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Application of a Rapid ESI-MS/MS Method for Quantitative Analysis of Docetaxel in Polymeric Matrices of PLGA and PLGA-PEG Nanoparticles through Direct Injection to Mass Spectrometer

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ABSTRACT
Docetaxel is a member of taxan family of antineoplastic agents widely used in cancer chemotherapy. However, application of conventional chemotherapy with commercial formulation has been accompanied with matters of concern regarding drug’s biodistribution, pharmacokinetics, and pharmacodynamics. Polymeric nanoparticles have been widely used as unique drug delivery vehicles to circumvent such problems. Docetaxel-loaded poly (lactide-co-glycolide) (PLGA) and poly (lactide-co-glycolide)-poly (ethylene glycol) (PLGA-PEG) nanoparticles fit well in modifying drug’s pharmacokinetic characteristics as intravenous (IV) sustained-release delivery vehicles. In such circumstances, characterization of nanoparticles in terms of their drug-payload would be a necessary step. The majority of studies have used HPLC analysis method for docetaxel quantitation in polymeric nanoparticles. Herein, a rapid ESI-MS/MS method for quantitative analysis of docetaxel in polymeric matrices of PLGA and PLGA-PEG nanoparticles through direct injection to mass spectrometer has been developed and validated. The assay was validated over a range of 3.9 - 1000 ng/ml and 125 - 16,000 ng/ml. Samples were directly injected to the instrument through an isocratic elution (0.1% formic acid in methanol) and detection was performed on a Hybrid Triple Quadrupole/Linear Ion trap mass spectrometer with multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI) source. The run time and retention time were 2 and 0.6 minutes respectively. The method demonstrated acceptable level of accuracy and precision and was successfully applied for quantitative analysis of docetaxel in polymeric nanoparticles of PLGA and PLGA-PEG.
 
Cite this paper
Rafiei, P. , Michel, D. and Haddadi, A. (2015) Application of a Rapid ESI-MS/MS Method for Quantitative Analysis of Docetaxel in Polymeric Matrices of PLGA and PLGA-PEG Nanoparticles through Direct Injection to Mass Spectrometer. American Journal of Analytical Chemistry, 6, 164-175. doi: 10.4236/ajac.2015.62015.
 
References
[1]Herbst, R.S. and Khuri, F.R. (2003) Mode of Action of Docetaxel—A Basis for Combination with Novel Anticancer Agents. Cancer Treatment Reviews, 29, 407-415.
http://dx.doi.org/10.1016/S0305-7372(03)00097-5
 
[2]Takimoto, C. and Beeram, M. (2008) Microtubule Stabilizing Agents in Clinical Oncology, the Taxanes. In: Fojo, T., Ed., The Role of Microtubules in Cell Biology, Neurobiology, and Oncology, Human Press, Totowa, 395-419.
http://dx.doi.org/10.1007/978-1-59745-336-3_16
 
[3]Hitt, R. and Rodríguez, C. (2011) Docetaxel. In: Schwab, M., Ed., Encyclopedia of Cancer, Springer-Verlag, Berlin, Heidelberg, 1148-1150.
 
[4]Zhao, P. and Astruc, D. (2012) Docetaxel Nanotechnology in Anticancer Therapy. ChemMedChem, 7, 952-972.
http://dx.doi.org/10.1002/cmdc.201200052
 
[5]van Zuylen, L., Verweij, J. and Sparreboom, A. (2001) Role of Formulation Vehicles in Taxane Pharmacology. Investigational New Drugs, 19, 125-141.
http://dx.doi.org/10.1023/A:1010618632738
 
[6]ten Tije, A.J., Verweij, J., Loos, W.J. and Sparreboom, A. (2003) Pharmacological Effects of Formulation Vehicles: Implications for Cancer Chemotherapy. Clinical Pharmacokinetics, 42, 665-685.
http://dx.doi.org/10.2165/00003088-200342070-00005
 
[7]Blanco, E., Hsiao, A., Mann, A.P., Landry, M.G., Meric-Bernstam, F. and Ferrari, M. (2011) Nanomedicine in Cancer Therapy: Innovative Trends and Prospects. Cancer Science, 102, 1247-1252.
http://dx.doi.org/10.1111/j.1349-7006.2011.01941.x
 
