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Production of Fermentable Sugars from Organosolv Pretreated Cassava Peels

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ABSTRACT
Cassava peels are rich in lignocellulolytic materials which are not readily amenable to enzymatic hydrolysis; hence, there is a need for a suitable pretreatment method that will support enzymatic hydrolysis. This study was designed to investigate lignocellulolytic organisms that would effectively support the bioconversion of organosolv pretreated cassava peels to fermentable sugars. Decaying cassava peels were collected into sterile bottles and microorganisms isolated, characterized and screened for lignocellulolytic enzymes production. Optimum temperature, pH and nutrient sources for enzyme production were determined. Organosolv pretreatment was carried out using methanol with varied concentration of catalyst (0.01 - 3 M), reaction time (15 - 60 min) and substrate size. Crude enzymes (cellulase and xylanase) from the isolates were added to the pretreated peels and bioconversion was monitored by measuring the concentration of reducing sugar and calculating the percentage peel hydrolysis. The fermentable sugars produced were quantified using gas chromatography. Pseudomonas fluorescens and Aspergillus terreus were isolated. P. fluorescens produces 2.8 u/mL of crude enzymes optimally at 50°C and pH 8 while A. terreus produces 3.4 u/mL optimally at 40°C, pH 6. Both isolates utilizes CarboxyMethylCellulose (CMC) and yeast extract as their best carbon and nitrogen sources. Highest percentage of peel hydrolysis was 67% for P. fluorescens at 0.01 M and 0.05 M for A. terreus (94%). Highest concentration of fermentable sugar was produced by A. terreus crude enzyme (331.79 mg/L glucose, 45.3 mg/L rhamnose and 46.52 mg/L xylose). P. fluorescens and A. terreus effectively supported the bioconversion of organosolv pretreated cassava peels to fermentable sugars.
 
Cite this paper
Atinuke, O. and Ayodele, O. (2015) Production of Fermentable Sugars from Organosolv Pretreated Cassava Peels. Advances in Microbiology, 5, 117-122. doi: 10.4236/aim.2015.52012.
 
References
[1]Adegbola, A.A. and Asaolu, V.O. (1986) Preparation of Cassava Peels for Use in Small Ruminant Production in Western Nigeria. In: Preston, T.R. and Nuwanyakpa, M.Y., Eds., Towards Optimal Feeding of Agricultural By-Products to Livestock in Africa, Proceedings of a Workshop Held at the University of Alexandria, Egypt, Addis Ababa, 109-115.
 
[2]Odunfa, S.A. and Shasore, S.B. (1987) Saccharification of Cassava Peels Waste for Microbial Protein. Acta Biotechnologica, 7, 23-29.
 
[3]Kongkiattikajorn, J. and Sornvoraweat, B. (2011) Comparative Study of Bioethanol Production from Cassava Peels by Monoculture and Co-Culture of Yeast. Kasetsart Journal (Natural Science), 45, 268-274.
 
[4]Wyman, C.E. (1999) Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges. Annual Review of Energy and the Environment, 24, 189-226.
http://dx.doi.org/10.1146/annurev.energy.24.1.189
 
[5]Mosier, N.S., Wyman, C., Dale, B., Elander, R., Lee, Y.Y., Holtzapple, M. and Ladisch, M.R. (2005) Features of Promising Technologies for Pretreatment of Lignocellulosic Biomass. Bioresource Technology, 96, 673-686.
http://dx.doi.org/10.1016/j.biortech.2004.06.025
 
[6]Botello, J.I., Gilarranz, M.A., Rodriguez, F. and Oliet, M. (1999) Preliminary Study on Products Distribution in Alcohol Pulping of Eucalyptus globulus. Journal of Chemical Technology and Biotechnology, 74, 141-148.
http://dx.doi.org/10.1002/(SICI)1097-4660(199902)74:2<141::AID-JCTB1>3.0.CO;2-0
 
[7]Harmsen, P.F.H., Huijgen, W.J.J., Bermudez, L.L.M. and Bakker, R.R.C. (2010) Literature Review of Physical and Chemical Pretreatment Process for Lignocellulosic Biomass. Food and Biobased Research Energy Research Centre of Netherland, 49 p.
 
[8]Sarkanen, K.V. (1980) Acid-Catalyzed Delignification of Lignocellulosics in Organic Solvents. Progress in Biomass Conversion, 2, 127-144.
http://dx.doi.org/10.1016/B978-0-12-535902-3.50010-5
 
[9]Ghose, T.K., Pannir Selvam, P.V. and Ghosh, P. (1983) Catalytic Solvent Delignification of Agricultural Residues: Organic Catalysts. Journal of Biotechnology and Bioengineering, 25, 2577-2590.
http://dx.doi.org/10.1002/bit.260251108
 
[10]Zhao, X.B., Cheng, K.K. and Liu, D.H. (2009) Organosolv Pretreatment of Lignocellulosic Biomass for Enzymatic Hydrolysis. Applied Microbiology and Biotechnology, 82, 815-827.
http://dx.doi.org/10.1007/s00253-009-1883-1
 
[11]Onilude, A.A. (1999) Production, Characterization and Utilization of Some Dietary Fiber Degrading Layers Fed Biodegraded Cassava Root Sieviate. Ph.D. Thesis, Department of Botany and Microbiology, University of Ibadan, Ibadan.
 
[12]Miller, G.L. (1959) Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Journal of Analytical Chemistry, 31, 426-428.
http://dx.doi.org/10.1021/ac60147a030
 
[13]Laurence, D.M. and Bronwen, G.S. (2001) Current Protocols in Food Analytical Chemistry. John Wiley & Sons, Inc., Hoboken.
 
[14]Hoorman, J. (2011) Nutrient Recycling with Manure and Cover Crops. Journal of Cover Crops and Soil Organic Matter, 4, 22.
 
[15]Gupta, R. and Mukergi, K.G. (2001) Industrial Microbiology; Microbial Technology. 78.
 
[16]Sun, Y. and Cheng, J.Y. (2002) Hydrolysis of Lignocellulosic Materials for Ethanol Production: A Review. Bioresource Technology, 83, 1-11.
http://dx.doi.org/10.1016/S0960-8524(01)00212-7
 
[17]Taherzadeh, M.J. and Keikhosro, K. (2008) Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review. International Journal of Molecular Sciences, 9, 1621-1651.
http://dx.doi.org/10.3390/ijms9091621
 
[18]Patiwat, C. and Chalerm, R. (2010) A Study on Cellulosic Ethanol Production from Durian Peels Using Saccharomyces cerevisiae. 36th Congress on Science and Technology of Thailand, Bangkok, 26-28 October 2010.
 
[19]Lin, Y. and Tanaka, S. (2006) Ethanol Fermentation from Biomass Resources: Current State and Prospects. Journal Applied Microbiology Biotechnology, 69, 627-642.
http://dx.doi.org/10.1007/s00253-005-0229-x                                          eww150213lx
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