跳至主要内容

Probing the Cell Cycle with Flow Cytometry

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47939#.VFbv72fHRK0

Author(s)  
Flow cytometry is a versatile technique to study different aspects of the cell cycle from subpopulations of cells to detailed cell kinetic information. In this paper a basic review of cell kinetic parameters is presented followed by detailed descriptions of the different flow cytometric methodologies that can be used to extract pertinent information for a particular study. The methodologies range from simple DNA profile analysis, the use of bromodeoxyuridine to cell cycle-associated proteins such as the cyclins.
Cite this paper
Wilson, G. (2014) Probing the Cell Cycle with Flow Cytometry. Journal of Biomedical Science and Engineering, 7, 698-711. doi: 10.4236/jbise.2014.79069. 
 

[1] Howard, A. and Pelc, S.R. (1951) Nuclear Incorporation of 32P as Demonstrated by Autoradiographs. Experimental Cell Research, 2, 178-187.
http://dx.doi.org/10.1016/0014-4827(51)90083-3
[2] Howard, A. and Pelc, S.R. (1953) Synthesis of Deoxyribonucleic Acid in Normal and Irradiated Cells and Its Relation to Chromosome Breakage. Heredity, 6, 261-273.
[3] Steel, G.G. (1977) Growth Kinetics of Tumours. Clarendon Press, Oxford.
[4] Kamentsky, L.A., Melamed, M.R. and Derman, H. (1965) Spectrophotometer: New Instrument for Ultrarapid Cell Analysis. Science, 150, 630-631.
http://dx.doi.org/10.1126/science.150.3696.630
[5] Kamentsky, L.A. and Melamed, M.R. (1967) Spectrophotometric Cell Sorter. Science, 156, 1364-1365.
http://dx.doi.org/10.1126/science.156.3780.1364
[6] Fulwyler, M.J. (1965) Electronic Separation of Biological Cells by Volume. Science, 150, 910-911.
http://dx.doi.org/10.1126/science.150.3698.910
[7] Van Dilla, M.A., Trujillo, T.T., Mullaney, P.F. and Coulter, J.R. (1969) Cell Microfluorometry: A Method for Rapid Fluorescence Measurement. Science, 163, 1213-1214.
http://dx.doi.org/10.1126/science.163.3872.1213
[8] Dittrich, W. and Gohde, W. (1969) Impulse Fluorometry of Single Cells in Suspension. Zeitschrift für Naturforschung B, 24, 360-361.
[9] Crissman, H.A. and Tobey, R.A. (1974) Cell-Cycle Analysis in 20 Minutes. Science, 184, 1297-1298.
http://dx.doi.org/10.1126/science.184.4143.1297
[10] Dean, P.N. and Jett, J.H. (1974) Mathematical Analysis of DNA Distributions Derived from Flow Microfluorometry. The Journal of Cell Biology, 60, 523-527.
[11] Gray, J.W., Bogart, E., Gavel, D.T., George, Y.S. and Moore 2nd, D.H. (1983) Rapid Cell Cycle Analysis. II. Phase Durations and Dispersions from Computer Analysis of RC Curves. Cell and Tissue Kinetics, 16, 457-471.
[12] Gratzner, H.G. (1982) Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA Replication. Science, 218, 474-475.
http://dx.doi.org/10.1126/science.7123245
[13] Dolbeare, F., Gratzner, H., Pallavicini, M.G. and Gray, J.W. (1983) Flow Cytometric Measurement of Total DNA Content and Incorporated Bromodeoxyuridine. Proceedings of the National Academy of Sciences, 80, 5573-5577.
http://dx.doi.org/10.1073/pnas.80.18.5573
[14] Darzynkiewicz, Z., Crissman, H. and Jacobberger, J.W. (2004) Cytometry of the Cell Cycle: Cycling through History. Cytometry A, 58, 21-32.
http://dx.doi.org/10.1002/cyto.a.20003
[15] Steel, G.G. and Lamerton, L.F. (1969) Cell Population Kinetics and Chemotherapy. I. The Kinetics of Tumor Cell Populations. National Cancer Institute Monograph, 30, 29-42.
[16] Baserga, R. (1969) Biochemical Events in the Cell Cycle. National Cancer Institute Monograph, 30, 1-14.
[17] Sherr, C.J. (2000) Cell Cycle Control and Cancer. Harvey Lectures, 96, 73-92.
[18] Wilson, G.D. (2004) Radiation and the Cell Cycle, Revisited. Cancer Metastasis Reviews, 23, 209-225.
http://dx.doi.org/10.1023/B:CANC.0000031762.91306.b4
[19] Rew, D.A. and Wilson, G.D. (2000) Cell Production Rates in Human Tissues and Tumours and Their Significance. Part 1: An Introduction to the Techniques of Measurement and Their Limitations. European Journal of Surgical Oncology: The Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 26, 227-238.
[20] Gray, J.W., Dolbeare, F., Pallavicini, M.G., Beisker, W. and Waldman, F. (1986) Cell Cycle Analysis Using Flow Cytometry. International Journal of Radiation Biology. Related Studies in Physics, Chemistry, 49, 237-255.
http://dx.doi.org/10.1080/09553008514552531
