography B, 854, 286-290.
http://dx.doi.org/10.1016/j.jchromb.2007.04.044
[7] Oehlke, J., Brudel, M. and Blasig, I.E. (1994) Benzylation of Sugars, Polyols and Amino Acids in Biological Fluids for High-Performance Liquid Chromatographic Analysis. Journal Chromatography B: Biomedical Science Applied, 655, 105. http://dx.doi.org/10.1016/0378-4347(94)00067-0
[8] Ribeiro, F.A.L., Ferreira, M.M.C., Morano, S.C., Da Silva, L.R. and Schneider, R.P. (2008) Validation Spreadsheet: A New Tool for Estimating the Analytical Figures of Merit for the Validation of Univariate Methods. Quimica Nova, 31, 164-171. http://dx.doi.org/10.1590/S0100-40422008000100029
[9] Meyer, V.R. (1996) Theoretical Principles. In: Practical High-Performance Liquid Chromatography, John Wiley & Sons, Hoboken, 17-45.
[10] International Conference on Harmonisation (ICH) (1995) Guidance for Industry Q2A: Text on Validation of Analytical Procedures.
[11] International Conference on Harmonisation (ICH) (1996) Guidance for Industry Q2B: Text on Validation of Analytical Procedures.
[12] Danzer, K. and Currie, L.A. (1998) Guidelines for Calibration in Analytical Chemistry. Pure and Applied Chemistry, 70, 993.
[13] Podder, S., Choudhury, J. and Roy, S. (2007) Secondary Benzylation with Benzyl Alcohols Catalyzed by a High-Valent Heterobimetallic Ir-Sn Complex. Journal of Organic Chemistry, 72, 3129-3132.
http://dx.doi.org/10.1021/jo0625094
[14] Muir, J.G., Rose, R.Y., Rosella, O., Liels, K., Barrett, J.S., Shepherd, S.J. and Gibson, P.R. (2009) Measurement of Short-Chain Carbohydrates in Common Australian Vegetables and Fruits by High-Performance Liquid Chromatography (HPLC). Journal Agricultural and Food Chemistry, 57, 554-565. http://dx.doi.org/10.1021/jf802700e
[15] Oura, Y., Yamada, K., Shiratake, K. and Yamaki, S. (2000) Purification and Characterization of a NAD+-Dependent Sorbitol Dehydrogenase from Japanese Pear Fruit. Phytochemistry, 54, 567-572.
http://dx.doi.org/10.1016/S0031-9422(00)00158-8
[16] Wrolstad, R.E. and Shallenberger, R.S. (1981) Free Sugars and Sorbitol in Fruits a Complilation from the Literature. Journal of the Association of Official Analytical Chemists, 61, 91-103.                eww141128lx
标签：

评论

发表评论

此博客中的热门博文

Incorporation of High-Altitude Balloon Experiment in High School Science Classrooms

High-altitude balloon is a balloon, filled usually with helium or hydrogen that ascends into an area called “near space” or stratosphere. The most common type of high-altitude balloons are weather balloons. Other purposes include use as a platform for experiments in the upper atmosphere. Modern balloons generally contain electronic equipment such as radio transmitters, cameras, or satellite navigation systems, such as GPS receivers. The mission of the High-Altitude Balloon Experiment (HABE) is to acquire supporting data, validate enabling technologies, and resolve critical acquisition, tracking, and pointing (ATP) and fire control issues in support of future space-based precision pointing experiments. The use of high-altitude balloons offers a relatively low-cost, low-vibration test platform, a recoverable and reusable payload, worldwide launch capability, and a 'near- space' emulation of the future space systems operational scenarios. More recently, several university...
1 条评论
阅读全文

The Influence of Heated Soil in Crop of “Tamaris” Tomato Plants on the Biological Activity of the Rhizosphere Soil

Tomato is a plant with high heat requirements and sensitive to cold weather and frost. The optimum temperature for the growth of tomato plants is between 21˚C and 27˚C during the day and between 17˚C and 21˚C at night. The soil temperature is also very important for plant growth. The optimum soil temperature for tomato cultivation should be within the range 15˚C - 18˚C. Besides, the proper development of the root system depends on the optimal temperature of the soil. A temperature below 14˚C reduces and inhibits the growth of the root system and encourages the development of fungal and bacterial diseases. In this study, the authors aimed to evaluate the effect of heated soil on the population of bacteria, fungi and nematodes inhabiting the soil of tomato cultivar “Tamaris” growing in peat and coconut substrates. The experiment was carried out in 12 treatments and in 3 replications (one slab was one replication). The soils were tested in two different types of containers: cylinders...
发表评论
阅读全文

Effect of Proline Pretreatment on Grapevine Shoot-Tip Response to a Droplet-Vitrification Protocol

Proline is an α-amino acid that is used in the biosynthesis of proteins. Some studies have shown that proline has been accumulated in plants in response to biotic and abiotic stresses. Exogenous proline has thus been used for improving some plant cryopreservation protocols. Further enhancement of cryopreservation efficiency for  in vitro  grapevines could be expected if stresses linked to cryopreservation procedures could be reduced. In this study, the authors studied the possible beneficial effect of proline in grapevine cryopreservation. Single-node explants from  in vitro  grown grapevine plantlets ( Vitis vinifera  L. cv Portan) were cultured on shooting media (half-strength MS + 1 μM BAP) containing no proline (control) or 50, 500, or 2000 μM filter-sterilized L-proline. Shoot tips excised from these microshoots were subjected to a PVS2-based droplet-vitrification procedure. Control and rewarmed explants were grown on a recovery medium containing ...
发表评论
阅读全文