Biopharmaceutical Assessment of Active Components of Deadaleopsis confragosa and Ganoderma lucidum

The spread of multidrug-resistant strains of bacteria makes it necessary to discover new classes of antibacterial and compounds that inhibit these resistant mechanisms. Hence, this study investigated the antimicrobial activities of Ganoderma lucidum and Deadaleopsis confragosa extracts against some bacterial isolates of medical importance.

The samples used for this research work were obtained on the 16th April, 2010 from a cocoa farm very close to Joseph Ayo Babalola University, Ikeji-Arakeji, Osun State, Nigeria. They were washed with distilled water and sundried for four days. Then they were oven dried for 24 hr at 45˚C. The dried samples were pulverized using industrial blender (exceler). By using agar well diffusion assay, aqueous, ethanolic and petroleum ether extracts were obtained from Ganoderma lucidum and Daedaleopsis confragosa and assayed for antimicrobial on five bacterial species, viz: Pseudomonas aeruginosa, Escherichia coli, Staphyloccoccus aureus, Proteus mirabilis and Klebsiella pneumoniae. In vitro bioassay revealed that the aqueous extract of G. lucidum inhibited P. aeruginosa S. aureus, E. coli and K. Pneumoniae with inhibition zones of 11.0 ± 0.02 mm, 10.0 ± 0.02 mm, 13.0 ± 0.03 mm and 14.0 ± 0.0 mm respectively. The ethanolic extract of G. lucidum also inhibited P. aeruginosa, S. aureus and E. coli with inhibition zones 12.0 ± 0.01 mm, 11.0 ± 0.02 mm and 16.0 ± 0.01 mm. Petroleum ether extract of G. lucidum inhibited P. aeruginosa, S. aureus and E. coli with inhibition zones of 12.0 ± 0.01 mm, 11.0 ± 0.03 mm and 12.0 ± 0.02 mm. For Daedaleopsis confragosa, the aqeous extract inhibited P. aeruginosa and E.coli with inhibition zones of 12.0 ± 0.01 and 12.0 ± 0.02 mm respectively while the petroleum ether extract inhibited S. aureus and E. coli with inhibition zones of 19.0 ± 0.02 mm and 13.0 ± 0.01 mm respectively. 

In conclusion, all these inhibitions on clinical isolates are therefore attributed to the presence of some bioactive compounds as shown by the phytochemical screening of the G. lucidum and D. confragosa which include tannins, phenolics, flavonoids and saponin.

Article by Soji Fakoya, et al, from Nigeria.

Full access: http://mrw.so/1mnM2T

Image by Tatiana Bulyonkova, from Flickr-cc.