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Genetic Diversity and Population Structure of North China Mountain Walnut Revealed by ISSR

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http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50855#.VE3aWlfHRK0

North China Mountain Walnut (NCMW) is one of the ancestors of extant cultivated species, and a valuable gene resource for resistance breeding of walnut in China. Inter-Simple Sequence Repeat (ISSR) primers were designed to evaluate the level and pattern of genetic diversity in eight populations of NCMW. Nine ISSR primers yielded 91 amplification products with different sizes, of which 84 (92.31%) were polymorphic. A high species-level genetic diversity was detected with Nei’s (H = 0.2592) and Shannon’s diversity (I = 0.4003). In contrast, the population-level genetic diversity was relatively lower (PPB = 43.27%, H = 0.1347, I = 0.1862). Coefficient of populations differentiation (GST) was 0.5066, indicating that inter-population and intra-population variation contributed 50.66% and 49.34% respectively to the total genetic variability. This relative level of variation was further supported by AMOVA analysis. Limited gene flow (Nm = 0.5133.), habitat fragmentation and geographical isolation might be responsible for the population structure of NCMW. UPGMA cluster analysis classified the eight populations into three groups which showed no significant relationship between the genetic similarity coefficient and geographic origin but showed remarkable association with morpho-physiological characters, particularly nut traits. The results of the study provide species-level and population-level genetic profiles for further exploitation and conservation of genetic diversity of NCMW.
Cite this paper
Ji, A. , Wang, Y. , Wu, G. , Wu, W. , Yang, H. and Wang, Q. (2014) Genetic Diversity and Population Structure of North China Mountain Walnut Revealed by ISSR. American Journal of Plant Sciences, 5, 3194-3202. doi: 10.4236/ajps.2014.521335. 
 

[1] Wu, G.L., Liu, Q.L., Zheng, X.B., Song, Y.Q., Jian, Z.H. and Peng, G.B. (2009) Advances in Research on the Worldwide Walnut Germplasmm. Journal of Fruit Science, 26, 539-545. (In Chinese)
[2] Ebrahimi, A., Fatahi, R. and Zamani, Z. (2011) Analysis of Genetic Diversity among Some Persian walnut Genotypes (Juglans regia L.) Using Morphological Traits and SSRs Markers. Scientia Horticulturae, 130, 146-151.
http://dx.doi.org/10.1016/j.scienta.2011.06.028
[3] Xi, S.K. (1987) Gene Resources of Juglans and Genetic Improvement of Juglans regia in China. Scientia Silvae Sinicae, 23, 342-349. (In Chinese)
[4] Fjellstrom, R.G., Parfitt, D.E. and McGranahan, G.H. (1994) Genetic Relationship and Characterization of Persian walnut (Juglans regia L.) Cultivars Using Restriction Fragment Length Polymorphisms (RFLPs). Journal of the American Society for Horticultural Science, 119, 833-839.
[5] Fjellstrom, R.G. and Parfitt, D.E. (1994) Walnut (Juglans spp.) Genetic Diversity Determined by Restriction Fragment Length Polymorphisms. Genome, 37, 690-700.
http://dx.doi.org/10.1139/g94-097
[6] Nicese, F.P., Hormaza, J.I. and McGranahan, G.H. (1998) Molecular Characterization and Genetic Relatedness among Walnut (Juglans regia L.) Genotypes Based on ISSR Markers. Euphytica, 101, 199-206.