[21] Vindelov, L. and Christensen, I.J. (1990) An Integrated Set of Methods for Routine Flow Cytometric DNA Analysis. Methods in Cell Biology, 33, 127-137.
http://dx.doi.org/10.1016/S0091-679X(08)60519-1
[22] Wilson, G.D. (2000) Analysis of DNA-Measurement of Cell Kinetics by the Bromodeoxyuridine/Anti-Bromodeoxyu- ridine Method. In: Ormerod, M.G., Ed., Flow Cytometry: A Practical Approach, IRL Press, Oxford, 159-177.
[23] Rew, D.A. and Wilson, G.D. (2000) Cell Production Rates in Human Tissues and Tumours and Their Significance. Part II: Clinical Data. European Journal of Surgical Oncology: The Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 26, 405-417.
[24] Begg, A.C., McNally, N.J., Shrieve, D.C. and Karcher, H. (1985) A Method to Measure the Duration of DNA Synthesis and the Potential Doubling Time from a Single Sample. Cytometry, 6, 620-626.
http://dx.doi.org/10.1002/cyto.990060618
[25] Wilson, G.D. (1998) Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67. Methods in Molecular Biology, 80, 355-363.
http://dx.doi.org/10.1007/978-1-59259-257-9_36
[26] Gerdes, J., Lemke, H., Baisch, H., Wacker, H.H., Schwab, U. and Stein, H. (1984) Cell Cycle Analysis of a Cell Proliferation-Associated Human Nuclear Antigen Defined by the Monoclonal Antibody Ki-67. Journal of Immunology, 133, 1710-1715.
[27] Darzynkiewicz, Z., Gong, J., Juan, G., Ardelt, B. and Traganos, F. (1996) Cytometry of Cyclin Proteins. Cytometry, 25, 1-13.
http://dx.doi.org/10.1002/(SICI)1097-0320(19960901)25:1<1::AID-CYTO1>3.0.CO;2-N
[28] Darzynkiewicz, Z., Gong, J. and Traganos, F. (1994) Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell Cycle. Methods in Cell Biology, 41, 421-435.
http://dx.doi.org/10.1016/S0091-679X(08)61732-X
[29] Gong, J., Ardelt, B., Traganos, F. and Darzynkiewicz, Z. (1994) Unscheduled Expression of Cyclin B1 and Cyclin E in Several Leukemic and Solid Tumor Cell Lines. Cancer Research, 54, 4285-4288.
[30] Gong, J., Traganos, F. and Darzynkiewicz, Z. (1993) Simultaneous Analysis of Cell Cycle Kinetics at Two Different DNA Ploidy Levels Based on DNA Content and Cyclin B Measurements. Cancer Research, 53, 5096-5099.
[31] Gong, J., Traganos, F. and Darzynkiewicz, Z. (1994) Staurosporine Blocks Cell Progression through G1 between the Cyclin D and Cyclin E Restriction Points. Cancer Research, 54, 3136-3139.
[32] Gong, J., Traganos, F. and Darzynkiewicz, Z. (1995) Threshold Expression of Cyclin E But Not D Type Cyclins Characterizes Normal and Tumour Cells Entering S Phase. Cell Proliferation, 28, 337-346.
http://dx.doi.org/10.1111/j.1365-2184.1995.tb00075.x
[33] Juan, G. and Darzynkiewicz, Z. (2001) Bivariate Analysis of DNA Content and Expression of Cyclin Proteins. Current Protocols in Cytometry, Chapter 7, Unit 7.9.
[34] Crosio, C., Fimia, G.M., Loury, R., Kimura, M., Okano, Y., Zhou, H., et al. (2002) Mitotic Phosphorylation of Histone H3: Spatio-Temporal Regulation by Mammalian Aurora Kinases. Molecular and Cellular Biology, 22, 874-885.
http://dx.doi.org/10.1128/MCB.22.3.874-885.2002
[35] Juan, G., Traganos, F., James, W.M., Ray, J.M., Roberge, M., Sauve, D.M., et al. (1998) Histone H3 Phosphorylation and Expression of Cyclins A and B1 Measured in Individual Cells during Their Progression through G2 and Mitosis. Cytometry, 32, 71-77.
http://dx.doi.org/10.1002/(SICI)1097-0320(19980601)32:2<71::AID-CYTO1>3.0.CO;2-H
[36] Friedrich, T.D., Okubo, E., Laffin, J. and Lehman, J.M. (1998) Okadaic Acid Induces Appearance of the Mitotic Epitope MPM-2 in SV40-Infected CV-1 Cells with a >G2-Phase DNA Content. Cytometry, 31, 260-264.
http://dx.doi.org/10.1002/(SICI)1097-0320(19980401)31:4<260::AID-CYTO5>3.0.CO;2-N
[37] Jacobberger, J.W., Frisa, P.S., Sramkoski, R.M., Stefan, T., Shults, K.E. and Soni, D.V. (2008) A New Biomarker for Mitotic Cells. Cytometry A, 73, 5-15.
http://dx.doi.org/10.1002/cyto.a.20501
[38] den Elzen, N. and Pines, J. (2001) Cyclin A Is Destroyed in Prometaphase and Can Delay Chromosome Alignment and Anaphase. The Journal of Cell Biology, 153, 121-136.
http://dx.doi.org/10.1083/jcb.153.1.121
[39] Parry, D.H. and O’Farrell, P.H. (2001) The Schedule of Destruction of Three Mitotic Cyclins Can Dictate the Timing of Events during Exit from Mitosis. Current Biology: CB, 11, 671-683.
http://dx.doi.org/10.1016/S0960-9822(01)00204-4             eww141103lx
标签：

