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Factors Affecting the Induction of Lignin Peroxidase in Manganese-Deficient Cultures of the White Rot Fungus Phanerochaete chrysosporium

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ABSTRACT
The lignin peroxidase (LIP) production and regulation, in manganese ions (Mn2+) deficient cultures of the white rot fungus Phanerochaete chrysosporium, is still not clearly understood. Mn2+ deficiency is correlated to low levels of manganese containing superoxide dismutase (MnSOD). In this work, we show that despite the low activity level of MnSOD in Mn2+-deficient cultures, the presence of H2O2 is essential for the expression of the lip-H2 gene, which encodes for the major LIP isoenzyme produced (LIP-H2). Thus, the H2O2 present in Mn2+-deficient cultures is probably produced by other mechanisms rather than dismutation of superoxide ions by MnSOD. Glyoxal oxidase gene (glox) expression was significantly higher than MnSOD (MnSOD1) and cellobiose dehydrogenase (cdh1) expression in Mn2+-deficient cultures, indicating its clear involvement in H2O2 production in those cultures. Glyoxal oxidase may compensate the absence of MnSOD activity in Mn2+-deficient cultures. The high levels of reactive oxygen species (ROS) needed for the enhancement of LIP expression in Mn2+-deficient cultures were not directly correlated to the protein kinase C (PKC) activity involved in signal transduction pathway. High level of oxidative stress was observed in MnSOD silenced mutants, grown in the presence of Mn2+, indicating that oxidative stress in Mn2+-deficient cultures was caused by low levels of MnSOD rather than the deficiency in Mn2+. The results of this work can further contribute to the understanding of LIP regulation in Mn2+-deficient cultures.
 
Cite this paper
Matityahu, A. , Sitruk, A. , Hadar, Y. and Belinky, P. (2015) Factors Affecting the Induction of Lignin Peroxidase in Manganese-Deficient Cultures of the White Rot Fungus Phanerochaete chrysosporium. Advances in Microbiology, 5, 83-92. doi: 10.4236/aim.2015.52009.
 
References
[1]de Jong, E. (1993) Physiological Roles and Metabolism of Fungal Aryl Alcohols. Wageningen Agricultural University, Wageningen.
 
[2]Gold, M.H. and Alic, M. (1993) Molecular Biology of the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Microbiological Reviews, 57, 605-622.
 
[3]Cullen, D. (2002) Molecular Genetics of Lignin-Degrading Fungi and Their Applications in Organopollutant Degradation. In: The Mycota: A Comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research, Vol. 11, Agricultural Applications, Kempken, Springer, Berlin, 71-90.
 
[4]Kirk, T.K. and Farrell, R.L. (1987) Enzymatic “Combustion”: The Microbial Degradation of Lignin. Annual Review of Microbiology, 41, 465-505.
http://dx.doi.org/10.1146/annurev.mi.41.100187.002341
 
[5]Tien, M. and Kirk, T.K. (1984) Lignin-Degrading Enzyme from Phanerochaete chrysosporium: Purification, Characterization, and Catalytic Properties of a Unique H2O2-Requiring Oxygenase. Proceedings of the National Academy of Sciences of the United States of America, 81, 2280-2284.
http://dx.doi.org/10.1073/pnas.81.8.2280
 
[6]Belinky, P.A., et al. (2003) Reactive Oxygen Species and Induction of Lignin Peroxidase in Phanerochaete chrysosporium. Applied and Environmental Microbiology, 69, 6500-6506.
http://dx.doi.org/10.1128/AEM.69.11.6500-6506.2003
 
[7]Belinky, P.A., Flikshtein, N. and Dosoretz, C.G. (2006) Induction of Lignin Peroxidase via Reactive Oxygen Species in Manganese-Deficient Cultures of Phanerochaete chrysosporium. Enzyme and Microbial Technology, 39, 222-228.
http://dx.doi.org/10.1016/j.enzmictec.2005.10.023
 
[8]Rothschild, N., et al. (1999) Manganese Deficiency Can Replace High Oxygen Levels Needed for Lignin Peroxidase Formation by Phanerochaete chrysosporium. Applied and Environmental Microbiology, 65, 483-488.
 