http://dx.doi.org/10.1023/A:1018390120142
[7] Wu, Y.M., Pei, D., Xi, S.K. and Li, J.R. (2000) A Study on the Genetic Relationship among Species in Juglans L. Using RAPD Markers. Acta Horticulturae Sinica, 27, 17-22. (In Chinese)
[8] Wu, Y.M., Liu, Y., Dong, F.X. and Xi, S.K. (2000) Study on Different Ecological Types of Chinese Walnut (J. regia) Using RAPD Markers. Journal of Beijing Forestry Universitty, 22, 23-27. (In Chinese)
[9] Nazeer, A., Mir, J.I., Reyazul, R.Mir., Nazir, A.R., Rizwan, R., Shabir, H.W., Wajida, S., Hidayatullah, M. and Sheikh, M.A. (2012) SSR and RAPD Analysis of Genetic Diversity in Walnut (Juglans regia L.) Genotypes from Jammu and Kashmir, India. Physiology and Molecular Biology of Plants, 18, 149-160.
http://dx.doi.org/10.1007/s12298-012-0104-z
[10] Bayazit, S., Kazan, K., Gulbitti, V., Evik, C., Ayanoglu, H. and Ergul, A. (2007) AFLP Analysis of Genetic Diversity in Low Chilling Requiring Walnut (Juglans regia L.) Genotypes from Hatay, Turkey. Scientia Horticulturae, 111, 394-398.
http://dx.doi.org/10.1016/j.scienta.2006.11.006
[11] Wang, H.X., Zhao, S.G., Gao, Y., Zhang, Z.H. and Xuan, L.C. (2011) Genetic Diversity of Juglans regia L. Cultivars Revealed by AFLP Analysis. Scientia Agricultura Sinica, 44, 1434-1442. (In Chinese)
[12] Dangl, G.S., Woeste, K., Aradhya, M.K., Koehmstedt, A., Simon, C., Potter, D., Leslie, C.A. and McGranahan, G. (2005) Characterization of 14 Microsatellite Markers for Genetic Analysis and Cultivar Identification of Walnut. Journal of the American Society for Horticultural Science, 130, 348-354.
[13] Foroni, I., Rao, R., Woeste, K. and Gallitelli, M. (2005) Characterization of (Juglans regia L.) with SSR Markers and Evaluation of Genetic Relationships among Cultivars and the “Sorrento” Landrace. The Journal of Horticultural Science and Biotechnology, 80, 49-53.
[14] Victory, E.R., Jeffrey, C., Glaubitz, O.E., Rhodes, J.R. and Woeste, K.E. (2006) Genetic Homogeneity in Juglans nigra (Juglandaceae) at Nuclear Microsatellites. American Journal of Botany, 93, 118-126.
http://dx.doi.org/10.3732/ajb.93.1.118
[15] Wang, H., Hao, J.M., Wang, B.Q. and Pei, D. (2007) SSR Analysis of Genetic Diversity of Eight Natural Walnut Populations in China. Scientia Silvae Sinicae, 43, 120-124. (In Chinese)
[16] Wang, H., Pei, D., Gu, R.S. and Wang, B.Q. (2008) Genetic Diversity and Structure of Walnut Populations in Central and Southwestern China Revealed by Microsatellite Markers. Journal of the American Society for Horticultural Science, 1332, 197-203.
[17] Wang, H. (2010) Genetic Diversity of Germplasm Resources on Walnut in Tibet Region. Thesis, Chinese Academy of Forestry Sciences, Peking, 77-78. (In Chinese)
[18] Potter, D., Gao, F., Aiello, G., Leslie, C. and McGranahan, G. (2002) Intersimple Sequence Repeat Markers for Fingerprinting and Determining Genetic Relationships of Walnut (Juglans regia) Cultivars. Journal of the American Society for Horticultural Science, 127, 75-81.
[19] Christopoulosa, M.V., Dimos, R., Eleni, T. and Penelope, J.B. (2010) Germplasm Diversity and Genetic Relationships among Walnut (Juglans regia L.) Cultivars and Greek Local Selections Revealed by Inter-Simple Sequence Repeat (ISSR) Markers. Scientia Horticulturae, 125,584-592.
http://dx.doi.org/10.1016/j.scienta.2010.05.006
[20] Li, C., Luo, S.P., Zeng, B., Li, J. and Li, G. (2011) Analysis of Genetic Diversity of Germplasm Resources of Walnut (Juglans regia L.) Revealed by ISSR in Xinjiang of China. Scientia Agricultura Sinica, 44, 1871-1879.
