跳至主要内容

Evidence of Synergistic Activity of Medicinal Plant Extracts against Neuraminidase Inhibitor Resistant Strains of Influenza Viruses

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52822#.VKn3mMnQrzE

The frequent emergence of drug resistant influenza viral strains emphasizes the urgent and continual need to develop new antiviral drugs. Given the encouraging findings of previous studies on antiviral compounds from plant sources, this study focused on medicinal plants from Borneo that were traditionally used to treat symptoms of influenza infection. Following the promising results of earlier investigations, four plant extracts that demonstrated multiple modes of viral inhibition were studied against wild-type and neuraminidase (NA) inhibitor-resistant strains of Types A and B influenza viruses. The extracts exhibited more pronounced activities against the wild-type viruses than the NA inhibitor-resistant strains. Variations in the antiviral potential of the extracts collected from different parts of the same plant were also evidenced in the in vitro micro-inhibition assays. Even though all plant extracts affected NA activity of all viruses, only two extracts demonstrated hemagglutination inhibitory (HI) activities against Type A pandemic H1N1 and Type B viruses. Furthermore, Receptor Destroying Enzyme (RDE) treatments of extracts exhibiting HI activities indicated the presence of sialic acid (SA)-like component(s) that may be responsible for HI activity. Since the antiviral potential of extracts was not completely suppressed by RDE, the possibility of non SA-like antiviral components cannot be ruled out. Therefore, synergistic activity between SA-like and non SA-like components contained in the plant extracts may be responsible for the demonstrated antiviral potential. The results also indicated the presence of non SA-like components that may act against other viral proteins apart from hemagglutinin (HA) and NA. Hence, this study supports the presence of multiple antiviral components that act against different viral proteins or interfere with different stages of viral replication. Our results suggest that these plant extracts have the potential to be developed as therapeutic agents for the treatment of influenza and could be a solution to the global occurrence of viral strains resistant to NA inhibitors.
Cite this paper
Rajasekaran, D. , Palombo, E. , Yeo, T. , Ley, D. , Tu, C. , Malherbe, F. and Grollo, L. (2014) Evidence of Synergistic Activity of Medicinal Plant Extracts against Neuraminidase Inhibitor Resistant Strains of Influenza Viruses. Advances in Microbiology, 4, 1260-1277. doi: 10.4236/aim.2014.416136.
 

