Microbial Observation in Bioaccumulation of Heavy Metals from the Ash Dyke of Thermal Power Plants of Chhattisgarh, India
Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54120#.VO2HrizQrzE
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54120#.VO2HrizQrzE
Affiliation(s)
1Department of Life Science, Dr. C. V. Raman University, Bilaspur, India.
2Department of Biotechnology, Barkatullah University, Bhopal, India.
3Department of Botany, Jawaharlal Nehru Agriculture University, Jabalpur, India.
2Department of Biotechnology, Barkatullah University, Bhopal, India.
3Department of Botany, Jawaharlal Nehru Agriculture University, Jabalpur, India.
ABSTRACT
Several
mechanisms are developed by the microorganisms to tolerate few high
concentrations of heavy metals. One of these mechanisms dependent upon
anabolic and catabolic energy of microorganisms is the bioaccumulation
of heavy metals. In present work, approximately four varieties of
bacteria have been isolated from the ash dyke sample of four thermal
power plants of Chhattisgarh, i.e., Bharat Aluminium Company
(BALCO), Chhattisgarh State Electricity Board (CSEB), Korba, Thermal
Power Cooperation (NTPC), Bilaspur and KSK Akaltara, Chattisgarh. Out of
one hundred fifty isolates, three were capable to grow in varying
concentration of heavy metals. The strains were tested for their
tolerance against six different types of heavy metals dominant in the
ash samples viz. Pb, Hg, Ni, Co, Cu, Mn. Their maximum resistance
existed up to 0.6mM/ml of the above mentioned different metals under lab
standard conditions. Three isolates are found suitable for the multiple
metal resistance ability viz SM2, SM3, and SM12. These are categorized as Bacillus cereus (SM2, SM3), and Bacillus subtilis (SM12) after performing 16S rDNA sequencing.
Cite this paper
References
Banerjee,
S. , Gothalwal, R. , Sahu, P. and Sao, S. (2015) Microbial Observation
in Bioaccumulation of Heavy Metals from the Ash Dyke of Thermal Power
Plants of Chhattisgarh, India. Advances in Bioscience and Biotechnology, 6, 131-138. doi: 10.4236/abb.2015.62013.
| [1] | Rajaganapathy,
V., Xavier, F., Sreekumar, D. and Mandal, P.K. (2011) Heavy Metal
Contamination from Soil, Water and Fodder and Their Presence in
Livestocks and Products: A Review. Journal of Environmental Science and
Technology, 4, 234-249. http://dx.doi.org/10.3923/jest.2011.234.249 |
| [2] | Lee,
J.T., Son, J.Y. and Cho, Y.S. (2007) The Adverse Effects of Fine
Particle Air Pollution on Respiratory Function in the Elderly. Science
Total Environment, 385, 28-36. http://dx.doi.org/10.1016/j.scitotenv.2007.07.005 |
| [3] | Pandey,
V.C., Singh, J.S., Kumar, A. and Tewari, D.D. (2010) Accumulation of
Heavy Metals by Chickpea Grown in Fly Ash Treated Soil: Effects on
Antioxidants. Clean Soil Air Water, 38, 1116-1123. http://dx.doi.org/10.1002/clen.201000178 |
| [4] | Kumar, A., Bisht, B.S., Joshi, V.D. and Dhewa, T. (2011) Review on Bioremediation of Polluted Environment: A Management Tool. International Journal of Environmental Sciences, 1, 1079-1093. |
| [5] | Ogbo,
E.M. and Okhuoya, J.A. (2011) Bio-Absorption of Some Heavy Metals by
Pleurotus tuber-regium Fr. Singer (an Edible Mushroom) from Crude Oil
Polluted Soils Amended with Fertilizers and Cellulosic Wastes.