[8]Jabir, N.R., Tabrez, S., Ashraf, G.M., Shakil, S., Damanhouri, G.A. and Kamal, M.A. (2012) Nanotechnology-Based Approaches in Anticancer Research. International Journal of Nanomedicine, 7, 4391-4408.
http://dx.doi.org/10.2147/IJN.S33838
 
[9]Dinarvand, R., Sepehri, N., Manoochehri, S., Rouhani, H. and Atyabi, F. (2011) Polylactide-co-Glycolide Nanoparticles for Controlled Delivery of Anticancer Agents. International Journal of Nanomedicine, 6, 877-895.
http://dx.doi.org/10.2147/ijn.s18905
 
[10]Hamidi, M., Azadi, A. and Rafiei, P. (2006) Pharmacokinetic Consequences of Pegylation. Drug Delivery, 13, 399-409.
http://dx.doi.org/10.1080/10717540600814402
 
[11]Hamidi, M., Rafiei, P. and Azadi, A. (2008) Designing PEGylated Therapeutic Molecules: Advantages in ADMET Properties. Expert Opinion on Drug Discovery, 3, 1293-1307.
http://dx.doi.org/10.1517/17460441.3.11.1293
 
[12]Esmaeili, F., Dinarvand, R., Ghahremani, M.H., Ostad, S.N., Esmaily, H. and Atyabi, F. (2010) Cellular Cytotoxicity and in-Vivo Biodistribution of Docetaxelpoly(lactide-co-glycolide) Nanoparticles. Anticancer Drugs, 21, 43-52.
http://dx.doi.org/10.1097/CAD.0b013e328331f934
 
[13]Musumeci, T., Ventura, C.A., Giannone, I., Ruozi, B., Montenegro, L., Pignatello, R. and Puglisi, G. (2006) PLA/PLGA Nanoparticles for Sustained Release of Docetaxel. International Journal of Pharmaceutics, 325, 172-179.
http://dx.doi.org/10.1016/j.ijpharm.2006.06.023
 
[14]Sanna, V., Roggio, A.M., Posadino, A.M., Cossu, A., Marceddu, S., Mariani, A., Alzari, V., Uzzau, S., Pintus, G. and Sechi, M. (2011) Novel Docetaxel-Loaded Nanoparticles Based on Poly(lactide-co-caprolactone) and Poly(lactide-coglycolide-co-caprolactone) for Prostate Cancer Treatment: Formulation, Characterization, and Cytotoxicity Studies. Nanoscale Research Letters, 6, 260.
http://dx.doi.org/10.1186/1556-276X-6-260
 
[15]Senthilkumar, M., Mishra, P. and Jain, N.K. (2008) Long Circulating PEGylatedpoly(D,L-lactide-co-glycolide) Nanoparticulate Delivery of Docetaxel to Solid Tumors. Journal of Drug Targeting, 16, 424-435.
http://dx.doi.org/10.1080/10611860802088598
 
[16]Danhier, F., Lecouturier, N., Vroman, B., Jérôme, C., Marchand-Brynaert, J., Feron, O. and Préat, V. (2009) Paclitaxel-Loaded PEGylated PLGA-Based Nanoparticles: in Vitro and in Vivo Evaluation. Journal of Controlled Release, 133, 11-17.
http://dx.doi.org/10.1016/j.jconrel.2008.09.086
 
[17]Cheng, J., Teply, B.A., Sherifi, I., Sung, J., Luther, G., Gu, F.X., Levy-Nissenbaum, E., Radovic-Moreno, A.F., Langer, R. and Farokhzad, O.C. (2007) Formulation of Functionalized PLGA-PEG Nanoparticles for in Vivo Targeted Drug Delivery. Biomaterials, 28, 869-876.
http://dx.doi.org/10.1016/j.biomaterials.2006.09.047
 
[18]Dhankar, R., Rathee, P., Jain, A.K., Arora, S., Senthilkumar, M., Rath, G., Kumarsaxena, A., Sharma, P.R., Chashoo, G. and Goyal, A.K. (2011) HER-2 Targeted Immunonanoparticles for Breast Cancer Chemotherapy. Journal of Applied Pharmaceutical Science, 1, 132-139.
 
