跳至主要内容

Utilizing Soybean Milk to Culture Soybean Pathogens

There are a number of publications that report on how to culture plant pathogens on various types of growth media. The ingredients of culture media, natural or synthetic, along with other factors like light, pH, and temperature, will affect the growth of microbes including vegetative growth and production of reproductive (asexual or sexual) structures or other survival structures. In recent years, media containing soybean products have been used to culture microbes including plant pathogens, however, culture media made from soymilk have not been tested for culturing soybean pathogens.

In this study, a comparison was made between soybean milk medium, also referred to as soymilk, and media traditionally used for culturing soybean pathogens to determine if soymilk medium was an effective medium for growth of Colletotrichum truncatum, Fusarium virguliforme, Macrophomina phaseolina, Passalora sojina, Phomopsis longicolla, Phytophthora sojae, Pythium irregulare, Rhizoctonia solani, and Sclerotinia sclerotiorum. Each experiment was repeated and data from repeated experiments were combined if there was no significant interaction between trials and treatments and if error variances were homogeneous in Bartlett’s test for homogeneity.

Based on radial mycelial colony growth rates, C. sojina grew significantly (P < 0.05) faster on soymilk dextrose agar (SDA) than on V-8 agar, and C. truncatum and F. virguliforme grew significantly (P < 0.05) faster on SDA than potato dextrose agar (PDA). Significantly (P < 0.001) greater masses of sclerotia were produced by S. sclerotiorum grown on SDA as compared to PDA.

In conclusion, a medium made from soybean milk was an effective medium for growth of all the facultative soybean pathogens tested. Soymilk used with agar or used alone as a broth may be a viable substitute to replace more expensive processed media.

Future studies need to explore more potential uses of soymilk in culturing microbes. This could include studies on soymilk concentration in medium and how that affects fungal growth and reproduction, and to determine if soymilk could be used to culture a wide range of microbes including those used as biological control agents, where cheap growth media may be of interest.

Article by Yiwen Xiang, et al, from University of Illinois, Urbana, USA.

Full access: http://mrw.so/3je2bT
Image by Lauren, from Flickr-cc.

评论

发表评论

此博客中的热门博文

A Comparison of Methods Used to Determine the Oleic/Linoleic Acid Ratio in Cultivated Peanut (Arachis hypogaea L.)

Cultivated peanut ( Arachis hypogaea L.) is an important oil and food crop. It is also a cheap source of protein, a good source of essential vitamins and minerals, and a component of many food products. The fatty acid composition of peanuts has become increasingly important with the realization that oleic acid content significantly affects the development of rancidity. And oil content of peanuts significantly affects flavor and shelf-life. Early generation screening of breeding lines for high oleic acid content greatly increases the efficiency of developing new peanut varieties. The objective of this study was to compare the accuracy of methods used to classify individual peanut seed as high oleic or not high oleic. Three hundred and seventy-four (374) seeds, spanning twenty-three (23) genotypes varying in oil composition (i.e. high oleic (H) or normal/not high oleic (NH) inclusive of all four peanut market-types (runner, Spanish, Valencia and Virginia), were individually tested ...
发表评论
阅读全文

Location Optimization of a Coal Power Plant to Balance Costs against Plant’s Emission Exposure

Fuel and its delivery cost comprise the biggest expense in coal power plant operations. Delivery of electricity from generation to consumers requires investment in power lines and transmission grids. Placing a coal power plant or multiple power plants near dense population centers can lower transmission costs. If a coalmine is nearby, transportation costs can also be reduced. However, emissions from coal plants play a key role in worsening health crises in many countries. And coal upon combustion produces CO 2 , SO 2 , NO x , CO, Metallic and Particle Matter (PM10 & PM2.5). The presence of these chemical compounds in the atmosphere in close vicinity to humans, livestock, and agriculture carries detrimental health consequences. The goal of the research was to develop a methodology to minimize the public’s exposure to harmful emissions from coal power plants while maintaining minimal operational costs related to electric distribution losses and coal logistics. The objective was...
发表评论
阅读全文

Evaluation of the Safety and Efficacy of Continuous Use of a Home-Use High-Frequency Facial Treatment Appliance

At present, many home-use beauty devices are available in the market. In particular, many products developed for facial treatment use light, e.g., a flash lamp or a light-emitting diode (LED). In this study, the safety of 4 weeks’ continuous use of NEWA TM , a high-frequency facial treatment appliance, every alternate day at home was verified, and its efficacy was evaluated in Japanese individuals with healthy skin aged 30 years or older who complained of sagging of the facial skin.  Transepidermal water loss (TEWL), melanin levels, erythema levels, sebum secretion levels, skin color changes and wrinkle improvement in the facial skin were measured before the appliance began to be used (study baseline), at 2 and 4 weeks after it had begun to be used, and at 2 weeks after completion of the 4-week treatment period (6 weeks from the study baseline). In addition, data obtained by subjective evaluation by the subjects themselves on a visual analog scale (VAS) were also analyzed. Fur...
发表评论
阅读全文