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Optimization of Solid Phase Microextraction Procedure Followed by Gas Chromatography with Electron Capture Detector for Pesticides Butachlor and Chlorpyrifos

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In this study, headspace solid phase microextraction (HS-SPME) followed by gas chromatography using electron capture detection system (GC-ECD) were developed for the determination of chloraacetanilide (butachlor) and chlorpyrifos presented in biological samples. Different parameters affecting the extraction procedure were optimized including extraction time (30 minutes), extraction temperature (80°C), sample volume (3 mL), sample pH (2), added NaCl (0.3 gram) and sample stirring rate (400 rpm). Different concentrations of 1 - 100 ng/ml were applied for butachlor and a linear calibration curve was obtained. Furthermore, a similar linearity was obtained for chlorpyrifos, using a concentration range of 1 - 250 ng/ml. The limit of detection (LOD) obtained for butachlor and chlorpyrifos were 0.088 and 0.53 ng/ml respectively. The optimized methods for both compounds were validated using two concentrations of 25 and 50 ng/ml in spiked urine samples. Obtained recoveries of spiked urine samples were 83.06% - 99.8% with RSD of lower than 11%. Optimized technique was simple, inexpensive, solvent free and fast in comparison with other conventional methods and had compatibility with the chromatographic analytical system. This method offers low detection limits to analyze pesticides in urine samples that are very important in the exposure monitoring in occupational health.
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Ghavidel, F. , Shahtaheri, S. , Jazani, R. , Torabbeigi, M. , Froushani, A. and Khadem, M. (2014) Optimization of Solid Phase Microextraction Procedure Followed by Gas Chromatography with Electron Capture Detector for Pesticides Butachlor and Chlorpyrifos. American Journal of Analytical Chemistry, 5, 535-546. doi: 10.4236/ajac.2014.59061. 
 

[1] Hsu, J.P., Herbert, G., Wheeler, D.E., Camann, H.J., et al. (1988) Analytical Methods for Detection of Nonoccupational Exposure to Pesticides. Journal of Chromatographic Science, 26, 181-189.
http://dx.doi.org/10.1093/chromsci/26.4.181
[2] Matsumura, F. (1985) Toxicology of Insecticides. 2nd Edition, Plenum, New York.
http://dx.doi.org/10.1007/978-1-4613-2491-1
[3] Ritter, L., Goushleff, N.C.I., Arbuckle, T., Cole, D., Donald, M. and Raizenne, M. (2006) Addressing the Linkage between Exposure to Pesticides and Human Health Effects—Research Trends and Priorities for Research. Journal of Toxicology and Environmental Health, 9, 441-456.
http://dx.doi.org/10.1080/10937400600755895
[4] Farombi, O.E., Ajimoko, Y.R. and Adelowo, O.A. (2008) Effect of Butachlor on Antioxidant Enzyme Status and Lipid Peroxidation in Fresh Water African Catfish, (Clarias gariepinus). International Journal of Environmental Research and Public Health, 5, 423-427.
http://dx.doi.org/10.3390/ijerph5050423
[5] Aktar, W., Sengupta, D. and Chowdhury, A. (2009) Impact of Pesticides Use in Agriculture: Their Benefits and Hazards. Interdisciplinary Toxicology, 2, 1-12.
http://dx.doi.org/10.2478/v10102-009-0001-7
[6] Costa, L.G. (2006) Current Issues in Organophosphate Toxicology. Clinica Chimica Acta, 366, 1-13.
http://dx.doi.org/10.1016/j.cca.2005.10.008
[7] Otero, R., Santiago Silva, M. and Grimalt, J.O. (1997) Hexachlorocyclohexanes in Human Blood Serum. Journal of Chromatography A, 778, 87-94.
http://dx.doi.org/10.1016/S0021-9673(97)00339-7
[8] Burse, V.W., Head, S.L., Korver, M.P., McClure, P.C., Donahue, J.F. and Needham, L.L. (1990) Determination of Selected Organochlorine Pesticides and Polychlorinated Biphenyls in Human Serum. Journal of Analytical Toxicology, 14, 137-142.
http://dx.doi.org/10.1093/jat/14.3.137
[9] Pico, Y., Font, G., Molto, J.C. and Manes, J. (2000) Pesticide Residue Determination in Fruit and Vegetables by Liquid Chromatography-Mass Spectrometry. Journal of Chromatography A, 882, 153-173.
http://dx.doi.org/10.1016/S0021-9673(00)00294-6
[10] Jimenez, J.J., Bernal, J.L., del Nozal, M.J., Novo, M., Higes, M. and Llorente, J. (2000) Determination of Rotenone Residues in Raw Honey by Solid-Phase Extraction and High-Performance Liquid Chromatography. Journal of Chromatography A, 871, 67-73.
http://dx.doi.org/10.1016/S0021-9673(99)01063-8
[11] Tsydenova, O.V., Sudaryanto, A., Kajiwara, N., Kunisue, T., Batoev, V.B. and Tanabe, S. (2007) Organohalogen Compounds in Human Breast Milk from Republic of Buryatia, Russia. Environmental Pollution, 146, 225-235.
http://dx.doi.org/10.1016/j.envpol.2006.04.036
[12] Shahtaheri, S.J., Abdollahi, M., Golbabaei, F., Rahimi-Froushani, A. and Ghamari, F. (2004) Optimization of SPE for Analysis of Mandelic Acid as a Biomarker of Exposure to Ethyl Benzene. Iranian Journal of Environmental Health Science & Engineering, 1, 70-80.