International Journal of Soil science, 6, 34-48. http://dx.doi.org/10.3923/ijss.2011.34.48 |
| [6] | Subroto,
M.A., Santoso, P. and Siswi, I.N. (2007) Accumulation of Zinc by Hairy
Root Cultures of Solanum nigrum. Biotechnology, 6, 344-348. http://dx.doi.org/10.3923/biotech.2007.344.348 |
| [7] | Bhattacharya,
A.K., Mandal, S.N. and Das, S.K. (2008) Heavy Metals Accumulation in
Water Sediment and Tissues of Different Edible Fishes in Upper Stretch
of Gangetic West Bengal. Trends in Applied Science Research, 3, 61-68. http://dx.doi.org/10.3923/tasr.2008.61.68 |
| [8] | Hashem,
A.R. and Abed, K.F. (2002) Arsenic, Lead and Microorganisms in Hair and
Nails of Some Women from Saudi Arabia. Journal of Medical Science, 2,
82-84. http://dx.doi.org/10.3923/jms.2002.82.84 |
| [9] | Rani,
A. and Goel, R. (2009) Strategies for Crop Improvement in Contaminated
Soils Using Metal Tolerant Bioinoculants. In: Khan, M.S., Zaidi, A. and
Mussarat, J., Eds., Microbial Strategies for Crop Improvement, Springer,
Berlin, 105-132. http://dx.doi.org/10.1007/978-3-642-01979-1_5 |
| [10] | Matyar,
F., Akkan, T., Ucak, Y. and Eraslan, B. (2010) Aeromonas and
Pseudomonas: Antibiotic and Heavy Metal Resistance Species from
Iskenderun Bay, Turkey (Northeast Mediterranean Sea). Environmental
Monitoring Assessment, 167, 309-320. http://dx.doi.org/10.1007/s10661-009-1051-1 |
| [11] | Ahmed,
M. and Khan, M.S. (2011) Pesticide Interactions with Soil Microflora:
Importance in Bioremediation. In: Ahmad, I., Ahmad, F. and Pichtel, J.,
Eds., Microbes and Microbial Technology: Agricultural and Environmental
Applications, Springer, New York, 393-413. http://dx.doi.org/10.1007/978-1-4419-7931-5_15 |
| [12] | Nies, D.H. (1999) Microbial Heavy Metal Resistance. Applied Microbiology and Biotechnology, 51, 730-750. http://dx.doi.org/10.1007/s002530051457 |
| [13] | Gadd, G.M. (1992) Metals and Microorganisms: A Problem of Definition. FEMS Microbiology Letters, 100, 197-203. http://dx.doi.org/10.1111/j.1574-6968.1992.tb14040.x |
| [14] | Issazadeh, K., Pahlaviani, M.R.M.K. and Massiha, A. (2011) Bioremediation of Toxic Heavy Metals Pollutants by Bacillus spp. Isolated from Guilan Bay Sediments, North of Iran. International Conference on Biotechnology and Environment Management ICBEE, 18, 67-71. |
| [15] | Costa,
A.A.C. and Pereira, D.F. (2001). Bioaccumulation of Copper, Zinc,
Cadmium and Lead by Bacillus sp., Bacillus cereus, Bacillus sphaerecus
and Bacillus subtilis. Brazilian Journal of Microbiology, 32, 1-5. http://dx.doi.org/10.1590/S1517-83822001000100001 |
| [16] | Unaldi Coral, M.N., Korkmaz, H., Arikan, B. and Coral, G. (2005) Plasmid Mediated Heavy Metal Resistance in Enterobacter spp. Isolated from Sofulu Landfill, in Adana, Turkey. Annals of Microbiology, 55, 175-179. |
| [17] | Zolgharnein,
H., Karami, K., Assadi, M.M. and Sohrab, A.D. (2010) Investigation of
Heavy Metals Biosorption on Pseudomonas aeruginosa Strain MCCB 102
Isolated from the Persian Gulf. Asian Journal of Biotechnology, 2,
99-109. http://dx.doi.org/10.3923/ajbkr.2010.99.109 |
| [18] | Bahig, A.E., Aly, E.A., Khaled, A.A. and Amel, K.A. (2008) Isolation, Characterization and Application of Bacterial Population from Agricultural Soil at Sohag Province, Egypt. Malaysian Journal of Microbiology, 4, 42-50. |
| [19] | Jiang,
C., Sheng, X., Qian, M. and Wang, Q. (2008) Isolation and
Characterization of a Heavy Metal-Resistant Burkholderia sp. from Heavy
Metal-Contaminated Paddy Field Soil and Its Potential in Promoting Plant
Growth and Heavy Metal Accumulation in Metal-Polluted Soil.