[19]López, L.Z., Pastor, A.A., Beitia, A., Manuel, J., Velilla, J.A. and Deiró, J.G. (2006) Determination of Docetaxel and Paclitaxel in Human Plasma by High-Performance Liquid Chromatography: Validation and Application to Clinical Pharmacokinetic Studies. Therapeutic Drug Monitoring, 28, 199-205.
http://dx.doi.org/10.1097/01.ftd.0000189903.46802.1f
 
[20]Bermingham, S., O’Connor, R., Regan, F. and McMahon, G.P. (2010) Simultaneous Determination of Anthracyclines and Taxanes in Human Serum Using Online Sample Extraction Coupled to High Performance Liquid Chromatography with UV Detection. Journal of Separation Science, 33, 1571-1579.
http://dx.doi.org/10.1002/jssc.201000026
 
[21]Parise, R.A., Ramanathan, R.K., Zamboni, W.C. and Egorin, M.J. (2003) Sensitive Liquid Chromatography-Mass Spectrometry Assay for Quantitation of Docetaxel and Paclitaxel in Human Plasma. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 783, 231-236.
http://dx.doi.org/10.1016/S1570-0232(02)00659-1
 
[22]Wang, L.Z., Goh, B.C., Grigg, M.E., Lee, S.C., Khoo, Y.M. and Lee, H.S. (2003) A Rapid and Sensitive Liquid Chromatography/Tandem Mass Spectrometry Method for Determination of Docetaxel in Human Plasma. Rapid Communications in Mass Spectrometry, 17, 1548-1552.
http://dx.doi.org/10.1002/rcm.1091
 
[23]Hou, W., Watters, J.W. and McLeod, H.L. (2004) Simple and Rapid Docetaxel Assay in Plasma by Protein Precipitation and High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 804, 263-267.
http://dx.doi.org/10.1016/j.jchromb.2004.01.021
 
[24]Mortier, K.A., Renard, V., Verstraete, A.G., Van Gussem, A., Van Belle, S. and Lambert, W.E. (2005) Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Assay for the Quantification of Docetaxel and Paclitaxel in Human Plasma and Oral Fluid. Analytical Chemistry, 77, 4677-4683.
http://dx.doi.org/10.1021/ac0500941
 
[25]Guitton, J., Cohen, S., Tranchand, B., Vignal, B., Droz, J.P., Guillaumont, M., Manchon, M. and Freyer, G. (2005) Quantification of Docetaxel and Its Main Metabolites in Human Plasma by Liquid Chromatography/Tandem Mass Spectrometry. Rapid Communications in Mass Spectrometry, 19, 2419-2426.
http://dx.doi.org/10.1002/rcm.2072
 
[26]Huang, Q., Wang, G.J., Sun, J.G., Hu, X.L., Lu, Y.H. and Zhang, Q. (2007) Simultaneous Determination of Docetaxel and Ketoconazole in Rat Plasma by Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry. Rapid Communications in Mass Spectrometry, 21, 1009-1018.
http://dx.doi.org/10.1002/rcm.2903
 
[27]Gustafson, D.L., Long, M.E., Zirrolli, J.A., Duncan, M.W., Holden, S.N., Pierson, A.S. and Eckhardt, S.G. (2003) Analysis of Docetaxel Pharmacokinetics in Humans with the Inclusion of Later Sampling Time-Points Afforded by the Use of a Sensitive Tandem LCMS Assay. Cancer Chemotherapy and Pharmacology, 52, 159-166.
http://dx.doi.org/10.1007/s00280-003-0622-z
 
[28]Baker, S.D., Sparreboom, A. and Verweij, J. (2006) Clinical Pharmacokinetics of Docetaxel: Recent Developments. Clinical Pharmacokinetics, 45, 235-252.
http://dx.doi.org/10.2165/00003088-200645030-00002
 
[29]Kuppens, I.E., van Maanen, M.J., Rosing, H., Schellens, J.H. and Beijnen. J.H. (2005) Quantitative Analysis of Docetaxel in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry. Biomedical Chromatography, 19, 355-361.
http://dx.doi.org/10.1002/bmc.457
 
[30]Sparreboom, A., Zhao, M., Brahmer, J.R., Verweij, J. and Baker, S.D. (2002) Determination of the Docetaxel Vehicle, Polysorbate 80, in Patient Samples by Liquid Chromatography-Tandem Mass Spectrometry. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 773, 183-190.
http://dx.doi.org/10.1016/S1570-0232(02)00167-8
 
[31]Ito, Y., Goto, T., Oka, H., Matsumoto, H., Miyazaki, Y., Takahashi, N. and Nakazawa, H. (2003) Simple and Rapid Determination of Thiabendazole, Imazalil, and O-Phenylphenol in Citrus Fruit Using Flow-Injection Electrospray Ionization Tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry, 51, 861-866.
http://dx.doi.org/10.1021/jf020809q
 