[21] Thimmappaiah, W.G., Santhosh, D., Shobha, G.S. and Melwyn, G.S. (2009) Assessment of Genetic Diversity in Cashew Germplasm Using RAPD and ISSR Markers. Scientia Horticulturae, 120, 411-417.
http://dx.doi.org/10.1016/j.scienta.2008.11.022
[22] Ai, C.X., Zhang, L.S., Wei, H.R., Jin, S.N., Yuan, K.J. and Liu, Q.Z. (2007) Study on the Genetic Diversity of Natural Chestnut of Shandong by ISSR. Chinese Journal of Biotechnology, 23, 628-633. (In Chinese)
http://dx.doi.org/10.1016/S1872-2075(07)60043-0
[23] Kamalesh, S.M., Tikam, S.R. and Shirish, A.R. (2011) Molecular Analyses of Genetic Variability in Soap Nut (Sapindus mukorossi Gaertn.). Industrial Crops and Products, 34, 1111-1118.
http://dx.doi.org/10.1016/j.indcrop.2011.03.029
[24] Yeh, F.C., Yang, R.C. and Boyle, T. (1999) POPGENE Version 1.32: Microsoft Window-Based Freeware for Population Genetics Analysis. University of Alberta, Edmonton.
[25] Nei, M. (1973) Analysis of Gene Diversity in Subdivided Populations. Proceedings of the National Academy of Sciences of the United States of America, 70, 3321-3323.
http://dx.doi.org/10.1073/pnas.70.12.3321
[26] Rohlf, F.J. (2000) NTSYS-Pc: Numerical Taxonomy and Multivariate Analysis System. Version 2.1., Exeter Publishing Led., New York.
[27] Nei, M. (1978) Estimation of Average Heterozygosity and Genetic Distance from a Small Number of Individuals. Genetics, 89, 583-590.
[28] Excoffier, L., Smouse, P. and Quattro, J.M. (1992) Analysis of Molecular Variance Inferred from Metric Distances among DNA Haplotypes: Application to Human Mitochondrial DNA Restriction Data. Genetics, 131, 479-491.
[29] Ge, Y.Q., Qiu, Y.X., Ding, B.Y. and Fu, C.X. (2003) An ISSR Analysis on Population Genetic Diversity of the Relict Plant Ginkgo biloba. Biodiversity Science, 11, 276-287. (In Chinese)
[30] Li, J.M. and Jin, Z.C. (2008) Genetic Structure of Endangered Emmenopterys henryi Oliv. Based on ISSR Polymorphism and Implications for Its Conservation. Genetica, 133, 227-234.
http://dx.doi.org/10.1007/s10709-007-9204-z
[31] Li, G.T., Ai, C.X., Zhang, L.S., Wei, H.R. and Liu, Q.Z. (2011) ISSR Analysis of Genetic Diversity among Seedling Walnut (Juglans spp.) Populations. Journal of Plant Genetic Resources, 12, 640-645. (In Chinese)
[32] Freville, H., Justy, F. and Olivieri, I. (2001) Comparative Allozyme and Microsatellite Population Structure in a Narrow Endemic Plant Species, Centaurea corymbosa Pourret (Asteraceae). Molecular Ecology, 10, 879-889.
http://dx.doi.org/10.1046/j.1365-294X.2001.01249.x
[33] Maguire, T.L., Peakall, R. and Saenger, P. (2002) Comparative Analysis of Genetic Diversity in the Mangrove Species Avicennia marina (Forsk.) Vierh. (Avicenniaceae) Detected by AFLPs and SSRs. Theoretical and Applied Genetics, 104, 388-398.
http://dx.doi.org/10.1007/s001220100724                   eww141027lx
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