跳至主要内容

Molecular Phylogeny of Arctic Microbes Using Metagenomic Approach

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52832#.VKn-hsnQrzE

Metagenomic approach to the characterization of uncultured and mixed bacterial consortium has been used in this study for a few arctic micro-organisms. Arctic soil, collected from one spot at Ny Alesund in Svalbard was used as the source of such organisms and enriched in culture medium. Only psychrotrophic microbes were chosen which could grow from 5°C to 20°C. Total genomic DNA isolated from the consortium was used as template for amplifying the 16S rRNA genes using the conserved forward and reverse primers. The amplified mixture of 1.4 kb DNA was cloned in pGEMT Easy vector and individual 16S rDNA sequences were determined by automated sequencing. A phylogenetic tree was constructed using MEGA 5 software which enlists 19 isolates in the consortium. Most of these arctic bacteria were Bacillus species as these were isolated from a single spot.
Cite this paper
Ray, D. , Mohandass, S. and Roy, P. (2014) Molecular Phylogeny of Arctic Microbes Using Metagenomic Approach. Advances in Microbiology, 4, 1278-1284. doi: 10.4236/aim.2014.416137. 
 

[1] Whitman, W.B., Coleman, D.C. and Wiebe, W.J. (1998) Prokaryotes: The Unseen Majority. Proceedings of the National Academy of Sciences of the United States of America, 95, 6578-6583.
http://dx.doi.org/10.1073/pnas.95.12.6578
[2] Turnbaugh, P.J. and Gordon, J.I. (2008) An Invitation to the Marriage of Metagenomics and Metabolomics. Cell, 134, 708-713.
[3] Sleator, R.D., Shortall, C. and Hill, C. (2008) Metagenomics. Letters in Applied Microbiology, 47, 361-366. http://dx.doi.org/10.1111/j.1472-765X.2008.02444.x
[4] Venter, J.C., et al. (2004) Environmental Genome Shotgun Sequencing of the Sargasso Sea. Science, 304, 66-74. http://dx.doi.org/10.1126/science.1093857
[5] Daniel, R. (2005) The Metagenomics of Soil. Nature Reviews Microbiology, 3, 470-478.
http://dx.doi.org/10.1038/nrmicro1160
[6] Ferrer, M., Beloqui, A., Timmis, K.N. and Golyshin, P.N. (2009) Metagenomics for Mining New Genetic Resources of Microbial Communities. Journal of Molecular Microbiology and Biotechnology, 16, 109-123. http://dx.doi.org/10.1159/000142898
[7] Handelsman, J. (2004) Metagenomics: Application of Genomics to Uncultured Microorganisms. Microbiology and Molecular Biology Reviews, 68, 669-685.
http://dx.doi.org/10.1128/MMBR.68.4.669-685.2004
[8] Pace, N.R., Stahl, D.A., Lane, D.J. and Olsen, G.J. (1985) Analyzing Natural Microbial Populations by rRNA Sequences. ASM News, 51, 4-12.
[9] Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
[10] Mezei, L.M. and Storts, D.R. (1994) Purification of PCR Products. In: Griffin, H.G. and Griffin, A.M., Eds., PCR Technology: Current Innovations, CRC Press, Boca Raton, 21-27.
[11] Birnboim, H.C. and Doly, J. (1979) A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA. Nucleic Acids Research, 7, 1513-1523. http://dx.doi.org/10.1093/nar/7.6.1513
[12] Lane, D.J., Pace, B., Olsen, G.J., Stahl, D.A., Sogin, M.L. and Pace, N.R. (1985) Rapid Determination of 16S Ribosomal RNA Sequences for Phylogenetic Analysis. Proceedings of the National Academy of Sciences of the United States of America, 82, 6955-6959. http://dx.doi.org/10.1073/pnas.82.20.6955
[13] Weisburg, W.G., Oyaizu, Y., Oyaizu, H. and Woese, C.R. (1985) Natural Relationship between Bacteroides and Flavobacteria. Journal of Bacteriology, 164, 230-236.                             eww150105lx
标签：

评论

发表评论

此博客中的热门博文

Does Immigration Promote the Investment of the Monopolistic Firm?

In the present paper, we examine the effect of increasing uncertainty of immigrants’ growth on the optimal timing of investment of a firm that has a monopolistic power over the labor market. It is revealed that when the uncertainty of immigrants’ growth is more than a threshold level, increasing uncertainty of immigrants’ growth accelerates the optimal timing of firms’ investment and enhances the economic growth, even if the uncertainty of immigrants’ growth is formulated by the geometric Brownian motion, which is in sharp contrast to the standard result that an increase in the uncertainty postpones the optimal timing. With an increase in the immigrants over the past ten years, workforces in the host countries have been growing significantly to the extent that the immigrants represent 70% of the increase in the workforce in Europe, and 47% in the United States as OECD indicates. In the present paper, we attempted to investigate the effect of increased uncertainty caused by the growi...
发表评论
阅读全文

Education Policy Implementation: A Mechanism for Enhancing Primary Education Development in Zanzibar

Education is one of the fundamental rights of individuals; therefore, the government of a country needs to develop and strengthen educational policy and quality as well as to ensure that everyone has equal access to basic education. The improvement of access and quality of education in the world is becoming as an essential factor in development, whereas the basic education (primary school), is acknowledged as a foundation of the higher educational development for every country. To fulfill this goal, governments introduce several policies and procedures; however, it requires some reforms and participation from the politician, policymakers, and other stakeholders to re-examine educational policy so that it can lead to multiplication and betterment of the reforms. Educational reforms actually focus on accountability. A positive educational development and reform is very challenging and needs more effort and strategy on how to use and utilize the resources effectively as such it can achie...
发表评论
阅读全文