On the Question of Defining the Association Constants by the Method of Fluorescence Quenching

Read full paper at:
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=51704#.VHVl12fHRK0

Author(s)

Nikolay L. Lavrik, Nikolay M. Bazhin

Affiliation(s)

Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russian Federation.

ABSTRACT

A study is made on the previously ignored problem of the dependence of a static fluorescence quenching Stern-Volmer constant K_sv on the initial concentration of [F]₀ fluorophore F. This correlation is shown to exist. It is concluded that the Stern-Volmer quenching constant may be used as association constant K only with .

KEYWORDS

Fluorescence Quenching, Association Constants, Stern-Volmer Constant

Cite this paper

Lavrik, N. and Bazhin, N. (2014) On the Question of Defining the Association Constants by the Method of Fluorescence Quenching. American Journal of Analytical Chemistry, 5, 1065-1068. doi: 10.4236/ajac.2014.516113.

 

References

[1]	De Weert, M. and Stella, L. (2011) Fluorescence Quenching and Ligand Binding: A Critical Discussion of a Popular Methodology. Journal of Molecular Structure, 998, 144-150. http://dx.doi.org/10.1016/j.molstruc.2011.05.023
[2]	Lakovicz, J. (2010) Principles of Fluorescence Spectroscopy. 3rd Edition, Springer, Berlin.                                       eww141126lx