[13] Shahtaheri, S.J., Khadem, M., Golbabaei, F. and Rahimi-Froushan, A. (2006) Pre-Concentration of Cadmium Using Amberlite XAD-4 Prior to Atomic Absorption Spectroscopy. Iranian Journal of Environmental Health Science & Engineering, 3, 45-52.
[14] Shahtaheri, S.J., Khadem, M., Golbabaei, F. and Rahimi-Froushani, A. (2007) Optimization of Sample Preparation Procedure for Evaluation of Occupational and Environmental Exposure to Nickel. Iranian Journal of Public Health, 36, 73-81.
[15] Shahtaheri, S.J., Ghamari, F., Golbabaei, F., Rahimi-Froushani, A. and Abdollahi, M. (2005) Sample Preparation Followed by High Performance Liquid Chromatographic (HPLC) Analysis for Monitoring of Muconic Acid as a Biomarker of Occupational Exposure to Benzene. International Journal of Occupational Safety and Ergonomics, 11, 337-388.
[16] Shahtaheri, S.J., Ibrahimi, L., Golbabaei, F. and Hosseini, M. (2007) Solid Phase Extraction for 1-Hydroxypyrene as a Biomarker of Exposure to PAHs Prior to High Performance Liquid Chromatography. Iranian Journal of Chemistry and Chemical Engineering, 26, 75-81.
[17] Shahtaheri, S.J., Abdollahi, M., Golbabaei, F. and Rahimi-Froushani, A. (2008) Monitoring of Mandelic Acid as a Biomarker of Environmental and Occupational Exposures to Styrene. International Journal of Environmental Research, 2, 169-176.
[18] Barnabas, I.J. and Dean, J.R. (1994) Owen Supercritical Fluid Extraction of Analytes from Environmental Samples, A Review. Analyst, 119, 2381-2394.
http://dx.doi.org/10.1039/an9941902381
[19] Viana, E., Molto, J.C. and Font, G. (1996) Optimization of a Matrix Solid-Phase Dispersion Method for the Analysis of Pesticide Residues in Vegetables. Journal of Chromatography A, 754, 437-444.
http://dx.doi.org/10.1016/S0021-9673(96)00538-9
[20] Barrionuevo, W.R. and Lancas, F.M. (2002) Comparison of Liquid-Liquid Extraction (LLE), Solid-Phase Extraction (SPE), and Solid-Phase Microextraction (SPME) for Pyrethroid Pesticides Analysis from Enriched River Water. Bulletin of Environmental Contamination and Toxicology, 69, 123-128.
http://dx.doi.org/10.1007/s00128-002-0018-5
[21] Zhang, Z. and Pawliszyn, J. (1993) Headspace Solid Phase Microextraction. Analytical Chemistry, 65, 1843-1852.
http://dx.doi.org/10.1021/ac00062a008
[22] Pan, L. and Pawliszyn, J. (1997) Derivatization/Solid-Phase Microextraction: New Approach to Polar Analytes. Analytical Chemistry, 69, 196-205.
http://dx.doi.org/10.1021/ac9606362
[23] Tsoukali, H., Raikos, N., Theodoridis, G. and Psaroulis, D. (2004) Headspace Solid Phase Microextraction for the Gas Chromatographic Analysis of Methyl-Parathion in Post-Mortem Human Samples. Forensic Science International, 143, 127-132.
http://dx.doi.org/10.1016/j.forsciint.2004.02.032
[24] Heidari, H.R., Shahtaheri, S.J., Golbabaei, F., Alimohamadi, M. and Rahimi-Froushani, A. (2008) Optimization of Headspace Solid Phase Microextraction Procedure for Trace Analysis of Toluene. International Journal of Occupational Safety and Ergonomics (JOSE), 14, 395-405.
[25] Heidari, H.R., Shahtaheri, S.J., Golbabaei, F., Alimohamadi, M. and Rahimi-Froushani, A. (2009) Trace Analysis of Xylene in Occupational Exposures Monitoring. Iranian Journal of Public Health, 38, 89-99.
