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Micropropagation of Kebericho: An Endandered Ethiopian Medicinal Plant

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http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52514#.VJor_cCAM4


Echinops kebericho, endemic to Ethiopia, is a critically endangered medicinal plant. It is among the most important medicinal plants of the country, valued primarily for its root parts. The commercial harvesting and sale of roots of E. kebericho have threatened local populations. This study aimed to develop micropropagation protocol for E. kebericho using shoot tip explants. The study started with seed germination test using seeds stored for different months. Shoot tips from in vitro germinated seedlings were cultured on shoot initiation MS media supplemented with 0, 0.1, 0.5, 1.0, 1.5 and 2.0 mg/l BAP or KN alone. Explants were cultured on shoot proliferation media fortified with Kinetin, BAP, and TDZ each at 0.0, 0.5, 1.0, 2.5, 3.0 and 5.0 mg/l either alone or in combination with 0.0, 0.1, 0.25 and 0.5 mg/l NAA. For rooting, full, half and 1/3 strength MS media supplemented with IBA and NAA alone each at 0, 0.05, 0.1, 0.5, 1.0, 1.5 and 2.5 mg/l were used. Growth regulator free MS medium was used as control. Study results showed that 100% germination was recorded in fresh seeds and dropped as low as 65.18% and 22.3% for 3 and 5 months seeds respectively. 1.0 mg/l KN and 0.5 mg/l KN + 0.1 mg/l NAA showed maximum shoot proliferation on shoot induction media and shoot multiplication media respectively. Best rooting was obtained on 1/3 MS containing 1.5 mg/l NAA with 8.23 roots and 4.82 cm root length and established under greenhouse with 83% survival.
Cite this paper
Manahlie, B. and Feyissa, T. (2014) Micropropagation of Kebericho: An Endandered Ethiopian Medicinal Plant. American Journal of Plant Sciences, 5, 3836-3847. doi: 10.4236/ajps.2014.526402. 
 

