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A New Epidemiological Tool for Staphylococcus aureus Surveillance

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http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50441#.VD9UuFfHRK0

Epidemiological surveillance for microbes is currently based on either agar culture followed by identification, or genetic amplification. Both techniques are highly skilled-labor intensive, costly, and must be done in central laboratories. The Defined Substrate Utilization® (DSU®) format provides an epidemiological series of specific screening formulations that obviate these limitations. All reagents are present in optimized stable powder form in a test tube—add water, inoculate, and incubate. A specific color change provides a sensitive and specific detection of the target microbe. Two DSU® methods for Staphylococcus aureus (S. aureus) are presented: aureusAlert® for all S. aureus and EPI-M® for methicillin-resistant S. aureus (MRSA). Both aureusAlert® and EPI-M® had a detection level of 20 colony forming units (CFU) in 18 hours. aureusAlert® and conventional methods agreed 93.6% and EPI-M® and conventional methods agreed 94.1%. DSU® and conventional methods showed the same specificity.
Cite this paper
Edberg, S. and Miller, J. (2014) A New Epidemiological Tool for Staphylococcus aureus Surveillance. Advances in Microbiology, 4, 919-924. doi: 10.4236/aim.2014.413102. 
 

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