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Ustilaginoidea virens Infection of Rice in Arkansas: Toxicity of False Smut Galls, Their Extracts and the Ustiloxin Fraction

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http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50791#.VE8SIFfHRK0

Cool, wet conditions in the southern US during the maturing stages of rice in 1998 contributed to outbreaks of false smut caused by Ustilaginoidea virens. Water extracts of false smut galls in Asia have been reported to contain ustiloxin toxins, cyclic peptide antibiotics that interfered with microtubule function and caused “lupinosis”-like lesions in mice. Cell-free extracts from false smut galls on rice grown in Arkansas were fractionated by a published procedure for the purification of ustiloxins. The ustiloxin fraction was phytotoxic to Lemnapausicostata (duckweed) at ≥19 μg/ml, but the host plant, rice, was much less susceptible, exhibiting phytotoxic effects in germinating seeds at ≥1000 μg/ml. The aqueous extract of rice false smut galls showed no cytotoxicity to mammalian cell cultures at 200 μg/ml, but the ustiloxin fractionwas cytotoxic at 10 - 100 μg/ml. However, rice false smut galls were not toxic when fed to mice at 10% of chow, but caused feed refusal at higher concentrations. We conclude that for 1) the U. virens which causes false smut in southern USA differs from Asian isolates in that does not produce detectable ustiloxins; and 2) false smut affects the appearance, but not the food safety of rice in the United States.
Cite this paper
Abbas, H. , Shier, W. , Cartwright, R. and Sciumbato, G. (2014) Ustilaginoidea virens Infection of Rice in Arkansas: Toxicity of False Smut Galls, Their Extracts and the Ustiloxin Fraction. American Journal of Plant Sciences, 5, 3166-3176. doi: 10.4236/ajps.2014.521333. 
 

[1] International Rice Research Institute (2014) Rice Production Course: Importance of Rice.
www.knowledgebank.irri.org/ericeproduction/Importance_of_Rice.htm
[2] Yunich, R. (2011) Rice Industry Adds $34 Billion to Nation’s Economy. USA Rice Federation, 1-4.
www.usarice.com
[3] Lee, F.N. and Gunnell, P.S. (1992) False Smut. In: Webster, P.K. and Gunnell, P.S., Eds., Compendium of Rice Diseases, American Phytopathological Society Press, St. Paul, 28.
[4] Kou, Y. and Wang, S. (2012) Toward an Understanding of the Molecular Basis of Quantitative Disease Resistance in Rice. Journal of Biotechnology, 159, 283-290.
[5] Sanghera, G.S., Ahanger, M.A., Kashyap, S.C., Bhat, Z.A., Rather, A.G. and Parray, G.A. (2012) False Smut of Rice (Ustilaginoidea virens) under Temperate Agro-Climatic Conditions of Kashmir, India. Elixir Bio Technology, 49, 9827-9831.
[6] White, J.F., Sullivan, R., Moy, M., Patel, R. and Duncan, R. (2000) An Overview of Problems in the Classification of Plant-Parasitic Clavicipitaceae. Studies in Mycology, 45, 95-105.
[7] Ashizawa, T., Takahashi, M., Moriwaki, J. and Hirayae, K. (2011) A Refined Inoculation Method to Evaluate False Smut Resistance in Rice. Journal of General Plant Pathology, 77, 10-16.
http://dx.doi.org/10.1007/s10327-010-0279-5
[8] Ashizawa, T., Takahashi, M., Arai, M. and Arie, T. (2012) Rice False Smut Pathogen, Ustilaginoideavirens, Invades through Small Gap at the Apex of a Rice Spikelet before Heading. Journal of General Plant Pathology, 78, 255-259.
http://dx.doi.org/10.1007/s10327-012-0389-3
[9] Ladhalaskshmi, D., Laha, G.S., Singh, R., Karthikeyan, A., Mangrauthia, S.K., Sundaram, R.M., Thukkaiyannan, P. and Viraktamath, B.C. (2012) Isolation and Characterization of Ustilaginoideavirens and Survey of False Smut Disease of Rice in India. Phytoparasitica, 40, 171-176.
http://dx.doi.org/10.1007/s12600-011-0214-0
[10] Hu, M., Luo, L., Wang, S., Liu, Y. and Li, J. (2013) Infection Processes of Ustilaginoideavirens during Artificial Inoculation of Rice Panicles. European Journal of Plant Pathology, 139, 67-77.
http://dx.doi.org/10.1007/s10658-013-0364-7
[11] Fan, J., Guo, Y., Huang, F., Li, Y., Liu, F., Li, L., Xu, Y.J., Zhao, J.Q., Xiong, H., Yu, J.J. and Wang, W. (2014) Epiphytic Colonization of Ustilaginoidea virens on Biotic and Abiotic Surfaces Implies the Widespread Presence of Primary Inoculums for Rice False Smut Disease. Plant Pathology, 63, 937-945.
