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http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53000#.VK9BqsnQrzE
Author(s)
Diego J. S. Dias1,2, Graziella A. Joanitti3, Ricardo B. Azevedo2, Luciano P. Silva4, Claure N. Lunardi1,3, Anderson J. Gomes1,3*
Affiliation(s)
1School of Health Sciences, University of Brasilia, Brasilia, Brazil.
2Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
3Laboratory of Nanobiotechnology, Faculty of Ceilandia, University of Brasilia, Brasilia, Brazil.
4Laboratory of Mass Spectrometry, EMBRAPA, Genetic Resources and Biotechnology, Brasilia, Brazil.
2Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
3Laboratory of Nanobiotechnology, Faculty of Ceilandia, University of Brasilia, Brasilia, Brazil.
4Laboratory of Mass Spectrometry, EMBRAPA, Genetic Resources and Biotechnology, Brasilia, Brazil.
ABSTRACT
The
present work aimed to develop and evaluate a colloidal system composed
of poly (DL-lactide-co-glycolide) (PLGA) nanoparticles (NPs) associated
with chlorambucil (CHB) and its effects on cancer cells. The
nanoparticles showed %EE (>92%), a mean particle size in the range of
240 to 334 nm and zeta potential of -16.7 to -26.0 mV. In vitro release
profile showed a biphasic pattern, with an initial burst for all
formulations. The scanning electron microscopy of CHB-nanoparticles
showed regular spherical shapes, smooth surface without aggregations.
Differential scanning calorimetry thermograms, UV-vis absorption,
fluorescence emission and Fourier transform infrared spectroscopy were
performed showing the entrapment of the antitumoral in drug delivery
system. CHB encapsulated in PLGA nanoparticles decrease the survival
rates of the breast cancer cells: 68.9% reduction of cell viability on
MCF-7 cell line and 59.7% on NIH3T3. Our results indicated that
polymeric nanoparticles produced by classical methods are efficient drug
delivery systems for CHB.
Cite this paper
References
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