[32]Song, F., El-Demerdash, A., Lee, S.J. and Smith, R.E. (2012) Fast Screening of Lovastatin in Red Yeast Rice Products by Flow Injection Tandem Mass Spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 57, 76-81.
http://dx.doi.org/10.1016/j.jpba.2011.08.039
 
[33]Zhou, H., Tai, Y., Sun, C. and Pan, Y. (2005) Rapid Identification of Vinca Alkaloids by Direct-Injection Electrospray Ionisation Tandem Mass Spectrometry and Confirmation by High-Performance Liquid Chromatography-Mass Spectrometry. Phytochemical Analysis, 16, 328-333.
http://dx.doi.org/10.1002/pca.852
 
[34]Carducci, C., Santagata, S., Leuzzi, V., Carducci, C., Artiola, C., Giovanniello, T., Battini, R. and Antonozzi, I. (2006) Quantitative Determination of Guanidinoacetate and Creatine in Dried Blood Spot by Flow Injection Analysis Electrospray Tandem Mass Spectrometry. Clinica Chimica Acta, 364, 180-187.
http://dx.doi.org/10.1016/j.cca.2005.06.016
 
[35]Peer, C.J., Clay, D.J., Glover, H.L., Renninger, K.L., Kraner, J.C. and Callery, P.S. (2008) Direct Injection Mass Spectrometric Confirmation of Multiple Drugs in Overdose Cases from Postmortem Blood Using Electrospray Ionization-Tandem Mass Spectrometry and MS(3). Journal of Analytical Toxicology, 32, 709-714.
http://dx.doi.org/10.1093/jat/32.8.709
 
[36]Peer, C.J., Shakleya, D.M., Younis, I.R., Kraner, J.C. and Callery, P.S. (2007) Direct-Injection Mass Spectrometric Method for the Rapid Identification of Fentanyl and Norfentanyl in Postmortem Urine of Six Drug-Overdose Cases. Journal of Analytical Toxicology, 31, 515-521.
http://dx.doi.org/10.1093/jat/31.8.515
 
[37]Grozav, A.G., Hutson, T.E., Zhou, X., Bukowski, R.M., Ganapathi, R. and Xu, Y. (2004) Rapid Analysis of Docetaxel in Human Plasma by Tandem Mass Spectrometry with On-Line Sample Extraction. Journal of Pharmaceutical and Biomedical Analysis, 36, 125-131.
http://dx.doi.org/10.1016/j.jpba.2004.05.014
 
[38]Yamaguchi, H., Fujikawa, A., Ito, H., Tanaka, N., Furugen, A., Miyamori, K., Takahashi, N., Ogura, J., Kobayashi, M., Yamada, T., Mano, N. and Iseki, K. (2012) A Rapid and Sensitive LC/ESI-MS/MS Method for Quantitative Analysis of Docetaxel in Human Plasma and Its Application to a Pharmacokinetic Study. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 893-894, 157-161.
http://dx.doi.org/10.1016/j.jchromb.2012.02.003
 
[39]Corona, G., Elia, C., Casetta, B., Frustaci, S. and Toffoli, G. (2011) High-Throughput Plasma Docetaxel Quantification by Liquid Chromatography-Tandem Mass Spectrometry. Clinica Chimica Acta, 412, 358-364.
http://dx.doi.org/10.1016/j.cca.2010.11.010
 
[40]Keum, C.G., Noh, Y.W., Baek, J.S., Lim, J.H., Hwang, C.J., Na, Y.G., Shin, S.C. and Cho, C.W. (2011) Practical Preparation Procedures for Docetaxel-Loaded Nanoparticles Using Polylactic Acid-Co-Glycolic Acid. International Journal of Nanomedicine, 6, 2225-2234.
http://dx.doi.org/10.2147/IJN.S24547
 
[41]Shi, W., Zhang, Z.J., Yuan, Y., Xing, E.M., Qin, Y., Peng, Z.J., Zhang, Z.P. and Yang, K.Y. (2013) Optimization of Parameters for Preparation of Docetaxel-Loaded PLGA Nanoparticles by Nanoprecipitation Method. Journal of Huazhong University of Science and Technology, 33, 754-758.
http://dx.doi.org/10.1007/s11596-013-1192-x                                                                          eww150122lx
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