Chemosphere, 72, 157-164. http://dx.doi.org/10.1016/j.chemosphere.2008.02.006 |
| [20] | Kamala-Kannan,
S. and Lee, K.J. (2008) Metal Tolerance and Antibiotic Resistance of
Bacillus Species Isolated from Sunchon Bay Sediments, South Korea.
Biotechnology, 7, 149-152. http://dx.doi.org/10.3923/biotech.2008.149.152 |
| [21] | Ganje, T.J. and Page, A.L. (1974) Rapid Acid Dissolution of Plant Tissue for Cadmium Determination by Atomic Absorption Spectrophotometer. Atomic Absorption Newsletter, 13, 131-134. |
| [22] | Bruno,
W.J., Socci, N.A. and Halpern, A.L. (2000) Weighted Neighbor Joining: A
Likelihood-Based Approach to Distance-Based Phylogeny Reconstruction.
Molecule Biology Evolution, 17, 189-197. http://dx.doi.org/10.1093/oxfordjournals.molbev.a026231 |
| [23] | Wiley, E.O., Brooks, D.R., Siegel-Causey, D. and Funk, V.A. (1991) The Compleat Cladist: A Primer of Phylogenetic Procedures. http://taxonomy.zoology.gla.ac.uk/teaching/compleatCladist.pdf |
| [24] | Ahmed,
M. and Malik, A. (2011) Bioaccumulation of Heavy Metals by Zinc
Resistant Bacteria Isolated from Agricultural Soils Irrigated with
Wastewater. Bacteriology Journal, 2, 12-21. http://dx.doi.org/10.3923/bj.2012.12.21 |
| [25] | Vullo,
D.L., Ceretti, H.M., Daniel, M.A., Ramirez, S.A.M. and Zalts, A. (2008)
Cadmium, Zinc and Copper Biosorption Mediated by Pseudomonas veronii
2E. Bioresource Technology, 99, 5574-5581. http://dx.doi.org/10.1016/j.biortech.2007.10.060 |
| [26] | Pandey, S., Saha, P., Biswas, S. and Maiti, T.K. (2011) Characterization of Two Metal Resistant Bacillus Strains Isolated from Slag Disposal Site at Burnpur, India. Journal of Environmental Biology, 32, 773-779. |
| [27] | Singh,
S.K., Tripathi, V.R., Jain, R.K., Vikram, S. and Garg, S.K. (2010) An
Antibiotic Heavy Metal Resistant and Halotolerant Bacillus cereus SIU1
and Its Thermoalkaline Protease. Microbial Cell Factories, 9, 1-7. http://dx.doi.org/10.1186/1475-2859-9-59 |
| [28] | Habi,
S. and Daba, H. (2009) Plasmid Incidence, Antibiotic and Metal
Resistance among Enterobacteriaceae Isolated from Algerian Streams.
Pakistan Journal of Biological Science, 12, 1474-1482. http://dx.doi.org/10.3923/pjbs.2009.1474.1482 |
| [29] | Zolgharnein, H., Mohd Azmi, M.L., Saad, M.Z., Mutalib, A.R. and Mohamed, C.A.R. (2007) Detection of Plasmids in Heavy Metal Resistance Bacteria Isolated from the Persian Gulf and Enclosed Industrial Areas. Iranian Journal of Biotechnology, 5, 232-239. |
| [30] | Murthy, S., Bali, G. and Sarangi, S.K. (2014) Effect of Lead on Growth, Protein and Biosorption Capacity of Bacillus cereus Isolated from Industrial Effluent. Journal of Environmental Biology, 35, 407-411. eww150225lx |
评论
发表评论