[26] Hernandez, F., Pitarch, E., Beltran, J. and Lopez, F.J. (2002) Headspace Solid-Phase Microextraction in Combination with Gas Chromatography and Tandem Mass Spectrometry for the Determination of Organochlorine and Organophosphorus Pesticides in Whole Human Blood. Journal of Chromatography B, 769, 65-77.
http://dx.doi.org/10.1016/S1570-0232(01)00627-4
[27] Kin, C.M. and Huat, T. (2010) Headspace Solid-Phase Microextraction for the Evaluation of Pesticide Residue Contents in Cucumber and Strawberry after Washing Treatment. Food Chemistry, 123, 760-764.
http://dx.doi.org/10.1016/j.foodchem.2010.05.038
[28] Alvarez, M.F., Llompart, M., Lamasa, J.P., Lores, M., Garcia-Jares, C., Cela, R. and Dagnac, T. (2008) Development of a Solid-Phase Microextraction Gas Chromatography with Microelectron-Capture Detection Method for a Multiresidue Analysis of Pesticides in Bovine Milk. Analytica Chimica Acta, 617, 37-50.
http://dx.doi.org/10.1016/j.aca.2008.01.021
[29] Beceiro-Gonzalez, E., Concha-Grana, E., Guimaraes, A., Goncalves, C., Muniategui-Lorenzo, S. and Alpendurada, M.F. (2007) Optimisation and Validation of a Solid-Phase Microextraction Method for Simultaneous Determination of Different Types of Pesticides in Water by Gas Chromatography-Mass Spectrometry. Journal of Chromatography A, 1141, 165-173.
http://dx.doi.org/10.1016/j.chroma.2006.12.042
[30] Namera, A., Watanabe, T., Yashiki, M., Iwasaki, Y. and Kojima, T. (1999) Simple Analysis of Arylamide Herbicides in Serum Using Headspace-Solid Phase Microextraction and GC/MS. Forensic Science International, 103, 217-226.
http://dx.doi.org/10.1016/S0379-0738(99)00088-2
[31] Tsoukali, H., Theodoridis, G., Raikos, N. and Grigoratou, I. (2005) Solid Phase Microextraction Gas Chromatographic Analysis of Organophosphorus Pesticides in Biological Samples. Journal of Chromatography B, 822, 194-200.
http://dx.doi.org/10.1016/j.jchromb.2005.05.035
[32] Yao, Z.W., Jiang, G.B., Liu, J.M. and Cheng, W. (2001) Application of Solid-Phase Microextraction for the Determination of Organophosphorous Pesticides in Aqueous Samples by Gas Chromatography with Flame Photometric Detector. Talanta, 55, 807-814.
http://dx.doi.org/10.1016/S0039-9140(01)00504-5
[33] Bicchi, C., Cordero, C., Liberto, E., Sgorbini, B. and Rubiolo, P. (2007) Reliability of Fibres in Solid Phase Microextraction for Routine Analysis of the Headspace of Aromatic and Medicinal Plants. Journal of Chromatography A, 1152, 138-149.
http://dx.doi.org/10.1016/j.chroma.2007.02.011
[34] Sousa, E.T., Rodrigues, F.M., Martins, C.C., de Oliveira, F.S., Pereira, P.A.P. and de Andrade, J.B. (2006) Multivariate Optimization and HS-SPME/GC-MS Analysis of VOCs in Red, Yellow and Purple Varieties of Capsicum chinese sp. Peppers. Microchemical Journal, 82, 142-149.
http://dx.doi.org/10.1016/j.microc.2006.01.017
[35] Sakamoto, M. and Tsutsumi, T. (2004) Applicability of Headspace Solid-Phase Microextraction to the Determination of Multi-Class Pesticides in Waters. Journal of Chromatography A, 1028, 63-74.
http://dx.doi.org/10.1016/j.chroma.2003.11.066
[36] Doong, R.A., Chang, S.M. and Sun, Y.C. (2000) Solid-Phase Microextraction for Determining the Distribution of Sixteen US Environmental Protection Agency Polycyclic Aromatic Hydrocarbons in Water Samples. Journal of Chromatography A, 879, 177-188.
http://dx.doi.org/10.1016/S0021-9673(00)00347-2
[37] Fernandez Alvarez, M., Liompart, M., Pablo Lamas, J., Lores, M., Garcia-Jares, C., Cela, R. and Dagnac, T. (2008) Development of a Solid Phase Microextraction Gas Chromatography with Microelectron-Capture Detection Method for a Multiresidue Analysis of Pesticides in Bovine Milk. Analytica Chimica Acta, 617, 37-50.
http://dx.doi.org/10.1016/j.aca.2008.01.021
[38] Kataoka, H., Lord, H.L. and Pawliszyn, J. (2000) Applications of Solid Phase Microextraction in Food Analysis. Journal of Chromatography A, 880, 35-62.
http://dx.doi.org/10.1016/S0021-9673(00)00309-5
[39] Gorecki, T. and Pawliszyn, J. (1997) Effect of Sample Volume on Quantitative Analysis by Solid-Phase Microextraction. Analyst, 122, 1079-1086.
http://dx.doi.org/10.1039/a701303e
[40] Xua, X.Q., Yang, H.H., Wang, L., Han, B., Wang, X.R. and Lee, F. (2007) Analysis of Chloroacetanilide Herbicides in Water Samples by Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry. Analytica Chimica Acta, 591, 87-96.
http://dx.doi.org/10.1016/j.aca.2007.03.044                eww141112lx
 
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