[1] McCaughey, C. (2010) Influenza: A Virus of Our Times. Ulster Medical Journal, 79, 46-51.
[2] Tao, Z., et al. (2013) Complementary and Alternative Medicine Is Expected to Make Greater Contribution in Controlling the Prevalence of Influenza. BioScience Trends, 7, 253-256.
[3] Liao, Q., et al. (2013) Germacrone Inhibits Early Stages of Influenza Virus Infection. Antiviral Research, 100, 578-588. http://dx.doi.org/10.1016/j.antiviral.2013.09.021
[4] Presti, R.M., et al. (2009) Quaranfil, Johnston Atoll, and Lake Chad Viruses Are Novel Members of the Family Orthomyxoviridae. Journal of Virology, 83, 11599-11606.
http://dx.doi.org/10.1128/JVI.00677-09
[5] Nguyen, T.N.A., et al. (2011) Influenza A (H1N1) Neuraminidase Inhibitors from Vitis amurensis. Food Chemistry, 124, 437-443. http://dx.doi.org/10.1016/j.foodchem.2010.06.049
[6] Bouvier, N.M. and Palese, P. (2008) The Biology of Influenza Viruses. Vaccine, 26, D49-D53.
http://dx.doi.org/10.1016/j.vaccine.2008.07.039
[7] Krauss, S., et al. (2007) Influenza in Migratory Birds and Evidence of Limited Intercontinental Virus Exchange. PLOS Pathogens, 3, e167. http://dx.doi.org/10.1371/journal.ppat.0030167
[8] Tong, S., et al. (2012) A Distinct Lineage of Influenza A Virus from Bats. Proceedings of the National Academy of Sciences of the United States of America, 109, 4269-4274.
http://dx.doi.org/10.1073/pnas.1116200109
[9] Chen, J. and Deng, Y.M. (2009) Influenza Virus Antigenic Variation, Host Antibody Production and New Approach to Control Epidemics. Virology Journal, 6.
[10] Pleschka, S., et al. (2009) Anti-Viral Properties and Mode of Action of Standardized Echinacea Purpurea Extract against Highly Pathogenic Avian Influenza Virus (H5N1, H7N7) and Swine-Origin H1N1 (S-OIV). Virology Journal, 6.
[11] Robertson, J.S. and Inglis, S.C. (2011) Prospects for Controlling Future Pandemics of Influenza. Virus Research, 162, 39-46. http://dx.doi.org/10.1016/j.virusres.2011.09.024
[12] Ives, J.A.L., Carr, J.A., Mendel, D.B., Tai, C.Y., Lambkin, R., Kelly, L., et al. (2002) The H274Y Mutation in the Influenza A/H1N1 Neuraminidase Active Site Following Oseltamivir Phosphate Treatment Leave Virus Severely Compromised both in Vitro and in Vivo. Antiviral Research, 55, 307-317.
http://dx.doi.org/10.1016/S0166-3542(02)00053-0
[13] Samson, M., Pizzorno, A., Abed, Y. and Boivin, G. (2013) Influenza Virus Resistance to Neuraminidase Inhibitors. Antiviral Research, 98, 174-185. http://dx.doi.org/10.1016/j.antiviral.2013.03.014
[14] McKimm-Breschkin, J.L. (2013) Influenza Neuraminidase Inhibitors: Antiviral Action and Mechanisms of Resistance. Influenza and Other Respiratory Viruses, 7, 25-36. http://dx.doi.org/10.1111/irv.12047
[15] Ginting, T.E., Shinya, K., Kyan, Y., Makino, A., Matsumoto, N., Kaneda, S. and Kawaoka, Y. (2012) Amino Acid Changes in Hemagglutinin Contribute to the Replication of Oseltamivir-Resistant H1N1 Influenza Viruses. Journal of Virology, 86, 121-127. http://dx.doi.org/10.1128/JVI.06085-11
[16] Clercq, E.D. (2013) Highlights in Antiviral Drug Research: Antivirals at the Horizon. Medicinal Research Reviews, 33, 1215-1248.
[17] Rajasekaran, D., Palombo, E.A., Yeo, T.C., Siok Ley, D.L., Tu, C.L., Malherbe, F. and Grollo, L. (2013) Identification of Traditional Medicinal Plant Extracts with Novel Anti-Influenza Activity. PLoS ONE, 8, e79293. http://dx.doi.org/10.1371/journal.pone.0079293
[18] Chai, P.P.K. (2006) Medicinal Plants of Sarawak. Paul Chai P.K.
[19] Focho, D.A., Egbe, E.A., Chuyong, G.B., Fongod, A.G.N., Fonge, B.A., Ndam, W.T. and Youssoufa, B.M. (2010) An Ethnobotanical Investigation of the Annonaceae on Mount Cameroon. Journal of Medicinal Plants Research, 4, 2148-2158.
[20] Maji, S., Dandapat, P., Ojha, D., Maity, C., Halder, S.K., Das, P.K., et al. (2010) In Vitro Antimicrobial Potentialities of Different Solvent Extracts of Ethnomedicinal Plants against Clinically Isolated Human Pathogens. Journal of Phytology, 2, 57-64.
[21] Salleh, K.M., Latiff, A. and Malaysia. Kementerian Sains, T.d.A.S. (2002) Tumbuhan ubatan Malaysia. Pusat Pengurusan Penyelidikan, Universiti Kebangsaan Malaysia.
[22] Yaacob, M., Kadir, A.A. and Hasan, Z. (2009) Tumbuhan Ubatan Popular Malaysia. Institut Penyelidikan dan Kemajuan Pertanian Malaysia (MARDI).
[23] Oh, D.Y., Barr, I.G., Mosse, J.A. and Laurie, K.L. (2008) MDCK SIAT-1 Cells Show Improved Isolation Rates for Recent Human Influenza Viruses Compared to Conventional MDCK Cells. Journal of Clinical Microbiology, 46, 2189-2194.
[24] Reed, L.J. and Muench, H. (1938) A Simple Method of Estimating Fifty Percent Endpoints. American Journal of Epidemiology, 27, 493-497.
[25] Pourianfar, H.R., Javadi, A. and Grollo, L. (2012) A Colorimetric-Based Accurate Method for the Determination of Enterovirus 71 Titer. Indian Journal of Virology, 23, 303-310.
[26] Pourianfar, H.R., Poh, C.L., Fecondo, J. and Grollo, L. (2012) In Vitro Evaluation of the Antiviral Activity of Heparan Sulfate Mimetic Compounds against Enterovirus 71. Virus Research, 169, 22-29.
http://dx.doi.org/10.1016/j.virusres.2012.06.025
[27] Chen, D.Y., Shien, J.H., Tiley, L., Chiou, S.S., Wang, S.Y., Chang, T.J., et al. (2010) Curcumin Inhibits Influenza Virus Infection and Haemagglutination Activity. Food Chemistry, 119, 1346-1351.
http://dx.doi.org/10.1016/j.foodchem.2009.09.011
[28] WHO (2002) WHO Animal Influenza Manual.
http://www.who.int/vaccine_research/diseases/influenza/WHO_manual_on_animal-diagnosis_and_ surveillance_2002_5.pdf
[29] Grienke, U., Schmidtke, M., von Grafenstein, S., Kirchmair, J., Liedl, K.R. and Rollinger, J.M. (2012) Influenza Neuraminidase: A Druggable Target for Natural Products. Natural Product Reports, 29, 11-36. http://dx.doi.org/10.1039/c1np00053e
[30] Chang, T.T., Sun, M.F., Chen, H.Y., Tsai, F.J. and Chen, C.Y.C. (2011) Drug Design for Hemagglutinin: Screening and Molecular Dynamics from Traditional Chinese Medicine Database. Journal of the Taiwan Institute of Chemical Engineers, 42, 563-571. http://dx.doi.org/10.1016/j.jtice.2010.11.001
[31] Sarawak Biodiversity Centre (2012) Traditional Knowledge Documentation Programme Database. Kuching.
[32] Guo, C.T., Sun, X.L., Kanie, O., Shortridge, K.F., Suzuki, T., Miyamoto, D., et al. (2002) An O-Glycoside of Sialic Acid Derivative That Inhibits Both Hemagglutinin and Sialidase Activities of Influenza Viruses. Glycobiology, 12, 183-190. http://dx.doi.org/10.1093/glycob/12.3.183
[33] Matsubara, T., Onishi, A., Saito, T., Shimada, A., Inoue, H., Taki, T., et al. (2010) Sialic Acid-Mimic Peptides as Hemagglutinin Inhibitors for Anti-Influenza Therapy. Journal of Medicinal Chemistry, 53, 4441-4449. http://dx.doi.org/10.1021/jm1002183
[34] Racaniello, V. (2013) Virology Blog. Influenza 101. 5 November 2013.
http://www.virology.ws/2013/11/05/the-neuraminidase-of-influenza-virus/
[35] Federspiel, M., Fischer, R., Hennig, M., Mair, H.J., Oberhauser, T., Rimmler, G., et al. (1999) Industrial Synthesis of the Key Precursor in the Synthesis of the Anti-Influenza Drug Oseltamivir Phosphate (Ro 64-0796/002, GS-4104-02): Ethyl (3R,4S,5S)-4,5-epoxy-3-(1-ethyl-propoxy)-cyclohex-1-ene-1-carbo-xylate. Organic Process Research & Development, 3, 266-274.
http://dx.doi.org/10.1021/op9900176
[36] Kiyohara, H., Ichino, C., Kawamura, Y., Nagai, T., Sato, N. and Yamada, H. (2011) Patchouli Alcohol: In Vitro Direct Anti-Influenza Virus Sesquiterpene in Pogostemon cablin Benth. Journal of Natural Medicines, 66, 55-61.
[37] Kim, K.B., Kim, S.I. and Song, K.S. (2003) Neuraminidase Inhibitors from Mushroom Microphorus Affinis. Journal of Microbiology and Biotechnology, 13, 778-782.
[38] Yang, Z.C., Wang, B.C., Yang, X.S. and Wang, Q. (2005) Chemical Composition of the Volatile Oil from Cynanchum stauntonii and Its Activities of Anti-Influenza Virus. Colloids and Surfaces B: Biointerfaces, 43, 198-202.
http://dx.doi.org/10.1016/j.colsurfb.2005.05.003
[39] ISIRV (2012) Panel of Influenza A and B Viruses for Assessment of Neuraminidase Inhibitor Susceptibility. http://www.isirv.org/site/images/stories/avg_documents/Resistance/avg%20leaflet%20nov12.pdf eww150105lx
标签：