评论

发表评论

此博客中的热门博文

A Comparison of Methods Used to Determine the Oleic/Linoleic Acid Ratio in Cultivated Peanut (Arachis hypogaea L.)

Cultivated peanut ( Arachis hypogaea L.) is an important oil and food crop. It is also a cheap source of protein, a good source of essential vitamins and minerals, and a component of many food products. The fatty acid composition of peanuts has become increasingly important with the realization that oleic acid content significantly affects the development of rancidity. And oil content of peanuts significantly affects flavor and shelf-life. Early generation screening of breeding lines for high oleic acid content greatly increases the efficiency of developing new peanut varieties. The objective of this study was to compare the accuracy of methods used to classify individual peanut seed as high oleic or not high oleic. Three hundred and seventy-four (374) seeds, spanning twenty-three (23) genotypes varying in oil composition (i.e. high oleic (H) or normal/not high oleic (NH) inclusive of all four peanut market-types (runner, Spanish, Valencia and Virginia), were individually tested ...
发表评论
阅读全文

Location Optimization of a Coal Power Plant to Balance Costs against Plant’s Emission Exposure

Fuel and its delivery cost comprise the biggest expense in coal power plant operations. Delivery of electricity from generation to consumers requires investment in power lines and transmission grids. Placing a coal power plant or multiple power plants near dense population centers can lower transmission costs. If a coalmine is nearby, transportation costs can also be reduced. However, emissions from coal plants play a key role in worsening health crises in many countries. And coal upon combustion produces CO 2 , SO 2 , NO x , CO, Metallic and Particle Matter (PM10 & PM2.5). The presence of these chemical compounds in the atmosphere in close vicinity to humans, livestock, and agriculture carries detrimental health consequences. The goal of the research was to develop a methodology to minimize the public’s exposure to harmful emissions from coal power plants while maintaining minimal operational costs related to electric distribution losses and coal logistics. The objective was...
发表评论
阅读全文

Evaluation of the Safety and Efficacy of Continuous Use of a Home-Use High-Frequency Facial Treatment Appliance

At present, many home-use beauty devices are available in the market. In particular, many products developed for facial treatment use light, e.g., a flash lamp or a light-emitting diode (LED). In this study, the safety of 4 weeks’ continuous use of NEWA TM , a high-frequency facial treatment appliance, every alternate day at home was verified, and its efficacy was evaluated in Japanese individuals with healthy skin aged 30 years or older who complained of sagging of the facial skin.  Transepidermal water loss (TEWL), melanin levels, erythema levels, sebum secretion levels, skin color changes and wrinkle improvement in the facial skin were measured before the appliance began to be used (study baseline), at 2 and 4 weeks after it had begun to be used, and at 2 weeks after completion of the 4-week treatment period (6 weeks from the study baseline). In addition, data obtained by subjective evaluation by the subjects themselves on a visual analog scale (VAS) were also analyzed. Fur...
发表评论
阅读全文