[9]Boominatan, K. and Reddy, C.A. (1992) cAMP-Mediated Differential Regulation of Lignin Peroxidase and Manganese-Dependent Peroxidase Production in the White-Rot Basidiomycete Phanerochaete chrysosporium. Proceedings of the National Academy of Sciences of the United States of America, 89, 5586-5590.
http://dx.doi.org/10.1073/pnas.89.12.5586
 
[10]Bar-Lev, S.S. and Kirk, T.K. (1981) Effects of Molecular Oxygen on Lignin Degradation by Phanerochaete chrysosporium. Biochemical and Biophysical Research Communications, 99, 373-378.
http://dx.doi.org/10.1016/0006-291X(81)91755-1
 
[11]Dosoretz, C.G., Chen, A.H.C. and Grethlein, H.E. (1990) Effect of Oxygenation Conditions on Submerged Cultures of Phanerochaete chrysosporium. Applied Microbiology and Biotechnology, 34, 131-137.
http://dx.doi.org/10.1007/BF00170937
 
[12]Dosoretz, C.G., Rothschild, N. and Hadar, Y. (1993) Overproduction of Lignin Peroxidase by Phanerochaete chrysosporium (BKM-F-1767) under Nonlimiting Nutrient Conditions. Applied and Environmental Microbiology, 59, 1919-1926.
 
[13]Dosoretz, C.G. and Grethlein, H.E. (1991) Physiological Aspects of the Regulation of Extracellular Enzymes of Phanerochaete chrysosporium. Applied Biochemistry and Biotechnology, 28-29, 253-265.
http://dx.doi.org/10.1007/BF02922605
 
[14]Forney, L.J., Reddy, C.A., Tien, M. and Aust, S.D. (1982) The Involvement of Hydroxyl Radical Derived from Hydrogen Peroxide in Lignin Degradation by the White Rot Fungus Phanerochaete chrysosporium. Journal of Biological Chemistry, 257, 11455-11462.
 
[15]Tien, M. and Tu, C.P. (1987) Cloning and Sequencing of a cDNA for a Ligninase from Phanerochaete chrysosporium. Nature, 326, 520-523.
http://dx.doi.org/10.1038/326520a0
 
[16]Matityahu, A., Hadar, Y. and Belinky, P.A. (2010) Involvement of Protein Kinase C in Lignin Peroxidase Expression in Oxygenated Cultures of the White Rot Fungus Phanerochaete chrysosporium. Enzyme and Microbial Technology, 47, 59-63.
http://dx.doi.org/10.1016/j.enzmictec.2010.05.002
 
[17]Cullen, D. and Kersten, P.J. (2004) Enzymology and Molecular Biology of Lignin Degradation. In: The Mycota III: Biochemistry and Molecular Biology, Springer-Verlag, Berlin, 249-273.
 
[18]Matityahu, A., Hadar, Y., Dosoretz, C.G. and Belinky, P.A. (2008) Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium. Applied and Environmental Microbiology, 74, 5359-5365.
http://dx.doi.org/10.1128/AEM.02433-07
 
[19]Tien, M. and Kirk, T.K. (1988) Lignin Peroxidase of Phanerochaete chrysosporium. Methods in Enzymology, 161, 238-249.
http://dx.doi.org/10.1016/0076-6879(88)61025-1
 
[20]Rothschild, N., Hadar, Y. and Dosoretz, C.G. (1995) Ligninolytic System Formation by Phanerochaete chrysosporium in Air. Applied and Environmental Microbiology, 61, 1833-1838.
 
[21]Lee, J.S., Hah, Y.C. and Roe, J.H. (1993) The Induction of Oxidative Enzymes in Streptomyces coelicolor upon Hydrogen Peroxide Treatment. Journal of General Microbiology, 139, 1013-1018.
http://dx.doi.org/10.1099/00221287-139-5-1013
 
[22]Schmitz, H.P. and Heinisch, J.J. (2003) Evolution, Biochemistry and Genetics of Protein Kinase C in Fungi. Current Genetics, 43, 245-254.
http://dx.doi.org/10.1007/s00294-003-0403-6
 
[23]Maher, P. (2001) How Protein Kinase C Activation Protects Nerve Cells from Oxidative Stress-Induced Cell Death. Journal of Neuroscience, 21, 2929-2938.
 
[24]Georgiou, C.D., Patsoukis, N., Papapostolou, I. and Zervoudakis, G. (2006) Sclerotial Metamorphosis in Filamentous Fungi Is Induced by Oxidative Stress. Integrative and Comparative Biology, 46, 691-712.
http://dx.doi.org/10.1093/icb/icj034                                               eww150211lx
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