评论

发表评论

此博客中的热门博文

Does Immigration Promote the Investment of the Monopolistic Firm?

In the present paper, we examine the effect of increasing uncertainty of immigrants’ growth on the optimal timing of investment of a firm that has a monopolistic power over the labor market. It is revealed that when the uncertainty of immigrants’ growth is more than a threshold level, increasing uncertainty of immigrants’ growth accelerates the optimal timing of firms’ investment and enhances the economic growth, even if the uncertainty of immigrants’ growth is formulated by the geometric Brownian motion, which is in sharp contrast to the standard result that an increase in the uncertainty postpones the optimal timing. With an increase in the immigrants over the past ten years, workforces in the host countries have been growing significantly to the extent that the immigrants represent 70% of the increase in the workforce in Europe, and 47% in the United States as OECD indicates. In the present paper, we attempted to investigate the effect of increased uncertainty caused by the growi...
发表评论
阅读全文

Education Policy Implementation: A Mechanism for Enhancing Primary Education Development in Zanzibar

Education is one of the fundamental rights of individuals; therefore, the government of a country needs to develop and strengthen educational policy and quality as well as to ensure that everyone has equal access to basic education. The improvement of access and quality of education in the world is becoming as an essential factor in development, whereas the basic education (primary school), is acknowledged as a foundation of the higher educational development for every country. To fulfill this goal, governments introduce several policies and procedures; however, it requires some reforms and participation from the politician, policymakers, and other stakeholders to re-examine educational policy so that it can lead to multiplication and betterment of the reforms. Educational reforms actually focus on accountability. A positive educational development and reform is very challenging and needs more effort and strategy on how to use and utilize the resources effectively as such it can achie...
发表评论
阅读全文