跳至主要内容

Effect of Ethanol Extract of Venenum Bufonis on Biofilm Formation of Staphylococcus aureus

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52415#.VJdpecCAM4

Author(s)
Ethanol extract of Venenum Bufonis has shown many valuable bioactivities, but little is known about its effect on biofilm formation of Staphylococcus aureus. In this study, biofilm formation of S. aureus NCTC8325 with or without ethanol extract of Venenum Bufonis was tested using microtitre plate assay and Confocal Laser Scanning Microscope (CLSM) system. Results show that the biofilm formation of S. aureus with ethanol extract of Venenum Bufonis was significantly lower than that of the control group without ethanol extract of Venenum Bufonis. Meanwhile, the reduction degree was correlated to the concentration of ethanol extract of Venenum Bufonis positively. With CLSM system we can observe that looser and less biomass of the biofilm structure of the experimental group appeared than that of the control group. These results suggested that ethanol extract of Venenum Bufonis has inhibitory effect on biofilm formation of S. aureus.
Cite this paper
Yan, B. , Guo, M. and Zhao, L. (2014) Effect of Ethanol Extract of Venenum Bufonis on Biofilm Formation of Staphylococcus aureus. Chinese Medicine, 5, 218-222. doi: 10.4236/cm.2014.54026. 
 

[1] Lowy, F.D. (1998) Staphylococcus aureus Infections. New England Journal of Medicine, 339, 520-532.
http://dx.doi.org/10.1056/NEJM199808203390806
[2] Otto, A., van Dijl, J.M., Hecker, M. and Becher, D. (2014) The Staphylococcus aureus Proteome. International Journal of Medical Microbiology, 304, 110-120.
http://dx.doi.org/10.1016/j.ijmm.2013.11.007
[3] Costerton, J.W., Montanaro, L. and Arciola, C.R. (2005) Biofilm in Implant Infections: Its Production and Regulation. The International Journal of Artificial Organs, 28, 1062-1068.
[4] Del Pozo, J.L. and Patel, R. (2007) The Challenge of Treating Biofilm-Associated Bacterial Infections. Clinical Pharmacology and Therapeutics, 82, 204-209.
http://dx.doi.org/10.1038/sj.clpt.6100247
[5] Archer, N.K., Mazaitis, M.J., Costerton, J.W., Leid, J.G., Powers, M.E. and Shirtliff, M.E. (2011) Staphylococcus aureus Biofilms: Properties, Regulation, and Roles in Human Disease. Virulence, 2, 445-459.
http://dx.doi.org/10.4161/viru.2.5.17724
[6] Wang, S.H., Wang, Y.C., Nie, Y.L., Hai, Y.N., Sun, H.F., Yuan, Z.L. and Nan, K.J. (2013) Antiproliferative Activity of the Chinese Medicinal Compound, Delisheng, Compared with Rg3 and Gemcitabine in HepG2 Cells. Indian Journal of Pharmaceutical Sciences, 75, 578-584.
[7] Chen, G., Jiang, X., Li, J., Duan, G., Yang, L., Zhang, Y. and Wang, F. (2014). Study on Anti-Osteosarcoma Activity of Ethanol Extract of Venenum Bufonis in Vitro. African Journal of Traditional, Complementary, and Alternative Medicines. African Networks on Ethnomedicines, 11, 73-77.
[8] Zhang, D.M., Liu, J.S., Tang, M.K., Yiu, A., Cao, H.H., Jiang, L., Chan, J.Y., Tian, H.Y., Fung, K.P. and Ye, W.C. (2012) Bufotalin from Venenum Bufonis Inhibits Growth of Multidrug Resistant HepG2 Cells through G2/M Cell Cycle Arrest and Apoptosis. European Journal of Pharmacology, 692, 19-28.
http://dx.doi.org/10.1016/j.ejphar.2012.06.045
[9] Li, M., Yu, X., Guo, H., Sun, L., Wang, A., Liu, Q., Wang, X. and Li, J. (2014) Bufalin Exerts Antitumor Effects by Inducing Cell Cycle Arrest and Triggering Apoptosis in Pancreatic Cancer Cells. Tumour Biology, 35, 2461-2471.
http://dx.doi.org/10.1007/s13277-013-1326-6
[10] Beenken, K.E., Blevins, J.S. and Smeltzer, M.S. (2003) Mutation of sarA in Staphylococcus aureus Limits Biofilm Formation. Infection and Immunity, 71, 4206-4211.
http://dx.doi.org/10.1128/IAI.71.7.4206-4211.2003           eww141222lx
标签：

评论

发表评论

此博客中的热门博文

A Comparison of Methods Used to Determine the Oleic/Linoleic Acid Ratio in Cultivated Peanut (Arachis hypogaea L.)

Cultivated peanut ( Arachis hypogaea L.) is an important oil and food crop. It is also a cheap source of protein, a good source of essential vitamins and minerals, and a component of many food products. The fatty acid composition of peanuts has become increasingly important with the realization that oleic acid content significantly affects the development of rancidity. And oil content of peanuts significantly affects flavor and shelf-life. Early generation screening of breeding lines for high oleic acid content greatly increases the efficiency of developing new peanut varieties. The objective of this study was to compare the accuracy of methods used to classify individual peanut seed as high oleic or not high oleic. Three hundred and seventy-four (374) seeds, spanning twenty-three (23) genotypes varying in oil composition (i.e. high oleic (H) or normal/not high oleic (NH) inclusive of all four peanut market-types (runner, Spanish, Valencia and Virginia), were individually tested ...
发表评论
阅读全文

Location Optimization of a Coal Power Plant to Balance Costs against Plant’s Emission Exposure

Fuel and its delivery cost comprise the biggest expense in coal power plant operations. Delivery of electricity from generation to consumers requires investment in power lines and transmission grids. Placing a coal power plant or multiple power plants near dense population centers can lower transmission costs. If a coalmine is nearby, transportation costs can also be reduced. However, emissions from coal plants play a key role in worsening health crises in many countries. And coal upon combustion produces CO 2 , SO 2 , NO x , CO, Metallic and Particle Matter (PM10 & PM2.5). The presence of these chemical compounds in the atmosphere in close vicinity to humans, livestock, and agriculture carries detrimental health consequences. The goal of the research was to develop a methodology to minimize the public’s exposure to harmful emissions from coal power plants while maintaining minimal operational costs related to electric distribution losses and coal logistics. The objective was...
发表评论
阅读全文

Evaluation of the Safety and Efficacy of Continuous Use of a Home-Use High-Frequency Facial Treatment Appliance

At present, many home-use beauty devices are available in the market. In particular, many products developed for facial treatment use light, e.g., a flash lamp or a light-emitting diode (LED). In this study, the safety of 4 weeks’ continuous use of NEWA TM , a high-frequency facial treatment appliance, every alternate day at home was verified, and its efficacy was evaluated in Japanese individuals with healthy skin aged 30 years or older who complained of sagging of the facial skin.  Transepidermal water loss (TEWL), melanin levels, erythema levels, sebum secretion levels, skin color changes and wrinkle improvement in the facial skin were measured before the appliance began to be used (study baseline), at 2 and 4 weeks after it had begun to be used, and at 2 weeks after completion of the 4-week treatment period (6 weeks from the study baseline). In addition, data obtained by subjective evaluation by the subjects themselves on a visual analog scale (VAS) were also analyzed. Fur...
发表评论
阅读全文