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Expression of Messenger RNA of Oncoproteins E6 and E7 of Human Papilomavirus in Women with Negative Oncotic Cytologies, Epithelial Squamous Atypias of Undefined Significance and Low-Grade Intraepithelial Lesions

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http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52945#.VK3YEsnQrzE

Author(s) 
Daiene Carvalho Casagrande, Julisa Chamorro Lascasas Ribalta, Katia de Araujo Prado Ferreira Leite, Manoel Schimidt, Neila Maria de Góis Speck*

Affiliation(s)
Department of Gynecology, Escola Paulista de Medicina (UNIFESP), Sao Paulo, Brazil.

ABSTRACT
Objectives: To verify the prevalence of E6/E7 RNAm expression of HPV in patients with negative cervicovaginal cytology, ASC-US and LSIL; to correlate with negative anatomopathological exams and/or squamous intraepithelial neoplasy grade I (SIN 1) of the lower genital tract (LGT); to relate the RNAm expression with viral infection types; to assess the genotyping in single infections. Methods: Findings from 825 women submitted to E6/E7 RNAm survey and 422 women submitted to LGT biopsies were analyzed. Results: A larger percentage of E6/E7 in ASC-US and LSIL cytologies occurred. Negative results of RNAm expression were in accordance with negative cytologies and negative anatomopathological exam. In positive cases, the infection by a single HPV type was most prevalent, with the type 16 being the most common. Conclusions: the expression of mRNA was most prevalent in ASC-US and LSIL cytologies, comparing with the negative ones. The findings of SIN 1 biopsies were related to the positive expression of mRNA and negative cytologies; the negative expression was in agreement with negative anatomopathological exam. The infection by a HPV type was more frequent in cases of positive expression, the HPV type 16 being most frequently found. Patients with low grade intraepithelial lesion cytologies had a higher percentage of multiple infections.

KEYWORDS
E6/E7 RNAm, Human Papillomavirus, Negative Cytology, ASC-US Cytology, LSIL Cytology

Cite this paper
Casagrande, D. , Ribalta, J. , Leite, K. , Schimidt, M. and Speck, N. (2015) Expression of Messenger RNA of Oncoproteins E6 and E7 of Human Papilomavirus in Women with Negative Oncotic Cytologies, Epithelial Squamous Atypias of Undefined Significance and Low-Grade Intraepithelial Lesions. Open Journal of Obstetrics and Gynecology, 5, 36-43. doi: 10.4236/ojog.2015.51006.

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