[12] Guo, X., Li, Y., Fan, J., Li, L., Huang, F. and Wang, W. (2012) Progress in the Study of False Smut Disease in Rice. Journal of Agricultural Science and Technology, 2, 1211-1217.
[13] Fu, R., Ding, L., Zhu, J., Li, P. and Zheng, A. (2012) Morphological Structure of Propagules and Electrophoretic Karotype Analysis of False Smut Villosiclava virens in Rice. Journal of Microbiology, 50, 263-269.
http://dx.doi.org/10.1007/s12275-012-1456-3
[14] Biswas, M.C. (2001) False Smut Disease of Rice: A Review. Environment and Ecology, 19, 67-83.
[15] Takahashi, Y. (1896) On Ustilago virens Cooke and a New Species of Tilletia Parasitic on Rice Plant. Shokubutsugaku Zasshi, 10, 16-20.
http://dx.doi.org/10.15281/jplantres1887.10.109_16
[16] Hashioka, Y. (1971) Rice Disease in the World, VIII. Diseases Due to Hypocreals, Ascomycetes (Fungal Diseases, No. 5). Riso, 20, 235-258.
[17] Tanaka, E., Ashizawa, T., Sonoda, R. and Tanaka, C. (2008) Villosiclava virens gen. nov., comb. nov. Teleomorph of Ustilaginoidea virens, the Causal Agent of Rice False Smut. Mycotaxon, 106, 491-501.
[18] Abbas, H.K., Sciumbato, G.L. and Keeling, B. (2002) First Report of False Smut of Corn (Zea mays) in the Mississippi Delta. Plant Disease, 86, 1179.
http://dx.doi.org/10.1094/PDIS.2002.86.10.1179B
[19] Pataky, J.K. (1999) False Smut. In: White, D.G., Ed., Compendium of Corn Diseases, 3rd Edition, American Phytopathological Society Press, St. Paul, 35.
[20] Cooke, M.C. (1878) Some Extra—European Fungi. Grevillea, 7, 13-15.
[21] Zhou, Y.L., Pan, Y.J., Xie, X.W., Zhu, L.H., Wang, S. and Li, Z.K. (2008) Genetic Diversity of Rice False Smut Fungus Ustilaginoidea virens and Its Pronounced Differentiation of Populations in North China. Journal of Phytopathology, 156, 559-564.
http://dx.doi.org/10.1111/j.1439-0434.2008.01387.x
[22] Li, W.L., Li, L.Y., Feng, A.Q., Zhu, X.Y. and Li, J.X. (2013) Rice False Smut Fungus, Ustilaginoidea virens, Inhibits Pollen Germination and Degrades the Integuments of Rice Ovule. American Journal of Plant Sciences, 4, 2295-2304.
http://dx.doi.org/10.4236/ajps.2013.412284
[23] Orton, W.A. (1906) Plant Diseases of 1906. Year Book US Department of Agriculture, US Government Printing Office, Washington DC, 499-508.
[24] Ahonsi, M.O., Adeoti, A.A., Erinle, I.D., Alegbejo, T.A., Singh, B.N. and Sy, A.A. (2000) Effect of Variety and Sowing Date on False Smut Incidence in Upland Rice in Edo State, Nigeria. International Rice Research Notes, 25, 14.
[25] Wang, D.W., Wang, S. and Fu, J.F. (2004) Research Advances on False Smut of Rice. Liaoning Agricultural Sciences, 1, 21-24.
