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Effects of mRNA, Protein Expression and Activity for Myocardial SOD2 by a Single Bout of or Long-Term Strenuous Endurance Exercise in Rats

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Objective: To explore the effects of myocardial SOD2 by strenuous endurance exercise. Methods: 27 grown male SD rats were randomly divided into control group (C), a single bout of strenuous endurance exercise group (E1) and seventh-week strenuous endurance exercise group (E2). Real-time PCR was used to observe the changes of mRNA expression for myocardial SOD2. Western bolt was used to observe the changes of SOD2 protein expression. In addition, SOD2, T-SOD and SOD1 activity changes were observed. Results: Myocardial SOD2 expression level at mRNA and protein of Group E1, E2 was significantly higher than that in group C, and SOD2 and T-SOD activity in group E2 were significantly higher than those in group C. Those changes were more obvious in group E2. Conclusions: Strenuous endurance exercise can improve level of myocardial SOD2 expression at mRNA and protein, and enhance the activity for SOD2, thus increasing the activity for T-SOD. Effect of long-term strenuous endurance exercise was better than a single bout of one.
Cite this paper
Xu, S. , Liu, W. and Li, M. (2014) Effects of mRNA, Protein Expression and Activity for Myocardial SOD2 by a Single Bout of or Long-Term Strenuous Endurance Exercise in Rats. Chinese Medicine, 5, 251-258. doi: 10.4236/cm.2014.54030. 
 

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