[1] Tesema, T., Mirutse, G., Negussu, A. and Teshome, H. (2003) Medicinal Plants Biodiversity. National Biodiversity Strategy and Action Plan (NBSAP) Project, IBCR, Addis Ababa.
[2] Getachew, B., Yinebeb, T., Taddesse, K., Ariaya, H. and Yalemtsehay, M. (2011) Ethnopharmacological Investigations of Essential Oils Isolated from Five Ethiopian Medicinal Plants against Eleven Pathogenic Bacterial Strains. Phytopharmacology, 1, 133-143.
[3] Hareya, F. (2003) Local Health Knowledge and Home-Based Medicinal Plant Use in Ethiopia. International Development Centre. Ph.D. Dissertation, Oxford University, Oxford.
[4] Dawit, A. and Ahadu, A. (1993) Medicinal Plants and Enigmatic Health Practices of Northern Ethiopia. Berhanena Selam Printing Press, Addis Ababa.
[5] Belachew, D. (1993) Ethiopian Traditional Herbal Drugs. Part II: Antimicrobial Activity of Medicinal Plants. Journal of Ethnopharmacology, 39, 129-139.
[6] Abegaz, B., Mutanyatta-Comar, J. and Nindi, M. (2007) Naturally Occurring Homoisoflavonoids: Phytochemistry, Biological Activities and Synthesis. Natural Product Communications, 2, 475-498.
[7] Tariku, Y., Hymete, A., Hailu, A. and Rohloff, J. (2011) In Vitro Evaluation of Antileishmanial Activity and Toxicity of Essential Oils of Artemisia absinthium and Echinops kebericho. Chemistry & Biodiversity, 8, 614-623. http://dx.doi.org/10.1002/cbdv.201000331
[8] Hymete, A., Rohloff, J., Iversen, T.H. and Kjøsen, H. (2007) Volatile Constituents of the Roots of Echinops kebericho Mesfin. Flavour and Fragrance Journal, 22, 35-38.
http://dx.doi.org/10.1002/ffj.1746
[9] Hymete, A. and Kidane, A. (1991) Screening for Anthelmintic Activity in Two Echinops spp. Ethiop. The Pharmaceutical Journal, 9, 67-71.
[10] Jemal, H., Kaba, U., Fayissa, R., Awol, J., Sultan, A. and Nazif, H. (2011) Antihelmentic Effects of the Essential Oil Extracts of Selected Medicinal Plants against Haemonchus contortus. International Journal of Agricultural Research, 6, 290-298. http://dx.doi.org/10.3923/ijar.2011.290.298
[11] Hymete, A. and Afifi, M.S. (1997) Investigation of the Fixed Oil Isolated from the Roots of Echinops kebericho Mesfin. Mansoura. Journal of Pharmacological Sciences, 13, 59-69.
[12] Abegaz, B. (1996) Progress in the Chemistry of Marketed Traditionally Used Plants of Ethiopia-Review Article. Bulletin of the Chemical Society of Ethiopia, 10, 57-71.
[13] Cunningham, A.B. (1993) African Medicinal Plants: Setting Priorities at the Interface between Conservation and Primary Health Care. UNESCO, Nairobi, People and Plants Working Paper 1.
[14] Mesfin, T. and Brook, M. (2010) A Review of Selected Plants Used in the Maintenance of Health and Wellness in Ethiopia. Ethiopian e-Journal for Research and Innovation Foresight, 2, 85-102.
[15] Reta, R. (2013) Assessment of Indigenous Knowledge of Medicinal Plant Practice and Mode of Service Delivery in Hawassa City, Southern Ethiopia. Journal of Medicinal Plants Research, 7, 517-535.
[16] Global Environment Facility (2012) Capacity Building for Access and Benefit Sharing and Conservation and Sustainable Use of Medicinal Plants. Ethiopia ABS CSUMP CEO Endorsement Document. www.theGEF.org
[17] Balcha, A., Legesse, N. and Jochen, K. (2009) In Vitro and ex Vitro Seed-Based Propagation Methods of Echinops kebericho Mesfin: A Threatened Medicinal Plant. Journal of Applied Horticulture, 10, 164-168.
[18] Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
[19] Kaewsuwan, S., Soonthornchareonnon, N. and Prathanturarug, S. (2005) In Vitro Propagation of Mallotus repandus (Willd.) Muell. Arg. Acta Horticulturae, 2, 95-101.
[20] Ali, A., Gull, I., Naz, S. and Afghan, S. (2010) Biochemical Investigation during Different Stages of in Vitro Propagation of Stevia rebaudiana. Pakistan Journal of Botany, 42, 2827-2837.
[21] Yan, H.B., Liang, C.X., Yang, L.T. and Li, Y.R. (2010) In Vitro and ex Vitro Rooting of Sratia grosvenorii, a Traditional Medicinal Plant. Acta Physiologiae Plantarum, 32, 115-120.
http://dx.doi.org/10.1007/s11738-009-0386-0
[22] Arunkumar, B.S. and Jayaraj, M. (2011) Effect of Auxins and Cytokinins on in Vitro Propagation of Ionidium suffruticosum Giing—A Seasonal Multipotent Medicinal Herb. International Journal of Research in Ayurveda and Pharmacy, 2, 198-203.
[23] Pandey, P., Mehta, R. and Adhyay, R. (2013) In Vitro Propagation of an Endangered Medicinal Plant Psoralea corylifolia Linn. Asian Journal of Pharmaceutical and Clinical Research, 6, 115-118.
[24] Pandiyan, P. and Selvaraj, T. (2012) In Vitro Multiplication of Bacopa monnieri (L.) Pennell from Shoot Tip and Nodal Explants. Journal of Agricultural Technology, 8, 1099-1108.
[25] Gopal, G.V., Repalle, S., Talluri, V.R., Ronda, S.R. and Allu, P.R. (2013) In Vitro Propagation and GC-MS Studies of Ocimum basilicum Linn. var. pilosum (Willd.) Benth. British Biotechnology Journal, 4, 96-107. http://dx.doi.org/10.9734/BBJ/2014/6225
[26] Rasool, R., Ganai, B.A., Kamili, A.N., Akbar, S. and Masood, A. (2013) Synergistic Effect of Auxins and Cytokinins on Propagation of Artemisia amygdalina (Asteraceae), a Critically Endangered Plant of Kashmir. Pakistan Journal of Botany, 45, 629-634.
[27] Joshi, M. and Dhar, U. (2003) In Vitro Propagation of Saussurea obvallata (DC.) Edgew.—An Endangered Ethnore- ligious Medicinal Herb of Himalaya. Plant Cell Reports, 21, 933-939. http://dx.doi.org/10.1007/s00299-003-0601-1
[28] Baskaran, P. and Jayabalan, N. (2005) An Efficient Micropropagation System for Eclipa alba—A Valuable Medicinal Herb. In Vitro Cellular & Developmental Biology-Plant, 41, 532-539. http://dx.doi.org/10.1079/IVP2005667
[29] Lu, M.C. (2005) Micropropagation of Vitis thunbergii Sieb. et Zucc., a Medicinal Herb, through High-Frequency Shoot Tip Culture. Scientia Horticulturae, 107, 64-69.
http://dx.doi.org/10.1016/j.scienta.2005.05.014
[30] Sharma, S. and Shahzad, A. (2013) Efficient Micropropagation of Spilanthes acmella (L.) Murr: A Threatened Medicinal Herb. British Biotechnology Journal, 3, 1-11.
[31] Aslam, N., Zia, M. and Chaudhary, M.F. (2006) Callogenesis and Direct Organogenesis of Artemisia scoparia. Pakistan Journal of Biological Sciences, 9, 1783-1786.
http://dx.doi.org/10.3923/pjbs.2006.1783.1786
[32] Patel, R.M. and Shah, R.R. (2009) Regeneration of Stevia Plant through Callus Culture. Indian Journal of Pharmaceutical Sciences, 71, 46-50. http://dx.doi.org/10.4103/0250-474X.51954
[33] Thomas, T.D. (2007) Pretreatment in Thidiazuron Improves the in Vitro Shoot Induction from Leaves in Curculigo orchioides Gaertn, an Endangered Medicinal Plant. Acta Physiologiae Plantarum, 29, 455-461. http://dx.doi.org/10.1007/s11738-007-0055-0
[34] Baker, C.M. and Wetzstein, H.Y. (2004) Influence of Auxin Type and Concentration on Peanut Somatic Embryogenesis. Plant Cell, Tissue and Organ Culture, 36, 361-368.
http://dx.doi.org/10.1007/BF00046094                         eww141224lx
[35] Kaldewey, H. (1984) Transport and Other Modes of Movements of Hormones (Mainly Auxins). In: Scott, T.K., Ed., Hormonal Regulation of Development II, Encyclopedia of Plant Physiology, New Series, Vol. 10, 80-148.
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