[26] Koiso, Y., Li, Y., Iwasaki, S., Hanaoka, K., Kobayash, T., Sonoda, R., Fujita, Y., Yabgashi, H. and Sato, Z. (1994) Ustiloxins, Antimitotic Cyclic Peptides from False Smut Balls on Rice Panicles Caused by Ustilaginoidea virens. Journal of Antibiotics, 47, 765-773.
http://dx.doi.org/10.7164/antibiotics.47.765
[27] Koiso, Y., Natori, M., Iwasaki, S., Sato, S., Sonoda, R., Fujita, R., Yaegashi, H. and Sato, Z. (1992) Ustiloxin: A Phytotoxin and a Mycotoxin from False Smut Balls on Rice Panicles. Tetrahedron Letters, 33, 4157-4160.
http://dx.doi.org/10.1016/S0040-4039(00)74677-6
[28] Shan, T.J., Sun, W.B., Liu, H., Gao, S., Lu, S.Q., Wang, M.G., Sun, W.X., Chen, Z.Y., Wang, S. and Zhou, L.G. (2012) Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice. International Journal of Molecular Sciences, 13, 11275-11287.
http://dx.doi.org/10.3390/ijms130911275
[29] Shan, T.J., Sun, W.B., Wang, X.H., Fu, X.X., Sun, W.X. and Zhou, L.G. (2013) Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins. Molecules, 18, 8181-8199.
http://dx.doi.org/10.3390/molecules18078181
[30] Takahashi, M., Ashizawa, T., Yamanaka, N., Arai, M. and Takahashi, Y. (2012) Pathogenic Isolates of Ustilaginoidea virens Differ Greatly in Their Production of Ustiloxin A in Rice False Smut Balls. Japanese Journal of Phytopathology, 78, 305-308.
http://dx.doi.org/10.3186/jjphytopath.78.305
[31] Nakamura, K., Izumiyama, N., Ohtsubo, K., Koiso, Y., Iwasaki, S., Sonoda, R., Fujitia, Y., Yaegashi, H. and Sato, K. (1992) Lupinosis in Mice Caused by Ustiloxin and a Crude Extract of Fungal Culture of Ustilaginoidea virens (in Japanese with English Summary). Proceedings of the Japanese Association of Mycotoxicology, 35, 41-43.
[32] Li, Y., Koiso, Y., Kobayashi, H., Hashimoto, Y. and Iwasaki, S. (1995) Ustiloxins, New Antimitotic Cyclic Peptides: Interaction with Porcine Brain Tubulin. Biochemical Pharmacology, 49, 1367-1372.
http://dx.doi.org/10.1016/0006-2952(95)00072-8
[33] Abbas, H.K., Cartwright, C.D., Sciumbato, G.L., Keeling, B., Vesonder, R.F. and Shier, W.T. (2000) Mycotoxins in False Smut Balls From Rice. Phytopathology, 90, S1.
[34] Rush, M.C., Shahjahan, A.K.M., Groth, D.E. and Jones, J.P. (2000) Outbreak of False Smut of Rice in Louisiana. Plant Disease, 84, 100.
http://dx.doi.org/10.1094/PDIS.2000.84.1.100D
[35] Tanaka, T., Abbas, H.K. and Duke, S.O. (1993) Structure-Dependent Phytotoxicity of Fumonisins and Related Compounds in a Duckweed Bioassay. Phytochemistry, 33, 779-785.
http://dx.doi.org/10.1016/0031-9422(93)85274-U
[36] Abbas, H.K., Mirocha, C.J. and Shier, W.T. (1984) Mycotoxins Produced from Fungi Isolated from Foodstuffs and Soil: Comparison of Toxicity in Fibroblasts and Rat Feeding Test. Applied and Environmental Microbiology, 48, 654-661.
[37] Abbas, H.K., Shier, W.T. and Mirocha, C.J. (1984) Sensitivity of Cultured Human and Mouse Fibroblasts to Trichothecenes. , 67, 607-610.
[38] Abbas, H.K., Yoshizawa, T. and Shier, W.T. (2013) Cytotoxicity and Phytotoxicity of Trichothecene Mycotoxins Produced by Fusarium spp. Toxicon, 74, 68-75.
http://dx.doi.org/10.1016/j.toxicon.2013.07.026
[39] Abbas, H.K. and Shier, W.T. (2009) Chapter 3. Mycotoxin Contamination of Agricultural Products in the Southern United States and Approaches to Reducing It from Pre-Harvest to Final Food Products. In: Appell, M., Kendra, D. and Trucksess, M.W., Eds., Mycotoxin Prevention and Control in Agriculture. American Chemical Society Symposium Series 1031, Oxford University Press, Oxford, 37-58.
[40] Abbas, H.K., Cartwright, R.D., Windham, G.L., Xie, W., Shier, W.T. and Mirocha, C.J. (2000) The Presence of Mycotoxins and Fungi in Rice and Corn in the Southern United States. Bulletin of the Institute for Comprehensive Agricultural Sciences Kinki University, 8, 23-28.         eww141028lx
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